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Article

Preliminary screening of type IV secretion system in divergent geographic sources of Clostridium difficile

  • Authors:
    • Na Li
    • Hongbing Jia
    • Hui Yang
    • Bing Ji
    • Yongyun Liu
    • Xinguo Peng
    • Ying Cheng
    • Wen Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, The Affiliated Hospital of Binzhou Medical University, Binzhou, Shandong 256600, P.R. China, Department of Clinical Laboratory, China‑Japan Friendship Hospital, Beijing 100029, P.R. China, Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, P.R. China
  • Pages: 4405-4410
    |
    Published online on: August 29, 2017
       https://doi.org/10.3892/etm.2017.5065
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Abstract

In the present study, different geographical sources and sequence types (STs) of Clostridium difficile were preliminarily screened to investigate the distribution profiles of three core genes, VirB4, VirB6 and VirD4, of the type IV secretion system (T4SS). A total of 37 C. difficile strains from different sources were screened, inoculated and prepared for genome extraction. C. difficile toxins A and B were subjected to identification and multilocus sequence typing (MLST) analysis. The T4SS gene then underwent polymerase chain reaction amplification and sequencing analysis. Of the 37 strains, 25 were toxin A‑ and toxin B‑positive, and 12 were toxin A‑negative and toxin B‑positive. MLST detected 11 strains with ST37, 10 with ST2, 6 with ST35, 7 with ST3, 1 with ST54, 1 with ST1 and 1 with ST119. The detection rates of VirB4, VirB6 and VirD4 were all 100% in colonies exhibiting T4SS. Single nucleotide polymorphisms (SNPs) were detected in a minority of strains. C. difficile strains with identical STs shared the same SNP loci for T4SS, and those with different STs had different SNP loci. The results of the present study may provide evidence for subsequent identification of T4SS distribution, epidemiological investigations, polymorphism analyses and research into the association between T4SS, cytotoxicity and enterotoxication in C. difficile.
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1 

Bauer MP, Notermans DW, van Benthem BH, Brazier JS, Wilcox MH, Rupnik M, Monnet DL, van Dissel JT and Kuijper EJ: ECDIS Study Group: Clostridium difficile infection in Europe: A hospital-based survey. The Lancet. 377:63–73. 2011. View Article : Google Scholar

2 

Kuijper EJ, Coignard B and Tüll P: ESCMID Study Group for Clostridium difficile; EU Member States; European Centre for Disease Prevention and Control: Emergence of Clostridium difficile-associated disease in North America and Europe. Clin Microbiol Infect. 12 Suppl 6:S2–S18. 2006. View Article : Google Scholar

3 

Huang H, Wu S, Wang M, Zhang Y, Fang H, Palmgren AC, Weintraub A and Nord CE: Clostridium difficile infections in a Shanghai hospital: Antimicrobial resistance, toxin profiles and ribotypes. Int J Antimicrob Agents. 33:339–342. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Wilkins TD and Lyerly DM: Clostridium difficile testing: After 20 years, still challenging. J Clin Microbiol. 41:531–534. 2003. View Article : Google Scholar : PubMed/NCBI

5 

Zhang W, Rong C, Chen C and Gao GF: Type-IVC Secretion system: A novel subclass of type IV secretion system (T4SS) common existing in gram-positive genus Streptococcus. Plos One. 7:e463902012. View Article : Google Scholar : PubMed/NCBI

6 

Rupnik M: How to detect Clostridium difficile variant strains in a routine laboratory. Clin Microbiol Infect. 7:417–420. 2001. View Article : Google Scholar : PubMed/NCBI

7 

He M, Sebaihia M, Lawley TD, Stabler RA, Dawson LF, Martin MJ, Holt KE, Seth-Smith HM, Quail MA, Rance R, et al: Evolutionary dynamics of Clostridium difficile over short and long time scales. Proc Natl Acad Sci USA. 107:7527–7532. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Janvilisri T, Scaria J, Thompson AD, Nicholson A, Limbago BM, Arroyo LG, Songer JG, Gröhn YT and Chang YF: Microarray identification of Clostridium difficile core components and divergent regions associated with host origin. J Bacteriol. 191:3881–3891. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Yan Q, Zhang J, Chen C, Zhou H, Du P, Cui Z, Cen R, Liu L, Li W, Cao B, et al: Multilocus sequence typing (MLST) analysis of 104 Clostridium difficile strains from China. Epidemiol Infect. 4:195–199. 2013. View Article : Google Scholar

10 

Lemme L, Halluin A, Pestel-Caron M, Lemeland JF and Pons JL: Multilocus sequence typing analysis of human and animal Clostridium difficile isolates of various toxigenic types. J Clin Microbiol. 42:2609–2617. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Kato H, Kato N, Watanabe K, Iwai N, Nakamura H, Yamamoto T, Suzuki K, Kim SM, Chong Y and Wasito EB: Identification of toxin A-negative, toxin B-positive Clostridium difficile by PCR. J Clin Microbiol. 36:2178–2182. 1998.PubMed/NCBI

12 

Griffiths D, Fawley W, Kachrimanidou M, Bowden R, Crook DW, Fung R, Golubchik T, Harding RM, Jeffery KJ, Jolley KA, et al: Multilocus sequence typing of Clostridium difficile. J ClinMicrobiol. 48:770–778. 2010.

13 

Cheng Y, Du P, Chen C, Yan S, Jia H, Wang J, Yan Q, Feng H and Lu J: Toxin A-negative, toxin B-positive Clostridium difficile infection diagnosed by polymerase chain reaction. Infect Control Hosp Epidemiol. 32:520–522. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Govind R and Dupuy B: Secretion of Clostridium difficile toxins A and B requires the holin-like protein TcdE. PLoS Pathog. 8:e10027272012. View Article : Google Scholar : PubMed/NCBI

15 

Olling A, Seehase S, Minton NP, Tatge H, Schröter S, Kohlscheen S, Pich A, Just I and Gerhard R: Release of TcdA and TcdB from Clostridium difficile cdi 630 is not affected by functional inactivation of the tcdE gene. Microb Pathog. 52:92–100. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Brouwer MS, Roberts AP, Hussain H, Williams RJ, Allan E and Mullany P: Horizontal gene transfer converts non-toxigenic Clostridium difficile strains into toxin producers. Nat Commun. 4:20612013. View Article : Google Scholar : PubMed/NCBI

17 

Segal G, Feldman M and Zusman T: The Icm/Dot type-IV secretion systems of Legionella pneumophila and Coxiella burnetii. FEMS Microbiol Rev. 29:65–81. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Ninio S and Roy CR: Effector proteins translocated by Legionella pneumophila: Strength in numbers. Trends Microbiol. 15:372–380. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Wang P, Xiong Y, Lan R, Ye C, Wang H, Ren J, Jing H, Wang Y, Zhou Z, Cui Z, et al: pO157_Sal, a novel conjugative plasmid detected in outbreak isolates of escherichia coli O157:H7. J Clin Microbiol. 49:1594–1597. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Saenz HL, Engel P, Stoeckli MC, Lanz C, Raddatz G, Vayssier-Taussat M, Birtles R, Schuster SC and Dehio C: Genomic analysis of Bartonella identifies type IV secretion systems as host adaptability factors. Nat Genet. 39:1469–1476. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li N, Jia H, Yang H, Ji B, Liu Y, Peng X, Cheng Y and Zhang W: Preliminary screening of type IV secretion system in divergent geographic sources of Clostridium difficile. Exp Ther Med 14: 4405-4410, 2017.
APA
Li, N., Jia, H., Yang, H., Ji, B., Liu, Y., Peng, X. ... Zhang, W. (2017). Preliminary screening of type IV secretion system in divergent geographic sources of Clostridium difficile. Experimental and Therapeutic Medicine, 14, 4405-4410. https://doi.org/10.3892/etm.2017.5065
MLA
Li, N., Jia, H., Yang, H., Ji, B., Liu, Y., Peng, X., Cheng, Y., Zhang, W."Preliminary screening of type IV secretion system in divergent geographic sources of Clostridium difficile". Experimental and Therapeutic Medicine 14.5 (2017): 4405-4410.
Chicago
Li, N., Jia, H., Yang, H., Ji, B., Liu, Y., Peng, X., Cheng, Y., Zhang, W."Preliminary screening of type IV secretion system in divergent geographic sources of Clostridium difficile". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4405-4410. https://doi.org/10.3892/etm.2017.5065
Copy and paste a formatted citation
x
Spandidos Publications style
Li N, Jia H, Yang H, Ji B, Liu Y, Peng X, Cheng Y and Zhang W: Preliminary screening of type IV secretion system in divergent geographic sources of Clostridium difficile. Exp Ther Med 14: 4405-4410, 2017.
APA
Li, N., Jia, H., Yang, H., Ji, B., Liu, Y., Peng, X. ... Zhang, W. (2017). Preliminary screening of type IV secretion system in divergent geographic sources of Clostridium difficile. Experimental and Therapeutic Medicine, 14, 4405-4410. https://doi.org/10.3892/etm.2017.5065
MLA
Li, N., Jia, H., Yang, H., Ji, B., Liu, Y., Peng, X., Cheng, Y., Zhang, W."Preliminary screening of type IV secretion system in divergent geographic sources of Clostridium difficile". Experimental and Therapeutic Medicine 14.5 (2017): 4405-4410.
Chicago
Li, N., Jia, H., Yang, H., Ji, B., Liu, Y., Peng, X., Cheng, Y., Zhang, W."Preliminary screening of type IV secretion system in divergent geographic sources of Clostridium difficile". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4405-4410. https://doi.org/10.3892/etm.2017.5065
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