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Article

Effect of testosterone and hypoxia on the expansion of umbilical cord blood CD34+ cells in vitro

  • Authors:
    • Liping Zhou
    • Xiaowei Zhang
    • Panpan Zhou
    • Xue Li
    • Xuejing Xu
    • Qing Shi
    • Dong Li
    • Xiuli Ju
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China, Department of Pediatrics, The Sixth People's Hospital of Jinan, Jinan, Shandong 250200, P.R. China
  • Pages: 4467-4475
    |
    Published online on: August 24, 2017
       https://doi.org/10.3892/etm.2017.5026
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Abstract

Successfully expanding hematopoietic stem cells (HSCs) is advantageous for clinical HSC transplantation. The present study investigated the influence of testosterone on the proliferation, antigen phenotype and expression of hematopoiesis‑related genes in umbilical cord blood‑derived cluster of differentiation (CD)34+ cells under normoxic or hypoxia conditions. Cord blood (CB) CD34+ cells were separated using magnetic activated cell sorting. A cytokine cocktail and feeder cells were used to stimulate the expansion of CD34+ cells under normoxic (20% O2) and hypoxic (1% O2) conditions for 7 days and testosterone was added accordingly. Cells were identified using flow cytometry and reconstruction capacity was determined using a colony‑forming unit (CFU) assay. The effects of oxygen concentration and testosterone on the expression of hematopoietic‑related genes, including homeobox (HOX)A9, HOXB2, HOXB4, HOXC4 and BMI‑1, were measured using reverse transcription‑quantitative polymerase chain reaction. The results indicated that the number of CFUs and total cells in the testosterone group increased under normoxic and hypoxic conditions compared with the corresponding control groups. Furthermore, the presence of testosterone increased the number of CFU‑erythroid colonies. In liquid culture, the growth of CD34+ cells was rapid under normoxic conditions compared with under hypoxic conditions, however CD34+ cells were maintained in an undifferentiated state under hypoxic conditions. The addition of testosterone under hypoxia promoted the differentiation of CD34+ cells into CD34+CD38+CD71+ erythroid progenitor cells. Furthermore, it was determined that the expression of hematopoietic‑related genes was significantly increased (P<0.05) in the hypoxia testosterone group compared with the other groups. Therefore, the results of the current study indicate that a combination of hypoxia and testosterone may be a promising cultivation condition for HSC/hemopoietic progenitor cell expansion ex vivo.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou L, Zhang X, Zhou P, Li X, Xu X, Shi Q, Li D and Ju X: Effect of testosterone and hypoxia on the expansion of umbilical cord blood CD34+ cells in vitro. Exp Ther Med 14: 4467-4475, 2017.
APA
Zhou, L., Zhang, X., Zhou, P., Li, X., Xu, X., Shi, Q. ... Ju, X. (2017). Effect of testosterone and hypoxia on the expansion of umbilical cord blood CD34+ cells in vitro. Experimental and Therapeutic Medicine, 14, 4467-4475. https://doi.org/10.3892/etm.2017.5026
MLA
Zhou, L., Zhang, X., Zhou, P., Li, X., Xu, X., Shi, Q., Li, D., Ju, X."Effect of testosterone and hypoxia on the expansion of umbilical cord blood CD34+ cells in vitro". Experimental and Therapeutic Medicine 14.5 (2017): 4467-4475.
Chicago
Zhou, L., Zhang, X., Zhou, P., Li, X., Xu, X., Shi, Q., Li, D., Ju, X."Effect of testosterone and hypoxia on the expansion of umbilical cord blood CD34+ cells in vitro". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4467-4475. https://doi.org/10.3892/etm.2017.5026
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou L, Zhang X, Zhou P, Li X, Xu X, Shi Q, Li D and Ju X: Effect of testosterone and hypoxia on the expansion of umbilical cord blood CD34+ cells in vitro. Exp Ther Med 14: 4467-4475, 2017.
APA
Zhou, L., Zhang, X., Zhou, P., Li, X., Xu, X., Shi, Q. ... Ju, X. (2017). Effect of testosterone and hypoxia on the expansion of umbilical cord blood CD34+ cells in vitro. Experimental and Therapeutic Medicine, 14, 4467-4475. https://doi.org/10.3892/etm.2017.5026
MLA
Zhou, L., Zhang, X., Zhou, P., Li, X., Xu, X., Shi, Q., Li, D., Ju, X."Effect of testosterone and hypoxia on the expansion of umbilical cord blood CD34+ cells in vitro". Experimental and Therapeutic Medicine 14.5 (2017): 4467-4475.
Chicago
Zhou, L., Zhang, X., Zhou, P., Li, X., Xu, X., Shi, Q., Li, D., Ju, X."Effect of testosterone and hypoxia on the expansion of umbilical cord blood CD34+ cells in vitro". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4467-4475. https://doi.org/10.3892/etm.2017.5026
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