Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
November-2017 Volume 14 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2017 Volume 14 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Influence of reactive oxygen species on secretory component in the intestinal epithelium during hyperoxia

  • Authors:
    • Min Zhao
    • Shimiao Tang
    • Junchi Xin
    • Dongyan Liu
  • View Affiliations / Copyright

    Affiliations: Medical Research Center, Shengjing Hospital, China Medical University, Shenyang, Liaoning 110000, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4033-4040
    |
    Published online on: August 24, 2017
       https://doi.org/10.3892/etm.2017.5027
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Redox imbalance is established in various human diseases. Treatment of intestinal epithelial cells with hyperoxia for a prolonged period of time may cause serious effects on redox balance. Secretory component (SC) protein is secreted by intestinal epithelial cells, and has a vital role in mucosal immune systems and intestinal defense. The present study aimed to investigate the influence of reactive oxygen species (ROS) on intestinal epithelial cells and intestinal epithelial SC protein under hyperoxic conditions. Caco‑2 cells were treated with increasing concentrations of hydrogen peroxide (H2O2) or 85% O2 (hyperoxia) for 24 h. Flow cytometry, immunohistochemistry staining, western blot analysis and reverse transcription‑quantitative polymerase chain reaction were performed to detect the expression levels of SC protein. Significantly increased apoptosis and mortality rates were observed in hyperoxia‑ and H2O2‑treated Caco‑2 cells, as compared with the untreated control cells (P<0.05). Protein and mRNA expression levels of SC were significantly increased in hyperoxia‑ and H2O2‑treated groups, as compared with the control group (P<0.05). During hyperoxia, intestinal epithelial cells were destroyed and ROS levels increased. Therefore, the results of the present study suggested that ROS might have an important role in intestinal injury in hyperoxic environments.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Yee M, Buczynski BW and O'Reilly MA: Neonatal hyperoxia stimulates the expansion of alveolar epithelial type II cells. Am J Respir Cell Mol Biol. 50:757–766. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Steer JH, Mann TS, Lo SZ, Inglis JJ, Yap HS, Henry PJ and Joyce DA: Early induction of uncoupling protein-2 in pulmonary macrophages in hyperoxia-associated lung injury. Inhal Toxicol. 25:544–552. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Hong Y, Sun LI, Sun R, Chen H, Yu Y and Xie K: Combination therapy of molecular hydrogen and hyperoxia improves survival rate and organ damage in a zymosan-induced generalized inflammation model. Exp Ther Med. 11:2590–2596. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Chowdhury Roy S, Sengupta S, Biswas S, Sinha TK, Sen R, Basak RK, Adhikari B and Bhattacharyya A: Bacterial fucose-rich polysaccharide stabilizes MAPK-mediated Nrf2/Keap1 signaling by directly scavenging reactive oxygen species during hydrogen peroxide-induced apoptosis of human lung fibroblast cells. PLoS One. 9:e1136632014. View Article : Google Scholar : PubMed/NCBI

5 

Giorgio M, Trinei M, Migliaccio E and Pelicci PG: Hydrogen peroxide: A metabolic by-product or a common mediator of ageing signals? Nat Rev Mol Cell Biol. 8:722–728. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Carnesecchi S, Deffert C, Pagano A, Garrido-Urbani S, Métrailler-Ruchonnet I, Schäppi M, Donati Y, Matthay MA, Krause KH and Argiroffo Barazzone C: NADPH oxidase-1 plays a crucial role in hyperoxia-induced acute lung injury in mice. Am J Respir Crit Care Med. 180:972–981. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Crane DD, Bauler TJ, Wehrly TD and Bosio CM: Mitochondrial ROS potentiates indirect activation of the AIM2 inflammasome. Front Microbiol. 5:4382014. View Article : Google Scholar : PubMed/NCBI

8 

Oparka M, Walczak J, Malinska D, van Oppen LM, Szczepanowska J, Koopman WJ and Wieckowski MR: Quantifying ROS levels using CM-H2DCFDA and HyPer. Methods. 109:3–11. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Ishibashi T: Molecular hydrogen: New antioxidant and anti-inflammatory therapy for rheumatoid arthritis and related diseases. Curr Pharm Des. 19:6375–6381. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Cai X, Chen X, Wang X, Xu C, Guo Q, Zhu L, Zhu S and Xu J: Pre-protective effect of lipoic acid on injury induced by H2O2 in IPEC-J2 cells. Mol Cell Biochem. 378:73–81. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Rhee SG: Cell signaling. H2O2, a necessary evil for cell signaling. Science. 312:1882–1883. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Li Z, Choo-Wing R, Sun H, Sureshbabu A, Sakurai R, Rehan VK and Bhandari V: A potential role of the JNK pathway in hyperoxia-induced cell death, myofibroblast transdifferentiation and TGF-β1-mediated injury in the developing murine lung. BMC Cell Biol. 12:542011. View Article : Google Scholar : PubMed/NCBI

13 

Mikami Y, Iwase T, Komiyama Y, Matsumoto N, Oki H and Komiyama K: Secretory leukocyte protease inhibitor inhibits expression of polymeric immunoglobulin receptor via the NF-κB signaling pathway. Mol Immunol. 67:568–574. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Liu DY, Jiang T, Wang S and Cao X: Effect of hyperoxia on pulmonary SIgA and its components, IgA and SC. J Clin Immunol. 33:1009–1017. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Macpherson AJ, McCoy KD, Johansen FE and Brandtzaeg P: The immune geography of IgA induction and function. Mucosal Immunol. 1:11–22. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Mostov KE and Deitcher DL: Polymeric immunoglobulin receptor expressed in MDCK cells transcytoses IgA. Cell. 46:613–621. 1986. View Article : Google Scholar : PubMed/NCBI

17 

Johansen FE and Kaetzel CS: Regulation of the polymeric immunoglobulin receptor and IgA transport: New advances in environmental factors that stimulate pIgR expression and its role in mucosal immunity. Mucosal Immunol. 4:598–602. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Bruno ME, Frantz AL, Rogier EW, Johansen FE and Kaetzel CS: Regulation of the polymeric immunoglobulin receptor by the classical and alternative NF-κB pathways in intestinal epithelial cells. Mucosal Immunol. 4:468–478. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Davids BJ, Palm JE, Housley MP, Smith JR, Andersen YS, Martin MG, Hendrickson BA, Johansen FE, Svärd SG, Gillin FD and Eckmann L: Polymeric immunoglobulin receptor in intestinal immune defense against the lumen-dwelling protozoan parasite Giardia. J Immunol. 177:6281–6290. 2006. View Article : Google Scholar : PubMed/NCBI

20 

Perrier C, Sprenger N and Corthesy B: Glycans on secretory component participate in innate protection against mucosal pathogens. J Biol Chem. 281:14280–14287. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Kaetzel CS: The polymeric immunoglobulin receptor: Bridging innate and adaptive immune responses at mucosal surfaces. Immunol Rev. 206:83–99. 2005. View Article : Google Scholar : PubMed/NCBI

22 

Baylor AE, Diebel LN, Liberati DM, Dulchavsky SA, Diglio CA and Brown WJ: The effects of varying oxygen conditions and immunoglobulin A on barrier defense to bacterial invasion. Am Surg. 69:231–237. 2003.PubMed/NCBI

23 

Diebel LN, Liberati DM, Brown WJ, Dulchavsky SA, Painter TM, Diglio CA and Montgomery PC: Secretory immunoglobulin A blocks hypoxia-augmented bacterial passage across Madin-Darby canine kidney cell monolayers. J Trauma. 43:759–763. 1997. View Article : Google Scholar : PubMed/NCBI

24 

Torbati D, Tan GH, Smith S, Frazier KS, Gelvez J, Fakioglu H and Totapally BR: Multiple-organ effect of normobaric hyperoxia in neonatal rats. J Crit Care. 21:85–94. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Liu DY and Li JJ: Effect of hyperoxia on the intestinal IgA secretory component in neonatal rats and on intestinal epithelial cells in vitro. Braz J Med Biol Res. 43:1034–1041. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Corthesy B: Role of secretory immunoglobulin A and secretory component in the protection of mucosal surfaces. Future Microbiol. 5:817–829. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

28 

Liu B, Tan X, Liang J, Wu S, Liu J, Zhang Q and Zhu R: A reduction in reactive oxygen species contributes to dihydromyricetin-induced apoptosis in human hepatocellular carcinoma cells. Sci Rep. 4:70412014. View Article : Google Scholar : PubMed/NCBI

29 

Thannickal VJ and Fanburg BL: Reactive oxygen species in cell signaling. Am J Physiol Lung Cell Mol Physiol. 279:L1005–L1028. 2000.PubMed/NCBI

30 

Matsuzawa A and Ichijo H: Redox control of cell fate by MAP kinase: Physiological roles of ASK1-MAP kinase pathway in stress signaling. Biochim Biophys Acta. 1780:1325–1336. 2008. View Article : Google Scholar : PubMed/NCBI

31 

Woolley JF, Stanicka J and Cotter TG: Recent advances in reactive oxygen species measurement in biological systems. Trends Biochem Sci. 38:556–565. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Kang KA, Lee HC, Lee JJ, Hong MN, Park MJ, Lee YS, Choi HD, Kim N, Ko YG and Lee JS: Effects of combined radiofrequency radiation exposure on levels of reactive oxygen species in neuronal cells. J Radiat Res. 55:265–276. 2014. View Article : Google Scholar : PubMed/NCBI

33 

Shen HM and Liu ZG: JNK signaling pathway is a key modulator in cell death mediated by reactive oxygen and nitrogen species. Free Radical Biol Med. 40:928–939. 2006. View Article : Google Scholar

34 

Kajino-Sakamoto R, Omori E, Nighot PK, Blikslager AT, Matsumoto K and Ninomiya-Tsuji J: TGF-beta-activated kinase 1 signaling maintains intestinal integrity by preventing accumulation of reactive oxygen species in the intestinal epithelium. J Immunol. 185:4729–4737. 2010. View Article : Google Scholar : PubMed/NCBI

35 

Fujisawa T, Takeda K and Ichijo H: ASK family proteins in stress response and disease. Mol Biotechnol. 37:13–18. 2007. View Article : Google Scholar : PubMed/NCBI

36 

Xiao J, Deng J, Lv L, Kang Q, Ma P, Yan F, Song X, Gao B, Zhang Y and Xu J: Hydrogen peroxide induce human cytomegalovirus replication through the activation of p38-MAPK signaling pathway. Viruses. 7:2816–2833. 2015. View Article : Google Scholar : PubMed/NCBI

37 

Ma Z, Moruzzi N, Catrina SB, Grill V and Björklund A: Hyperoxia inhibits glucose-induced insulin secretion and mitochondrial metabolism in rat pancreatic islets. Biochem Biophys Res Commun. 443:223–228. 2014. View Article : Google Scholar : PubMed/NCBI

38 

Jin S, Ray RM and Johnson LR: TNF-alpha/cycloheximide-induced apoptosis in intestinal epithelial cells requires Rac1-regulated reactive oxygen species. Am J Physiol Gastrointest Liver Physiol. 294:G928–G937. 2008. View Article : Google Scholar : PubMed/NCBI

39 

Lee MK, Lu Y, Di LQ and Xu HQ: Protection of Tong-Sai-Mai Decoction against Apoptosis Induced by H2O2 in PC12 Cells: Mechanisms via Bcl-2-Mitochondria-ROS-INOS Pathway. Evid Based Complement Alternat Med. 2014:3714192014. View Article : Google Scholar : PubMed/NCBI

40 

Kina S, Nakasone T, Takemoto H, Matayoshi A, Makishi S, Sunagawa N, Liang F, Phonaphonh T and Sunakawa H: Regulation of chemokine production via oxidative pathway in HeLa cells. Mediators Inflamm. 2009:1837602009. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhao M, Tang S, Xin J and Liu D: Influence of reactive oxygen species on secretory component in the intestinal epithelium during hyperoxia. Exp Ther Med 14: 4033-4040, 2017.
APA
Zhao, M., Tang, S., Xin, J., & Liu, D. (2017). Influence of reactive oxygen species on secretory component in the intestinal epithelium during hyperoxia. Experimental and Therapeutic Medicine, 14, 4033-4040. https://doi.org/10.3892/etm.2017.5027
MLA
Zhao, M., Tang, S., Xin, J., Liu, D."Influence of reactive oxygen species on secretory component in the intestinal epithelium during hyperoxia". Experimental and Therapeutic Medicine 14.5 (2017): 4033-4040.
Chicago
Zhao, M., Tang, S., Xin, J., Liu, D."Influence of reactive oxygen species on secretory component in the intestinal epithelium during hyperoxia". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4033-4040. https://doi.org/10.3892/etm.2017.5027
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao M, Tang S, Xin J and Liu D: Influence of reactive oxygen species on secretory component in the intestinal epithelium during hyperoxia. Exp Ther Med 14: 4033-4040, 2017.
APA
Zhao, M., Tang, S., Xin, J., & Liu, D. (2017). Influence of reactive oxygen species on secretory component in the intestinal epithelium during hyperoxia. Experimental and Therapeutic Medicine, 14, 4033-4040. https://doi.org/10.3892/etm.2017.5027
MLA
Zhao, M., Tang, S., Xin, J., Liu, D."Influence of reactive oxygen species on secretory component in the intestinal epithelium during hyperoxia". Experimental and Therapeutic Medicine 14.5 (2017): 4033-4040.
Chicago
Zhao, M., Tang, S., Xin, J., Liu, D."Influence of reactive oxygen species on secretory component in the intestinal epithelium during hyperoxia". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4033-4040. https://doi.org/10.3892/etm.2017.5027
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team