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Upregulation of microRNA‑206 induces apoptosis of vascular smooth muscle cells and decreases risk of atherosclerosis through modulating FOXP1

  • Authors:
    • Tao Xing
    • Lixin Du
    • Xianbo Zhuang
    • Liyong Zhang
    • Jiheng Hao
    • Jiyue Wang
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Liaocheng People's Hospital, Liaocheng, Shandong 252000, P.R. China, Department of Neurology, Liaocheng People's Hospital, Liaocheng, Shandong 252000, P.R. China, Department of Neurosurgery, Brain Hospital of Liaocheng, Liaocheng, Shandong 252000, P.R. China
    Copyright: © Xing et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4097-4103
    |
    Published online on: August 30, 2017
       https://doi.org/10.3892/etm.2017.5071
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Abstract

Forkhead box protein subfamily P (FOXP) 1 has an important role in the control of gene transcription and is also reported to function as a tumor suppressor. The aim of the present study was to explore the regulatory mechanisms of atherosclerosis by investigating the function of microRNA‑206 (miR‑206) and the regulatory association between miR‑206 and its potential target gene, FOXP1, in vascular smooth muscle cells (VSMCs). Bioinformatics tools were utilized to identify FOXP1 as a target of miR‑206. Luciferase reporter analysis was used to confirm this relationship and to identify the miR‑206 binding site in the FOXP1 3'‑untranslated region. It was demonstrated that the relative survival rate of VSMCs was suppressed by miR‑206 compared with scramble controls. Furthermore, reduced expression of miR‑206 in atherosclerosis tissue samples was observed, and the mRNA and protein expression levels of FOXP1 were upregulated in atherosclerosis tissue samples, both compared with the controls, indicating a negative correlation between miR‑206 and FOXP1. Additionally, when treated with miR‑206 mimics, the relative survival rate of VSMCs was notably reduced, which was rescued by overexpression of FOXP1. These findings increased the understanding of the regulatory role of miR‑206 in atherosclerosis in VSMCs via targeting the FOXP1 gene; therefore, intervention with miR‑206 as a therapeutic technique may be a strategy for atherosclerosis treatment in the future.
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1 

Go AS, Mozaffarian D, Roger VL, Benjamin EJ, Berry JD, Blaha MJ, Dai S, Ford ES, Fox CS, Franco S, et al: Heart disease and stroke statistics-2014 update: A report from the American Heart Association. Circulation. 129:e28–e292. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Poole-Wilson P: The prevention of cardiovascular disease worldwide: Whose task and WHO's task? Clin Med (Lond). 5:379–384. 2005. View Article : Google Scholar : PubMed/NCBI

3 

McLaren JE, Michael DR, Ashlin TG and Ramji DP: Cytokines, macrophage lipid metabolism and foam cells: Implications for cardiovascular disease therapy. Prog Lipid Res. 50:331–347. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Doran AC, Meller N and McNamara CA: Role of smooth muscle cells in the initiation and early progression of atherosclerosis. Arterioscler Thromb Vasc Biol. 28:812–819. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Lemmens K, Doggen K and De Keulenaer GW: Role of neuregulin-1/ErbB signaling in cardiovascular physiology and disease: Implications for therapy of heart failure. Circulation. 116:954–960. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Marx SO, Totary-Jain H and Marks AR: Vascular smooth muscle cell proliferation in restenosis. Circ Cardiovasc Interv. 4:104–111. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Rensen SS, Doevendans PA and van Eys GJ: Regulation and characteristics of vascular smooth muscle cell phenotypic diversity. Neth Heart J. 15:100–108. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Li L, Zhang HN, Chen HZ, Gao P, Zhu LH, Li HL, Lv X, Zhang QJ, Zhang R, Wang Z, et al: SIRT1 acts as a modulator of neointima formation following vascular injury in mice. Circ Res. 108:1180–1189. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Bartel DP: MicroRNAs: Target recognition and regulatory functions. Cell. 136:215–233. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Nana-Sinkam SP and Croce CM: MicroRNAs as therapeutic targets in cancer. Transl Res. 157:216–225. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Dai R and Ahmed SA: MicroRNA, a new paradigm for understanding immunoregulation, inflammation, and autoimmune diseases. Transl Res. 157:163–179. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Santovito D, Mezzetti A and Cipollone F: MicroRNAs and atherosclerosis: New actors for an old movie. Nutr Metab Cardiovasc Dis. 22:937–943. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Santovito D, De Nardis V, Marcantonio P, Mandolini C, Paganelli C, Vitale E, Buttitta F, Bucci M, Mezzetti A, Consoli A and Cipollone F: Plasma exosome microRNA profiling unravels a new potential modulator of adiponectin pathway in diabetes: Effect of glycemic control. J Clin Endocrinol Metab. 99:E1681–E1685. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Nazari-Jahantigh M, Wei Y and Schober A: The role of microRNAs in arterial remodelling. Thromb Haemost. 107:611–618. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Kang H and Hata A: MicroRNA regulation of smooth muscle gene expression and phenotype. Curr Opin Hematol. 19:224–231. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Ma X, Ma C and Zheng X: MicroRNA-155 in the pathogenesis of atherosclerosis: A conflicting role? Heart Lung Circ. 22:811–818. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Raitoharju E, Oksala N and Lehtimäki T: MicroRNAs in the atherosclerotic plaque. Clin Chem. 59:1708–1721. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Shu W, Yang H, Zhang L, Lu MM and Morrisey EE: Characterization of a new subfamily of winged-helix/forkhead (Fox) genes that are expressed in the lung and act as transcriptional repressors. J Biol Chem. 276:27488–27497. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Bot PT, Grundmann S, Goumans MJ, de Kleijn D, Moll F, de Boer O, van der Wal AC, van Soest A, de Vries JP, van Royen N, et al: Forkhead box protein P1 as a downstream target of transforming growth factor-β induces collagen synthesis and correlates with a more stable plaque phenotype. Atherosclerosis. 218:33–43. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Mandolini C, Santovito D, Marcantonio P, Buttitta F, Bucci M, Ucchino S, Mezzetti A and Cipollone F: Identification of microRNAs 758 and 33b as potential modulators of ABCA1 expression in human atherosclerotic plaques. Nutr Metab Cardiovasc Dis. 25:202–209. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Ernst O and Zor T: Linearization of the bradford protein assay. J Vis Exp. 12:pii: 1918. 2010.

22 

Abeyesinghe SM, Nicol CJ, Wathes CM and Randall JM: Development of a raceway method to assess aversion of domestic fowl to concurrent stressors. Behav Processes. 56:175–194. 2001. View Article : Google Scholar : PubMed/NCBI

23 

Dey BK, Gagan J and Dutta A: miR-206 and −486 induce myoblast differentiation by downregulating Pax7. Mol Cell Biol. 31:203–214. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Anderson C, Catoe H and Werner R: MIR-206 regulates connexin43 expression during skeletal muscle development. Nucleic Acids Res. 34:5863–5871. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Jalali S, Ramanathan GK, Parthasarathy PT, Aljubran S, Galam L, Yunus A, Garcia S, Cox RR Jr, Lockey RF and Kolliputi N: Mir-206 regulates pulmonary artery smooth muscle cell proliferation and differentiation. PLoS One. 7:e468082012. View Article : Google Scholar : PubMed/NCBI

26 

Song G, Zhang Y and Wang L: MicroRNA-206 targets notch3, activates apoptosis, and inhibits tumor cell migration and focus formation. J Biol Chem. 284:31921–31927. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Chen X, Yan Q, Li S, Zhou L, Yang H, Yang Y, Liu X and Wan X: Expression of the tumor suppressor miR-206 is associated with cellular proliferative inhibition and impairs invasion in ERα-positive endometrioid adenocarcinoma. Cancer Lett. 314:41–53. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Kondo N, Toyama T, Sugiura H, Fujii Y and Yamashita H: miR-206 Expression is down-regulated in estrogen receptor alpha-positive human breast cancer. Cancer Res. 68:5004–5008. 2008. View Article : Google Scholar : PubMed/NCBI

29 

Chatterjee A and Hage FG: Guidelines in review: 2014 ACC/AHA guideline on perioperative cardiovascular evaluation and management of patients undergoing noncardiac surgery: A report of the American College of Cardiology/American Heart Association Task Force on practice guidelines. J Nucl Cardiol. 22:158–161. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Banham AH, Beasley N, Campo E, Fernandez PL, Fidler C, Gatter K, Jones M, Mason DY, Prime JE, Trougouboff P, et al: The FOXP1 winged helix transcription factor is a novel candidate tumor suppressor gene on chromosome 3p. Cancer Res. 61:8820–8829. 2001.PubMed/NCBI

31 

Zhang Y, Li S, Yuan L, Tian Y, Weidenfeld J, Yang J, Liu F, Chokas AL and Morrisey EE: Foxp1 coordinates cardiomyocyte proliferation through both cell-autonomous and nonautonomous mechanisms. Genes Dev. 24:1746–1757. 2010. View Article : Google Scholar : PubMed/NCBI

32 

Caruso P, MacLean MR, Khanin R, McClure J, Soon E, Southgate M, MacDonald RA, Greig JA, Robertson KE, Masson R, et al: Dynamic changes in lung microRNA profiles during the development of pulmonary hypertension due to chronic hypoxia and monocrotaline. Arterioscler Thromb Vasc Biol. 30:716–723. 2010. View Article : Google Scholar : PubMed/NCBI

33 

Bonnet S and Archer SL: Potassium channel diversity in the pulmonary arteries and pulmonary veins: Implications for regulation of the pulmonary vasculature in health and during pulmonary hypertension. Pharmacol Ther. 115:56–69. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xing T, Du L, Zhuang X, Zhang L, Hao J and Wang J: Upregulation of microRNA‑206 induces apoptosis of vascular smooth muscle cells and decreases risk of atherosclerosis through modulating FOXP1. Exp Ther Med 14: 4097-4103, 2017.
APA
Xing, T., Du, L., Zhuang, X., Zhang, L., Hao, J., & Wang, J. (2017). Upregulation of microRNA‑206 induces apoptosis of vascular smooth muscle cells and decreases risk of atherosclerosis through modulating FOXP1. Experimental and Therapeutic Medicine, 14, 4097-4103. https://doi.org/10.3892/etm.2017.5071
MLA
Xing, T., Du, L., Zhuang, X., Zhang, L., Hao, J., Wang, J."Upregulation of microRNA‑206 induces apoptosis of vascular smooth muscle cells and decreases risk of atherosclerosis through modulating FOXP1". Experimental and Therapeutic Medicine 14.5 (2017): 4097-4103.
Chicago
Xing, T., Du, L., Zhuang, X., Zhang, L., Hao, J., Wang, J."Upregulation of microRNA‑206 induces apoptosis of vascular smooth muscle cells and decreases risk of atherosclerosis through modulating FOXP1". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4097-4103. https://doi.org/10.3892/etm.2017.5071
Copy and paste a formatted citation
x
Spandidos Publications style
Xing T, Du L, Zhuang X, Zhang L, Hao J and Wang J: Upregulation of microRNA‑206 induces apoptosis of vascular smooth muscle cells and decreases risk of atherosclerosis through modulating FOXP1. Exp Ther Med 14: 4097-4103, 2017.
APA
Xing, T., Du, L., Zhuang, X., Zhang, L., Hao, J., & Wang, J. (2017). Upregulation of microRNA‑206 induces apoptosis of vascular smooth muscle cells and decreases risk of atherosclerosis through modulating FOXP1. Experimental and Therapeutic Medicine, 14, 4097-4103. https://doi.org/10.3892/etm.2017.5071
MLA
Xing, T., Du, L., Zhuang, X., Zhang, L., Hao, J., Wang, J."Upregulation of microRNA‑206 induces apoptosis of vascular smooth muscle cells and decreases risk of atherosclerosis through modulating FOXP1". Experimental and Therapeutic Medicine 14.5 (2017): 4097-4103.
Chicago
Xing, T., Du, L., Zhuang, X., Zhang, L., Hao, J., Wang, J."Upregulation of microRNA‑206 induces apoptosis of vascular smooth muscle cells and decreases risk of atherosclerosis through modulating FOXP1". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4097-4103. https://doi.org/10.3892/etm.2017.5071
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