Open Access

Magnetic resonance spectroscopy for assessment of brain injury in the rat model of sepsis

  • Authors:
    • Miaoyun Wen
    • Zhesi Lian
    • Linqiang Huang
    • Senzhi Zhu
    • Bei Hu
    • Yongli Han
    • Yiyu Deng
    • Hongke Zeng
  • View Affiliations

  • Published online on: August 25, 2017     https://doi.org/10.3892/etm.2017.5034
  • Pages: 4118-4124
  • Copyright: © Wen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The diagnostic value of magnetic resonance spectroscopy (MRS), T2-weighted imaging (T2WI) and serum markers of brain injury in a rat model of sepsis were investigated. Rats were randomly divided into the control group and 6, 12 and 24 h after lipopolysaccharide-injection groups. Brain morphology and metabolism were assessed with T2WI magnetic resonance imaging (MRI) and MRS. Serum and brain tissue samples were then collected to examine the concentrations of neuron‑specific enolase (NSE) and S100-β protein. Brain T2WI showed no differences between the groups. N-acetylaspartate/choline (NAA/Cr) ratio measured by MRS showed different degrees of decrease in the sepsis groups, and serum NSE and S100-β concentrations were increased compared with the control group. Apoptosis rates were measured in the right hippocampal area, and there were statistically significant differences between the indicated groups and the control group (p<0.05). The correlation between apoptosis rate and NAA/Cr ratio was closer than that between apoptosis rate and NSE or S100-β (-0.925 vs. 0.434 vs. 0.517, respectively). In conclusion, MRS is a sensitive, non-invasive method to investigate complications of brain injury in septic rats, which may be utilized for the early diagnosis of brain injury caused by sepsis.
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November-2017
Volume 14 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wen M, Lian Z, Huang L, Zhu S, Hu B, Han Y, Deng Y and Zeng H: Magnetic resonance spectroscopy for assessment of brain injury in the rat model of sepsis. Exp Ther Med 14: 4118-4124, 2017
APA
Wen, M., Lian, Z., Huang, L., Zhu, S., Hu, B., Han, Y. ... Zeng, H. (2017). Magnetic resonance spectroscopy for assessment of brain injury in the rat model of sepsis. Experimental and Therapeutic Medicine, 14, 4118-4124. https://doi.org/10.3892/etm.2017.5034
MLA
Wen, M., Lian, Z., Huang, L., Zhu, S., Hu, B., Han, Y., Deng, Y., Zeng, H."Magnetic resonance spectroscopy for assessment of brain injury in the rat model of sepsis". Experimental and Therapeutic Medicine 14.5 (2017): 4118-4124.
Chicago
Wen, M., Lian, Z., Huang, L., Zhu, S., Hu, B., Han, Y., Deng, Y., Zeng, H."Magnetic resonance spectroscopy for assessment of brain injury in the rat model of sepsis". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4118-4124. https://doi.org/10.3892/etm.2017.5034