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Article

Expression and significance of caveolin‑1 in hepatitis B virus‑associated hepatocellular carcinoma

  • Authors:
    • Hao Cheng
    • Yiming Pan
    • Yongzhong Yao
    • Zhanghua Zhu
    • Jun Chen
    • Xitai Sun
    • Yudong Qiu
    • Yitao Ding
  • View Affiliations / Copyright

    Affiliations: Department of Hepato‑Pancreato‑Biliary Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China, Department of Intensive Care Unit, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China, Department of Pathology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China
  • Pages: 4356-4362
    |
    Published online on: August 25, 2017
       https://doi.org/10.3892/etm.2017.5038
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Abstract

Caveolin‑1 (Cav‑1) is a major component of caveolae and has been recently identified as a tumor suppressor. As little is known about Cav‑1 in hepatitis B virus (HBV)‑associated hepatocellular carcinoma (HCC), the aim of the present study was to investigate the expression and significance of Cav‑1 in HBV‑associated HCC. Semi‑quantitative reverse transcription‑polymerase chain reaction (RT‑PCR) was performed to detect the mRNA expression level of Cav‑1 in 40 cases of HBV‑associated HCC, the corresponding 11 non‑tumor cases of HBV‑associated chronic hepatitis, 29 non‑tumor cases of HBV‑associated cirrhosis and 6 cases of normal liver tissues. Immunohistochemical analysis indicated the expression of Cav‑1, cluster of differentiation 34 and vascular endothelial growth factor (VEGF) in HBV‑associated HCC tissue samples. In addition, the association of Cav‑1 expression with angiogenesis and clinicopathological characteristics of HBV‑associated HCC was also analyzed. RT‑PCR results demonstrated that the expression rate of Cav‑1 mRNA in HBV‑associated HCC, non‑tumor HBV‑associated chronic hepatitis and cirrhosis liver tissues and control normal liver tissues from patients with metastatic carcinoma was 92.5, 85.0 and 16.7%, respectively. mRNA expression level of Cav‑1 was significantly increased in chronic hepatitis, cirrhosis and HBV‑associated HCC livers compared with normal control livers (P<0.05 and P<0.01, respectively). Cav‑1 protein was detected by immunohistochemistry in 80% of the samples of HBV‑associated HCC. Furthermore, Cav‑1 and VEGF protein expression levels were correlated with microvessel density (MVD; γs<0.46, P=0.01 and γs<0.31, P=0.05, respectively). In addition, Cav‑1 expression and MVD were significantly associated with metastasis (P=0.031 and P=0.046, respectively). In conclusion, Cav‑1 may have an important role in the carcinogenesis and progression of HBV‑associated HCC and angiogenesis may be affected by Cav‑1 during this process.
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Copy and paste a formatted citation
Spandidos Publications style
Cheng H, Pan Y, Yao Y, Zhu Z, Chen J, Sun X, Qiu Y and Ding Y: Expression and significance of caveolin‑1 in hepatitis B virus‑associated hepatocellular carcinoma. Exp Ther Med 14: 4356-4362, 2017.
APA
Cheng, H., Pan, Y., Yao, Y., Zhu, Z., Chen, J., Sun, X. ... Ding, Y. (2017). Expression and significance of caveolin‑1 in hepatitis B virus‑associated hepatocellular carcinoma. Experimental and Therapeutic Medicine, 14, 4356-4362. https://doi.org/10.3892/etm.2017.5038
MLA
Cheng, H., Pan, Y., Yao, Y., Zhu, Z., Chen, J., Sun, X., Qiu, Y., Ding, Y."Expression and significance of caveolin‑1 in hepatitis B virus‑associated hepatocellular carcinoma". Experimental and Therapeutic Medicine 14.5 (2017): 4356-4362.
Chicago
Cheng, H., Pan, Y., Yao, Y., Zhu, Z., Chen, J., Sun, X., Qiu, Y., Ding, Y."Expression and significance of caveolin‑1 in hepatitis B virus‑associated hepatocellular carcinoma". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4356-4362. https://doi.org/10.3892/etm.2017.5038
Copy and paste a formatted citation
x
Spandidos Publications style
Cheng H, Pan Y, Yao Y, Zhu Z, Chen J, Sun X, Qiu Y and Ding Y: Expression and significance of caveolin‑1 in hepatitis B virus‑associated hepatocellular carcinoma. Exp Ther Med 14: 4356-4362, 2017.
APA
Cheng, H., Pan, Y., Yao, Y., Zhu, Z., Chen, J., Sun, X. ... Ding, Y. (2017). Expression and significance of caveolin‑1 in hepatitis B virus‑associated hepatocellular carcinoma. Experimental and Therapeutic Medicine, 14, 4356-4362. https://doi.org/10.3892/etm.2017.5038
MLA
Cheng, H., Pan, Y., Yao, Y., Zhu, Z., Chen, J., Sun, X., Qiu, Y., Ding, Y."Expression and significance of caveolin‑1 in hepatitis B virus‑associated hepatocellular carcinoma". Experimental and Therapeutic Medicine 14.5 (2017): 4356-4362.
Chicago
Cheng, H., Pan, Y., Yao, Y., Zhu, Z., Chen, J., Sun, X., Qiu, Y., Ding, Y."Expression and significance of caveolin‑1 in hepatitis B virus‑associated hepatocellular carcinoma". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4356-4362. https://doi.org/10.3892/etm.2017.5038
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