Open Access

Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele

  • Authors:
    • Natalia S. Carvalho
    • Antonio F. Moron
    • Ramkumar Menon
    • Sergio Cavalheiro
    • Mauricio M. Barbosa
    • Herbene J. Milani
    • Marcia M. Ishigai
  • View Affiliations

  • Published online on: August 21, 2017     https://doi.org/10.3892/etm.2017.4976
  • Pages: 3732-3736
  • Copyright: © Carvalho et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

An increased understanding of the reparative process in fetal membrane following surgical techniques may be helpful to decrease the risks to mother and fetus and avoid adverse pregnancy outcomes. The present study discusses histological evaluation of the fetal membrane following open fetal surgery. Chorioamniotic membranes (n=10) were obtained following birth from pregnancies that underwent open fetal surgery for myelomeningocele. The collagen distribution was quantified using picrosirius‑polarization method comparing the suture site with non‑suture site. The differences between the collagen fiber percentages at the two sites was evaluated by the paired t‑test with P<0.05. The mean gestational age of fetal surgery was 26.09±0.3 and 33.81±0.82 weeks at birth. The picrosirius red sign was more intense at the suture site, primarily associated with collagen type 1. Collagen observed in the surgical area was significantly increased (13.22±2.84%) compared with the non‑surgical area (6.16±1.09%; P<0.0001). It was observed that the reparative activity at the suture site of the fetal membrane was characterized by a significant increase in collagen fibers. The findings suggest nascent collagen synthesis, tissue remodeling and repair of suture site, a mechanism likely to prevent the amniotic fluid leakage and maintain pregnancy following open fetal surgery.

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October-2017
Volume 14 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Carvalho NS, Moron AF, Menon R, Cavalheiro S, Barbosa MM, Milani HJ and Ishigai MM: Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele. Exp Ther Med 14: 3732-3736, 2017
APA
Carvalho, N.S., Moron, A.F., Menon, R., Cavalheiro, S., Barbosa, M.M., Milani, H.J., & Ishigai, M.M. (2017). Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele. Experimental and Therapeutic Medicine, 14, 3732-3736. https://doi.org/10.3892/etm.2017.4976
MLA
Carvalho, N. S., Moron, A. F., Menon, R., Cavalheiro, S., Barbosa, M. M., Milani, H. J., Ishigai, M. M."Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele". Experimental and Therapeutic Medicine 14.4 (2017): 3732-3736.
Chicago
Carvalho, N. S., Moron, A. F., Menon, R., Cavalheiro, S., Barbosa, M. M., Milani, H. J., Ishigai, M. M."Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele". Experimental and Therapeutic Medicine 14, no. 4 (2017): 3732-3736. https://doi.org/10.3892/etm.2017.4976