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Article

Long non‑coding RNA HOX transcript antisense RNA promotes expression of 14‑3‑3σ in non‑small cell lung cancer

  • Authors:
    • Ranran Wang
    • Bin Yan
    • Zheng Li
    • Yiqun Jiang
    • Chao Mao
    • Xiang Wang
    • Xinmin Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic and Cardiovascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China, Cancer Research Institute, Central South University, Changsha, Hunan 410011, P.R. China
  • Pages: 4503-4508
    |
    Published online on: August 28, 2017
       https://doi.org/10.3892/etm.2017.5041
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Abstract

Evidence suggests that both 14‑3‑3σ and long non‑coding RNA HOX transcript antisense RNA (HOTAIR) are involved in the tumorigenesis and progression of lung cancer. In the present study, the potential association between 14‑3‑3σ and HOTAIR in non‑small cell lung cancer (NSCLC) was investigated. In tissue samples collected from 54 patients with NSCLC, expression of HOTAIR and 14‑3‑3σ was analyzed by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). After stable ectopic expression of HOTAIR and stable HOTAIR knockdown in PC9 cancer cells, the effect of HOTAIR on levels of mRNA and protein 14‑3‑3σ expression levels were detected using RT‑qPCR and western blotting, respectively. Expression of HOTAIR and 14‑3‑3σ in NSCLC tissues was significantly higher than in adjacent non‑cancerous lung tissue (P<0.05). Correlation analysis also identified a correlation between levels of HOTAIR and 14‑3‑3σ expression in NSCLC tissues (r=0.725, P=0.0005). In addition, overexpression and knockdown of HOTAIR in the human NSCLC cell line PC9 led to the upregulation and downregulation of 14‑3‑3σ, respectively, at both the mRNA and protein levels (all P<0.05). To the best of our knowledge, the present study provides the first in vivo and in vitro evidence to suggest that HOTAIR promotes the expression of 14‑3‑3σ in NSCLC. The potential association between HOTAIR and 14‑3‑3σ indicates that both biomolecules may be viable targets in anticancer therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Wang R, Yan B, Li Z, Jiang Y, Mao C, Wang X and Zhou X: Long non‑coding RNA HOX transcript antisense RNA promotes expression of 14‑3‑3σ in non‑small cell lung cancer. Exp Ther Med 14: 4503-4508, 2017.
APA
Wang, R., Yan, B., Li, Z., Jiang, Y., Mao, C., Wang, X., & Zhou, X. (2017). Long non‑coding RNA HOX transcript antisense RNA promotes expression of 14‑3‑3σ in non‑small cell lung cancer. Experimental and Therapeutic Medicine, 14, 4503-4508. https://doi.org/10.3892/etm.2017.5041
MLA
Wang, R., Yan, B., Li, Z., Jiang, Y., Mao, C., Wang, X., Zhou, X."Long non‑coding RNA HOX transcript antisense RNA promotes expression of 14‑3‑3σ in non‑small cell lung cancer". Experimental and Therapeutic Medicine 14.5 (2017): 4503-4508.
Chicago
Wang, R., Yan, B., Li, Z., Jiang, Y., Mao, C., Wang, X., Zhou, X."Long non‑coding RNA HOX transcript antisense RNA promotes expression of 14‑3‑3σ in non‑small cell lung cancer". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4503-4508. https://doi.org/10.3892/etm.2017.5041
Copy and paste a formatted citation
x
Spandidos Publications style
Wang R, Yan B, Li Z, Jiang Y, Mao C, Wang X and Zhou X: Long non‑coding RNA HOX transcript antisense RNA promotes expression of 14‑3‑3σ in non‑small cell lung cancer. Exp Ther Med 14: 4503-4508, 2017.
APA
Wang, R., Yan, B., Li, Z., Jiang, Y., Mao, C., Wang, X., & Zhou, X. (2017). Long non‑coding RNA HOX transcript antisense RNA promotes expression of 14‑3‑3σ in non‑small cell lung cancer. Experimental and Therapeutic Medicine, 14, 4503-4508. https://doi.org/10.3892/etm.2017.5041
MLA
Wang, R., Yan, B., Li, Z., Jiang, Y., Mao, C., Wang, X., Zhou, X."Long non‑coding RNA HOX transcript antisense RNA promotes expression of 14‑3‑3σ in non‑small cell lung cancer". Experimental and Therapeutic Medicine 14.5 (2017): 4503-4508.
Chicago
Wang, R., Yan, B., Li, Z., Jiang, Y., Mao, C., Wang, X., Zhou, X."Long non‑coding RNA HOX transcript antisense RNA promotes expression of 14‑3‑3σ in non‑small cell lung cancer". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4503-4508. https://doi.org/10.3892/etm.2017.5041
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