Open Access

Growth/differentiation factor‑5 promotes in vitro/vivo periodontal specific differentiation of induced pluripotent stem cell‑derived mesenchymal stem cells

  • Authors:
    • Xiaohui Yin
    • Peng Li
    • Yang Li
    • Yu Cai
    • Jinhua Wen
    • Qingxian Luan
  • View Affiliations

  • Published online on: August 25, 2017     https://doi.org/10.3892/etm.2017.5030
  • Pages: 4111-4117
  • Copyright: © Yin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Mesenchymal stem cells (MSCs) derived from induced pluripotent stem cells (iPSCs) represent a promising alternative source of MSCs for effective periodontal regeneration. Scientific evidence has demonstrated that growth/differentiation factor‑5 (GDF‑5) supports regeneration of periodontal tissues and has a key role in MSC differentiation. The present study investigated the effects of recombinant human GDF‑5 (rhGDF‑5) on periodontal specific differentiation of iPSC‑derived MSCs (iPSC‑MSCs) and bone marrow mesenchymal stem cells (BMSCs). rhGDF‑5 treatment in vitro significantly enhanced the expression levels of marker genes associated with osteogenesis (OCN), fibrogenesis (periostin) and cementogenesis (CAP) in the iPSC‑MSCs compared with untreated controls (all P<0.05). Interestingly, the rhGDF‑5‑treated BMSCs failed to exhibit overexpression of periostin and CAP despite highly upregulated expression of OCN. In the presence of rhGDF‑5, both the iPSC‑MSCs and BMSCs demonstrated marked formation of mineralized nodules. Notably, rhGDF‑5 greatly promoted periodontal specific differentiation of the iPSC‑MSCs encapsulated in hyaluronic acid (HA) hydrogels in vivo as determined by immunohistochemical and immunofluorescence staining. The majority of the PKH67‑labeled iPSC‑MSCs implanted with rhGDF‑5 exhibited strong expression of OCN, periostin and CAP. In conclusion, iPSC‑MSCs demonstrate high periodontal specific differentiation potential in response to rhGDF‑5 both in vitro and in vivo. The delivery of iPSC‑MSCs and rhGDF‑5 with HA hydrogel may have beneficial effects in regenerative periodontal therapy.
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November-2017
Volume 14 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Yin X, Li P, Li Y, Cai Y, Wen J and Luan Q: Growth/differentiation factor‑5 promotes in vitro/vivo periodontal specific differentiation of induced pluripotent stem cell‑derived mesenchymal stem cells. Exp Ther Med 14: 4111-4117, 2017
APA
Yin, X., Li, P., Li, Y., Cai, Y., Wen, J., & Luan, Q. (2017). Growth/differentiation factor‑5 promotes in vitro/vivo periodontal specific differentiation of induced pluripotent stem cell‑derived mesenchymal stem cells. Experimental and Therapeutic Medicine, 14, 4111-4117. https://doi.org/10.3892/etm.2017.5030
MLA
Yin, X., Li, P., Li, Y., Cai, Y., Wen, J., Luan, Q."Growth/differentiation factor‑5 promotes in vitro/vivo periodontal specific differentiation of induced pluripotent stem cell‑derived mesenchymal stem cells". Experimental and Therapeutic Medicine 14.5 (2017): 4111-4117.
Chicago
Yin, X., Li, P., Li, Y., Cai, Y., Wen, J., Luan, Q."Growth/differentiation factor‑5 promotes in vitro/vivo periodontal specific differentiation of induced pluripotent stem cell‑derived mesenchymal stem cells". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4111-4117. https://doi.org/10.3892/etm.2017.5030