Bio‑Oss® modified by calcitonin gene‑related peptide promotes osteogenesis in vitro

Li,Yuanjing; Yang,Lan; Zheng,Zhichao; Li,Zhengmao; Deng,Tian; Ren,Wen; Wu,Caijuan; Guo,Lvhua

doi:10.3892/etm.2017.5048

Bio‑Oss® modified by calcitonin gene‑related peptide promotes osteogenesis in vitro

Authors:
- Yuanjing Li
- Lan Yang
- Zhichao Zheng
- Zhengmao Li
- Tian Deng
- Wen Ren
- Caijuan Wu
- Lvhua Guo
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Affiliations: Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510140, P.R. China
Published online on: August 28, 2017 https://doi.org/10.3892/etm.2017.5048
Pages: 4001-4008
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Bio‑Oss® and α‑calcitonin gene‑related peptide (CGRP) are involved in osteogenesis. However, it has remained to be assessed how α‑CGRP affects the effect of Bio‑Oss. In the present study, primary osteoblasts were incubated with α‑CGRP, Bio‑Oss, α‑GGRP‑Bio‑Oss or mimic‑α‑CGRP. The proliferation rate, mineralization nodules, alkaline phosphatase (ALP) activity and the expression of osteogenic genes were measured by a Cell Counting Kit‑8 assay, Alizarin Red‑S staining, ALP activity detection and reverse‑transcription quantitative PCR as well as western blot analysis, respectively. The proliferation rate, ALP activity and the number of mineralization nodules were significantly increased in the α‑CGRP‑modified Bio‑Oss group compared to that in the Bio‑Oss group. The mRNA and protein levels of osteocalcin, Runt‑related transcription factor‑2 and ALP were significantly upregulated in the α‑CGRP‑Bio‑Oss group compared with those in the Bio‑Oss group. Furthermore, the effect of mimic‑α‑CGRP on osteogenesis was reduced as it carried a mutation. In conclusion, the present study was the first to demonstrate that Bio‑Oss modified with CGRP contributed to osteogenesis and may provide a novel formulation applied in the clinic for restoration of large bone defects.

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