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Bio‑Oss® modified by calcitonin gene‑related peptide promotes osteogenesis in vitro

  • Authors:
    • Yuanjing Li
    • Lan Yang
    • Zhichao Zheng
    • Zhengmao Li
    • Tian Deng
    • Wen Ren
    • Caijuan Wu
    • Lvhua Guo
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510140, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4001-4008
    |
    Published online on: August 28, 2017
       https://doi.org/10.3892/etm.2017.5048
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Abstract

Bio‑Oss® and α‑calcitonin gene‑related peptide (CGRP) are involved in osteogenesis. However, it has remained to be assessed how α‑CGRP affects the effect of Bio‑Oss. In the present study, primary osteoblasts were incubated with α‑CGRP, Bio‑Oss, α‑GGRP‑Bio‑Oss or mimic‑α‑CGRP. The proliferation rate, mineralization nodules, alkaline phosphatase (ALP) activity and the expression of osteogenic genes were measured by a Cell Counting Kit‑8 assay, Alizarin Red‑S staining, ALP activity detection and reverse‑transcription quantitative PCR as well as western blot analysis, respectively. The proliferation rate, ALP activity and the number of mineralization nodules were significantly increased in the α‑CGRP‑modified Bio‑Oss group compared to that in the Bio‑Oss group. The mRNA and protein levels of osteocalcin, Runt‑related transcription factor‑2 and ALP were significantly upregulated in the α‑CGRP‑Bio‑Oss group compared with those in the Bio‑Oss group. Furthermore, the effect of mimic‑α‑CGRP on osteogenesis was reduced as it carried a mutation. In conclusion, the present study was the first to demonstrate that Bio‑Oss modified with CGRP contributed to osteogenesis and may provide a novel formulation applied in the clinic for restoration of large bone defects.
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1 

Younger EM and Chapman MW: Morbidity at bone graft donor sites. J Orthop Trauma. 3:192–195. 1989. View Article : Google Scholar : PubMed/NCBI

2 

Milinković ZB, Krneta O, Milicković S, Dozić D and Curcić A: Are the additional grafts necessary? Acta Chir Iugosl. 57:69–72. 2010. View Article : Google Scholar

3 

Myeroff C and Archdeacon M: Autogenous bone graft: Donor sites and techniques. J Bone Joint Surg Am. 93:2227–2236. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Tapety FI, Amizuka N, Uoshima K, Nomura S and Maeda T: A histological evaluation of the involvement of Bio-Oss in osteoblastic differentiation and matrix synthesis. Clin Oral Implants Res. 15:315–324. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Schlegel AK: Bio-Oss bone replacement material. Long-term results with Bio-Oss bone replacement material. Schweiz Monatsschr Zahnmed. 106:141–149. 1996.(In French, German). PubMed/NCBI

6 

Schlegel AK and Donath K: BIO-OSS-a resorbable bone substitute? J Long Term Eff Med Implants. 8:201–209. 1998.PubMed/NCBI

7 

Fulmer NL, Bussard GM, Gampper TJ and Edlich RF: Anorganic bovine bone and analogs of bone mineral as implants for craniofacial surgery: A literature review. J Long Term Eff Med Implants. 8:69–78. 1998.PubMed/NCBI

8 

Amerio P, Vianale G, Reale M, Muraro R, Tulli A and Piattelli A: The effect of deproteinized bovine bone on osteoblast growth factors and proinflammatory cytokine production. Clin Oral Implants Res. 21:650–655. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Diener HC: CGRP as a new target in prevention and treatment of migraine. Lancet Neurol. 13:1065–1067. 2014. View Article : Google Scholar : PubMed/NCBI

10 

de Jong PR, Takahashi N, Peiris M, Bertin S, Lee J, Gareau MG, Paniagua A, Harris AR, Herdman DS and Corr M: TRPM8 on mucosal sensory nerves regulates colitogenic responses by innate immune cells via CGRP. Mucosal Immunol. 8:491–504. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Sample SJ, Hao Z, Wilson AP and Muir P: Role of calcitonin gene-related peptide in bone repair after cyclic fatigue loading. PLoS One. 6:e203862011. View Article : Google Scholar : PubMed/NCBI

12 

Hirt D and Bernard GW: CGRP-beta unlike CGRP-alpha has no osteogenic stimulatory effect in vitro. Peptides. 18:1461–1463. 1997. View Article : Google Scholar : PubMed/NCBI

13 

Han N, Zhang DY, Wang TB, Zhang PX and Jiang BG: Calcitonin gene-related peptide induces proliferation and monocyte chemoattractant protein-1 expression via extracellular signal-regulated kinase activation in rat osteoblasts. Chin Med J (Engl). 123:1748–1753. 2010.PubMed/NCBI

14 

Mrak E, Guidobono F, Moro G, Fraschini G, Rubinacci A and Villa I: Calcitonin gene-related peptide (CGRP) inhibits apoptosis in human osteoblasts by β-catenin stabilization. J Cell Physiol. 225:701–708. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Ballica R, Valentijn K, Khachatryan A, Guerder S, Kapadia S, Gundberg C, Gilligan J, Flavell RA and Vignery A: Targeted expression of calcitonin gene-related peptide to osteoblasts increases bone density in mice. J Bone Miner Res. 14:1067–6574. 1999. View Article : Google Scholar : PubMed/NCBI

16 

Schinke T, Liese S, Priemel M, Haberland M, Schilling AF, Catala-Lehnen P, Blicharski D, Rueger JM, Gagel RF, Emeson RB and Amling M: Decreased bone formation and osteopenia in mice lacking alpha-calcitonin gene-related peptide. J Bone Miner Res. 19:2049–2056. 2004. View Article : Google Scholar : PubMed/NCBI

17 

Valentijn K, Gutow AP, Troiano N, Gundberg C, Gilligan JP and Vignery A: Effects of calcitonin gene-related peptide on bone turnover in ovariectomized rats. Bone. 21:269–274. 1997. View Article : Google Scholar : PubMed/NCBI

18 

Han N, Zhang DY, Wang TB, Zhang PX and Jiang BG: Calcitonin gene-related peptide induces proliferation and monocyte chemoattractant protein-1 expression via extracellular signal-regulated kinase activation in rat osteoblasts. Chin Med J (Engl). 123:1748–1753. 2010.PubMed/NCBI

19 

Shih C and Bernard GW: Calcitonin gene related peptide enhances bone colony development in vitro. Clin Orthop Relat Res. 335–344. 1997.PubMed/NCBI

20 

Xiao H, Huang C, Feng Y and Li R: Research situation and development of vacuum freeze-drying technology. Chin Med Equip J. 13:30–32. 2010.

21 

Schek RM, Wilke EN, Hollister SJ and Krebsbach PH: Combined use of designed scaffolds and adenoviral gene therapy for skeletal tissue engineering. Biomaterials. 27:1160–1166. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Jiang XQ, Chen JG, Gittens S, Chen CJ, Zhang XL and Zhang ZY: The ectopic study of tissue-engineered bone with hBMP-4 gene modified bone marrow stromal cells in rabbits. Chin Med J (Engl). 118:281–288. 2005.PubMed/NCBI

23 

Roldán JC, Detsch R, Schaefer S, Chang E, Kelantan M, Waiss W, Reichert TE, Gurtner GC and Deisinger U: Bone formation and degradation of a highly porous biphasic calcium phosphate ceramic in presence of BMP-7, VEGF and mesenchymal stem cells in an ectopic mouse model. J Craniomaxillofac Surg. 38:423–430. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Durham PL: Inhibition of calcitonin gene-related peptide function: A promising strategy for treating migraine. Headache. 48:1269–1275. 2008. View Article : Google Scholar : PubMed/NCBI

25 

Watkins HA, Au M and Hay DL: The structure of secretin family GPCR peptide ligands: Implications for receptor pharmacology and drug development. Drug Discov Today. 17:1006–1014. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Qi T, Christopoulos G, Bailey RJ, Christopoulos A, Sexton PM and Hay DL: Identification of N-terminal receptor activity-modifying protein residues important for calcitonin gene-related peptide, adrenomedullin, and amylin receptor function. Mol Pharmacol. 74:1059–1071. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Watkins HA, Rathbone DL, Barwell J, Hay DL and Poyner DR: Structure-activity relationships for α-calcitonin gene-related peptide. Br J Pharmacol. 170:1308–1322. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Chiba T, Yamaguchi A, Yamatani T, Nakamura A, Morishita T, Inui T, Fukase M, Noda T and Fujita T: Calcitonin gene-related peptide receptor antagonist human CGRP-(8–37). Am J Physiol. 256:E331–E335. 1989.PubMed/NCBI

29 

Dennis T, Fournier A, Cadieux A, Pomerleau F, Jolicoeur FB, St Pierre S and Quirion R: hCGRP8-37, a calcitonin gene-related peptide antagonist revealing calcitonin gene-related peptide receptor heterogeneity in brain and periphery. J Pharmacol Exp Ther. 254:123–128. 1990.PubMed/NCBI

30 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

31 

Mendonça G, Mendonça DB, Simões LG, Araújo AL, Leite ER, Duarte WR, Aragão FJ and Cooper LF: The effects of implant surface nanoscale features on osteoblast-specific gene expression. Biomaterials. 30:4053–4062. 2009. View Article : Google Scholar : PubMed/NCBI

32 

Wang L, Shi X, Zhao R, Halloran BP, Clark DJ, Jacobs CR and Kingery WS: Calcitonin gene-related peptide stimulates stromal cell osteogenic differentiation and inhibits RANKL induced NF-kappaB activation, osteoclastogenesis and bone resorption. Bone. 46:1369–1379. 2010. View Article : Google Scholar : PubMed/NCBI

33 

Cornish J, Callon KE, Bava U, Kamona SA, Cooper GJ and Reid IR: Effects of calcitonin, amylin, and calcitonin gene-related peptide on osteoclast development. Bone. 29:162–168. 2001. View Article : Google Scholar : PubMed/NCBI

34 

Turksen K, Bhargava U, Moe HK and Aubin JE: Isolation of monoclonal antibodies recognizing rat bone-associated molecules in vitro and in vivo. J Histochem Cytochem. 40:1339–1352. 1992. View Article : Google Scholar : PubMed/NCBI

35 

van den Beucken JJ, Walboomers XF, Boerman OC, Vos MR, Sommerdijk NA, Hayakawa T, Fukushima T, Okahata Y, Nolte RJ and Jansen JA: Functionalization of multilayered DNA-coatings with bone morphogenetic protein 2. J Control Release. 113:63–72. 2006. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li Y, Yang L, Zheng Z, Li Z, Deng T, Ren W, Wu C and Guo L: Bio‑Oss® modified by calcitonin gene‑related peptide promotes osteogenesis in vitro. Exp Ther Med 14: 4001-4008, 2017.
APA
Li, Y., Yang, L., Zheng, Z., Li, Z., Deng, T., Ren, W. ... Guo, L. (2017). Bio‑Oss® modified by calcitonin gene‑related peptide promotes osteogenesis in vitro. Experimental and Therapeutic Medicine, 14, 4001-4008. https://doi.org/10.3892/etm.2017.5048
MLA
Li, Y., Yang, L., Zheng, Z., Li, Z., Deng, T., Ren, W., Wu, C., Guo, L."Bio‑Oss® modified by calcitonin gene‑related peptide promotes osteogenesis in vitro". Experimental and Therapeutic Medicine 14.5 (2017): 4001-4008.
Chicago
Li, Y., Yang, L., Zheng, Z., Li, Z., Deng, T., Ren, W., Wu, C., Guo, L."Bio‑Oss® modified by calcitonin gene‑related peptide promotes osteogenesis in vitro". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4001-4008. https://doi.org/10.3892/etm.2017.5048
Copy and paste a formatted citation
x
Spandidos Publications style
Li Y, Yang L, Zheng Z, Li Z, Deng T, Ren W, Wu C and Guo L: Bio‑Oss® modified by calcitonin gene‑related peptide promotes osteogenesis in vitro. Exp Ther Med 14: 4001-4008, 2017.
APA
Li, Y., Yang, L., Zheng, Z., Li, Z., Deng, T., Ren, W. ... Guo, L. (2017). Bio‑Oss® modified by calcitonin gene‑related peptide promotes osteogenesis in vitro. Experimental and Therapeutic Medicine, 14, 4001-4008. https://doi.org/10.3892/etm.2017.5048
MLA
Li, Y., Yang, L., Zheng, Z., Li, Z., Deng, T., Ren, W., Wu, C., Guo, L."Bio‑Oss® modified by calcitonin gene‑related peptide promotes osteogenesis in vitro". Experimental and Therapeutic Medicine 14.5 (2017): 4001-4008.
Chicago
Li, Y., Yang, L., Zheng, Z., Li, Z., Deng, T., Ren, W., Wu, C., Guo, L."Bio‑Oss® modified by calcitonin gene‑related peptide promotes osteogenesis in vitro". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4001-4008. https://doi.org/10.3892/etm.2017.5048
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