Upregulation of microRNA‑206 induces apoptosis of vascular smooth muscle cells and decreases risk of atherosclerosis through modulating FOXP1

Xing,Tao; Du,Lixin; Zhuang,Xianbo; Zhang,Liyong; Hao,Jiheng; Wang,Jiyue

doi:10.3892/etm.2017.5071

Upregulation of microRNA‑206 induces apoptosis of vascular smooth muscle cells and decreases risk of atherosclerosis through modulating FOXP1

Authors:
- Tao Xing
- Lixin Du
- Xianbo Zhuang
- Liyong Zhang
- Jiheng Hao
- Jiyue Wang
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Affiliations: Department of Neurosurgery, Liaocheng People's Hospital, Liaocheng, Shandong 252000, P.R. China, Department of Neurology, Liaocheng People's Hospital, Liaocheng, Shandong 252000, P.R. China, Department of Neurosurgery, Brain Hospital of Liaocheng, Liaocheng, Shandong 252000, P.R. China
Published online on: August 30, 2017 https://doi.org/10.3892/etm.2017.5071
Pages: 4097-4103
Copyright: © Xing et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Forkhead box protein subfamily P (FOXP) 1 has an important role in the control of gene transcription and is also reported to function as a tumor suppressor. The aim of the present study was to explore the regulatory mechanisms of atherosclerosis by investigating the function of microRNA‑206 (miR‑206) and the regulatory association between miR‑206 and its potential target gene, FOXP1, in vascular smooth muscle cells (VSMCs). Bioinformatics tools were utilized to identify FOXP1 as a target of miR‑206. Luciferase reporter analysis was used to confirm this relationship and to identify the miR‑206 binding site in the FOXP1 3'‑untranslated region. It was demonstrated that the relative survival rate of VSMCs was suppressed by miR‑206 compared with scramble controls. Furthermore, reduced expression of miR‑206 in atherosclerosis tissue samples was observed, and the mRNA and protein expression levels of FOXP1 were upregulated in atherosclerosis tissue samples, both compared with the controls, indicating a negative correlation between miR‑206 and FOXP1. Additionally, when treated with miR‑206 mimics, the relative survival rate of VSMCs was notably reduced, which was rescued by overexpression of FOXP1. These findings increased the understanding of the regulatory role of miR‑206 in atherosclerosis in VSMCs via targeting the FOXP1 gene; therefore, intervention with miR‑206 as a therapeutic technique may be a strategy for atherosclerosis treatment in the future.

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