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Highly pathogenic avian influenza A virus H5N1 non‑structural protein 1 is associated with apoptotic activation of the intrinsic mitochondrial pathway

  • Authors:
    • Qian Bian
    • Jing Lu
    • Li Zhang
    • Ying Chi
    • Yan Li
    • Hongxiong Guo
  • View Affiliations / Copyright

    Affiliations: Department of Toxicology and Function Assessment, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, Jiangsu 210009, P.R. China
    Copyright: © Bian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4041-4046
    |
    Published online on: August 28, 2017
       https://doi.org/10.3892/etm.2017.5056
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Abstract

Outbreaks of avian influenza A (H5N1) virus infection have significant health and economic consequences. Non‑structural protein 1 (NS1) is an essential virulence factor of the highly pathogenic H5N1 avian influenza virus and of the apoptosis associated with the pathogenesis of H5N1. Previous studies have revealed that the NS1 protein is able to induce apoptosis via an extrinsic pathway. However, it remains unclear whether the intrinsic pathway is also associated with this apoptosis. The present study used a clone of the NS1 gene from avian influenza A/Jiangsu/1/2007 and observed the localization of the NS1 protein and cytochrome c release from mitochondria and the change of mitochondrial membrane potential (MMP) in lung cancer cells. Cytotoxicity was detected using an MTT assay and the number of apoptotic cells was counted using a flow cytometer. Following the isolation of mitochondria, western blotting was performed to compare cytochrome c release from the mitochondria in cells before and after apoptosis. The change of MMP was detected using JC‑1 staining. Furthermore, the results reveal that the majority of the NS1 protein was localized in the cell nucleus, and that it may induce apoptosis of human lung epithelial cells. The apoptosis occurred with marked cytochrome c release from mitochondria and a change of the MMP. This indicated that the NS1 protein may be associated with apoptosis induced by an intrinsic mitochondrial pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Bian Q, Lu J, Zhang L, Chi Y, Li Y and Guo H: Highly pathogenic avian influenza A virus H5N1 non‑structural protein 1 is associated with apoptotic activation of the intrinsic mitochondrial pathway. Exp Ther Med 14: 4041-4046, 2017.
APA
Bian, Q., Lu, J., Zhang, L., Chi, Y., Li, Y., & Guo, H. (2017). Highly pathogenic avian influenza A virus H5N1 non‑structural protein 1 is associated with apoptotic activation of the intrinsic mitochondrial pathway. Experimental and Therapeutic Medicine, 14, 4041-4046. https://doi.org/10.3892/etm.2017.5056
MLA
Bian, Q., Lu, J., Zhang, L., Chi, Y., Li, Y., Guo, H."Highly pathogenic avian influenza A virus H5N1 non‑structural protein 1 is associated with apoptotic activation of the intrinsic mitochondrial pathway". Experimental and Therapeutic Medicine 14.5 (2017): 4041-4046.
Chicago
Bian, Q., Lu, J., Zhang, L., Chi, Y., Li, Y., Guo, H."Highly pathogenic avian influenza A virus H5N1 non‑structural protein 1 is associated with apoptotic activation of the intrinsic mitochondrial pathway". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4041-4046. https://doi.org/10.3892/etm.2017.5056
Copy and paste a formatted citation
x
Spandidos Publications style
Bian Q, Lu J, Zhang L, Chi Y, Li Y and Guo H: Highly pathogenic avian influenza A virus H5N1 non‑structural protein 1 is associated with apoptotic activation of the intrinsic mitochondrial pathway. Exp Ther Med 14: 4041-4046, 2017.
APA
Bian, Q., Lu, J., Zhang, L., Chi, Y., Li, Y., & Guo, H. (2017). Highly pathogenic avian influenza A virus H5N1 non‑structural protein 1 is associated with apoptotic activation of the intrinsic mitochondrial pathway. Experimental and Therapeutic Medicine, 14, 4041-4046. https://doi.org/10.3892/etm.2017.5056
MLA
Bian, Q., Lu, J., Zhang, L., Chi, Y., Li, Y., Guo, H."Highly pathogenic avian influenza A virus H5N1 non‑structural protein 1 is associated with apoptotic activation of the intrinsic mitochondrial pathway". Experimental and Therapeutic Medicine 14.5 (2017): 4041-4046.
Chicago
Bian, Q., Lu, J., Zhang, L., Chi, Y., Li, Y., Guo, H."Highly pathogenic avian influenza A virus H5N1 non‑structural protein 1 is associated with apoptotic activation of the intrinsic mitochondrial pathway". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4041-4046. https://doi.org/10.3892/etm.2017.5056
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