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The protective effects of ischemic preconditioning on rats with renal ischemia-reperfusion injury and the effects on the expression of Bcl-2 and Bax

  • Authors:
    • Sheng Shen
    • Jiexue Zhou
    • Shandong Meng
    • Jiaqing Wu
    • Juan Ma
    • Chunli Zhu
    • Gengguo Deng
    • Dong Liu
  • View Affiliations / Copyright

    Affiliations: Department of Organ Transplantation, Guangdong Second Provincial General Hospital, Guangzhou, Guangdong 510317, P.R. China
    Copyright: © Shen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4077-4082
    |
    Published online on: August 28, 2017
       https://doi.org/10.3892/etm.2017.5047
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Abstract

The aim of the present study was to investigate the protective effects of ischemic preconditioning on rats with renal ischemia-reperfusion injury and the effects on the expression of Bcl-2 and Bax. Thirty-six SD rats were randomly divided into three groups (n=12) including sham operation (S) group, ischemia-reperfusion group (I/R) group and ischemic preconditioning (IP) group. After anesthesia with intraperitoneal injection of chloral hydrate, bilateral renal pedicles were clipped for 45 min, followed by perfusion for 6 h to establish the I/R model. Both kidneys in rats of S group were separated and exposed for 45 min, but renal pedicles were not clipped. In IP group, bilateral renal pedicles were clipped for 5 min, followed by perfusion for 5 min, this procedure was repeated 3 times. Then bilateral renal pedicles were clipped for 45 min, followed by perfusion for 6 h. Blood samples were collected and rats were sacrificed to collect renal tissue. Levels of serum creatinine (Cr) and blood urea nitrogen (BUN) were measured. Activity of superoxide dismutase (SOD) was measured by xanthine oxidase assay. Degree of renal injury was evaluated by H&E staining. TUNEL kit was used to detect the number of apoptotic cells in renal tissue. Expression levels of Bcl-2 and Bax were detected by semi-quantitative PCR and western blot analysis at mRNA and protein levels, respectively. Results showed that levels of Cr and BUN in I/R and IP groups were significantly higher than those in S group, and levels of Cr and BUN in I/R group were significantly higher than that in IP group (p<0.05). Activity of SOD in I/R group and IP group were significantly lower than those in S group, and activity of SOD in I/R group were significantly lower than those in IP group (p<0.05). H&E staining showed that, compared with S group, renal injury in the I/R and IP groups was more serious than that in the S group, and I/R group was more serious than the IP group (p<0.05). TUNEL apoptosis assay showed that number of apoptotic cells in IP and I/R groups were significantly higher than that in the S group (p<0.01). Semi-quantitative PCR and western blot analysis showed that, compared with the S group, expression levels of Bcl-2 mRNA and protein were significantly decreased, expression levels of Bax mRNA and protein were significantly increased, and the ratio of Bcl-2/Bax was significantly decreased in the IP and I/R groups (p<0.01). Compared with the I/R group, expression level of Bcl-2 was significantly increased, the level of Bax was significantly deceased, and the ratio of Bcl-2/Bax was significantly increased in the IP group (p<0.01). As a result, ischemic preconditioning can protect rats with renal ischemia-reperfusion injury possibly by increasing the expression level of Bcl-2 and decreasing the expression level of Bax.
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View References

1 

Kim Y, Ge Y, Tao C, Zhu J, Chapman AB, Torres VE, Yu AS, Mrug M, Bennett WM, Flessner MF, et al: Consortium for Radiologic Imaging Studies of Polycystic Kidney Disease (CRISP): Automated segmentation of kidneys from MR images in patients with autosomal dominant polycystic kidney disease. Clin J Am Soc Nephrol. 11:576–584. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Fox CS, Matsushita K, Woodward M, Bilo HJ, Chalmers J, Heerspink HJ, Lee BJ, Perkins RM, Rossing P, Sairenchi T, et al: Chronic Kidney Disease Prognosis Consortium: Associations of kidney disease measures with mortality and end-stage renal disease in individuals with and without diabetes: A meta-analysis. Lancet. 380:1662–1673. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Haynes R, Staplin N, Emberson J, Herrington WG, Tomson C, Agodoa L, Tesar V, Levin A, Lewis D, Reith C, et al: SHARP Collaborative Group: Evaluating the contribution of the cause of kidney disease to prognosis in CKD: Results from the Study of Heart and Renal Protection (SHARP). Am J Kidney Dis. 64:40–48. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Ta MH, Schwensen KG, Foster S, Korgaonkar M, Ozimek-Kulik JE, Phillips JK, Peduto A and Rangan GK: Effects of TORC1 inhibition during the early and established phases of polycystic kidney disease. PLoS One. 11:e01641932016. View Article : Google Scholar : PubMed/NCBI

5 

Takahashi-Sato K, Murakawa M, Kimura J, Ito MA and Matsuoka I: Loss of ectonucleotidases from the coronary vascular bed after ischemia-reperfusion in isolated rat heart. BMC Cardiovasc Disord. 68:2577–2584. 2016.

6 

Chua S, Lee FY, Tsai TH, Sheu JJ, Leu S, Sun CK, Chen YL, Chang HW, Chai HT, Liu C, et al: Inhibition of dipeptidyl peptidase-IV enzyme activity protects against myocardial ischemia-reperfusion injury in rats. J Transl Med. 19:285–297. 2016.

7 

Wang K, Zhang J, Liu J, Tian J, Wu Y, Wang X, Quan L, Xu H, Wang W and Liu H: Variations in the protein level of Omi/HtrA2 in the heart of aged rats may contribute to the increased susceptibility of cardiomyocytes to ischemia/reperfusion injury and cell death: Omi/HtrA2 and aged heart injury. Age (Dordr). 30:266–271. 2016.

8 

Huang P, Sun Y, Yang J, Chen S, Liu AD, Holmberg L, Huang X, Tang C, Du J and Jin H: The ERK1/2 signaling pathway is involved in sulfur dioxide preconditioning-induced protection against cardiac dysfunction in isolated perfused rat heart subjected to myocardial ischemia/reperfusion. Int J Mol Sci. 206:26–32. 2016.

9 

ONeill KL, Huang K, Zhang J, Chen Y and Luo X: Inactivation of prosurvival Bcl-2 proteins activates Bax/Bak through the outer mitochondrial membrane. Genes Dev. 30:973–988. 2016. View Article : Google Scholar : PubMed/NCBI

10 

Lindqvist LM, Heinlein M, Huang DC and Vaux DL: Prosurvival Bcl-2 family members affect autophagy only indirectly, by inhibiting Bax and Bak. Invest New Drugs. 34:713–725. 2016.

11 

Zhang WZ, Li R, Liu S, Zhang JD, Ning XF and Cai SL: Effects of renal ischemic postconditioning on myocardial ultrastructural organization and myocardial expression of Bcl-2/Bax in rabbits. BioMed Res Int. 2016:93494372016. View Article : Google Scholar : PubMed/NCBI

12 

Shen S, Wang JF, Wu JQ, Zhou JX, Meng SD, Ma J, Zhu CL, Deng GG and Liu D: GC/MS-based metabolomic analysis of alleviated renal ischemia-reperfusion injury induced by remote ischemic preconditioning. Eur Rev Med Pharmacol Sci. 21:765–774. 2017.PubMed/NCBI

13 

Tawfik MK: Renoprotective activity of telmisartan versus pioglitazone on ischemia/reperfusion induced renal damage in diabetic rats. Eur Rev Med Pharmacol Sci. 16:600–609. 2012.PubMed/NCBI

14 

Park SY, Shrestha S, Youn YJ, Kim JK and Kim SY: Role of Toll-like receptor-4 in renal graft ischemia-reperfusion injury. Asian Pac J Trop Med. 9:2–9. 2016.

15 

Kierulf-Lassen C, Kristensen ML, Birn H, Jespersen B and Nørregaard R: No effect of remote ischemic conditioning strategies on recovery from renal ischemia-reperfusion injury and protective molecular mediators. BMC Cancer. 16:686–691. 2016.PubMed/NCBI

16 

Chen YT, Tsai TH, Yang CC, Sun CK, Chang LT, Chen HH, Chang CL, Sung PH, Zhen YY, Leu S, et al: Exendin-4 and sitagliptin protect kidney from ischemia-reperfusion injury through suppressing oxidative stress and inflammatory reaction. J Transl Med. 11:2702013. View Article : Google Scholar : PubMed/NCBI

17 

Li Y and Liu S: The effect of dexmedetomidine on oxidative stress response following cerebral ischemia-reperfusion in rats and the expression of intracellular adhesion molecule-1 (ICAM-1) and S100B. Med Sci Monit. 23:867–873. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Batinic-Haberle I, Tovmasyan A, Roberts ER, Vujaskovic Z, Leong KW and Spasojevic I: SOD therapeutics: Latest insights into their structure-activity relationships and impact on the cellular redox-based signaling pathways. Antioxid Redox Signal. 20:2372–2415. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Zhang L, Gan W and An G: Influence of Tanshinone IIa on heat shock protein 70, Bcl-2 and Bax expression in rats with spinal ischemia/reperfusion injury. Neural Regen Res. 7:2882–2888. 2012.PubMed/NCBI

20 

Ma Z, Wei Q, Dong G, Huo Y and Dong Z: DNA damage response in renal ischemia-reperfusion and ATP-depletion injury of renal tubular cells. Biochim Biophys Acta. 1842:1088–1096. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Shen S, Zhou J, Meng S, Wu J, Ma J, Zhu C, Deng G and Liu D: The protective effects of ischemic preconditioning on rats with renal ischemia-reperfusion injury and the effects on the expression of Bcl-2 and Bax. Exp Ther Med 14: 4077-4082, 2017.
APA
Shen, S., Zhou, J., Meng, S., Wu, J., Ma, J., Zhu, C. ... Liu, D. (2017). The protective effects of ischemic preconditioning on rats with renal ischemia-reperfusion injury and the effects on the expression of Bcl-2 and Bax. Experimental and Therapeutic Medicine, 14, 4077-4082. https://doi.org/10.3892/etm.2017.5047
MLA
Shen, S., Zhou, J., Meng, S., Wu, J., Ma, J., Zhu, C., Deng, G., Liu, D."The protective effects of ischemic preconditioning on rats with renal ischemia-reperfusion injury and the effects on the expression of Bcl-2 and Bax". Experimental and Therapeutic Medicine 14.5 (2017): 4077-4082.
Chicago
Shen, S., Zhou, J., Meng, S., Wu, J., Ma, J., Zhu, C., Deng, G., Liu, D."The protective effects of ischemic preconditioning on rats with renal ischemia-reperfusion injury and the effects on the expression of Bcl-2 and Bax". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4077-4082. https://doi.org/10.3892/etm.2017.5047
Copy and paste a formatted citation
x
Spandidos Publications style
Shen S, Zhou J, Meng S, Wu J, Ma J, Zhu C, Deng G and Liu D: The protective effects of ischemic preconditioning on rats with renal ischemia-reperfusion injury and the effects on the expression of Bcl-2 and Bax. Exp Ther Med 14: 4077-4082, 2017.
APA
Shen, S., Zhou, J., Meng, S., Wu, J., Ma, J., Zhu, C. ... Liu, D. (2017). The protective effects of ischemic preconditioning on rats with renal ischemia-reperfusion injury and the effects on the expression of Bcl-2 and Bax. Experimental and Therapeutic Medicine, 14, 4077-4082. https://doi.org/10.3892/etm.2017.5047
MLA
Shen, S., Zhou, J., Meng, S., Wu, J., Ma, J., Zhu, C., Deng, G., Liu, D."The protective effects of ischemic preconditioning on rats with renal ischemia-reperfusion injury and the effects on the expression of Bcl-2 and Bax". Experimental and Therapeutic Medicine 14.5 (2017): 4077-4082.
Chicago
Shen, S., Zhou, J., Meng, S., Wu, J., Ma, J., Zhu, C., Deng, G., Liu, D."The protective effects of ischemic preconditioning on rats with renal ischemia-reperfusion injury and the effects on the expression of Bcl-2 and Bax". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4077-4082. https://doi.org/10.3892/etm.2017.5047
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