Evaluation of the diagnostic performance of panfungal polymerase chain reaction assay in invasive fungal diseases

Cao,Guo‑Jun; Xing,Zhi‑Fang; Hua,Li; Ji,Yu‑Hua; Sun,Jia‑Bin; Zhao,Zhen

doi:10.3892/etm.2017.5081

Evaluation of the diagnostic performance of panfungal polymerase chain reaction assay in invasive fungal diseases

Authors:
- Guo‑Jun Cao
- Zhi‑Fang Xing
- Li Hua
- Yu‑Hua Ji
- Jia‑Bin Sun
- Zhen Zhao
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Affiliations: Department of Laboratory Medicine, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China, Department of Blood Transfusion, Minhang Hospital, Fudan University, Shanghai 201199, P.R. China, Department of Clinical Laboratory, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China, Department of Clinical Laboratory, Minhang Hospital, Fudan University, Shanghai 201199, P.R. China
Published online on: August 31, 2017 https://doi.org/10.3892/etm.2017.5081
Pages: 4208-4214
Copyright: © Cao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Timely diagnosis of invasive fungal diseases (IFDs) is important, as delays in treatment initiation are associated with increased mortality rates. However, early diagnosis of IFDs in immunocompromised patients remains difficult. The conventional diagnostic methods currently used for IFDs are not sufficiently effective. Molecular tests, such as polymerase chain reaction (PCR)‑based assays, have great potential to improve the early diagnosis of IFDs due to their sensitivity and specificity. In the present study, the diagnostic performance of panfungal PCR assays in IFD patients who received bone marrow transplantation was evaluated. The results suggested that panfungal PCR assay offered a quick and convenient guide for clinical decision‑making by identifying higher numbers of fungal species in comparison with the conventional blood culture method. Furthermore, panfungal PCR assay exhibited a sensitivity of 93% and a specificity of 71% in the diagnosis of IFD patients based on the EORTC/MSG criteria. Thus, the present study concluded that the reported PCR‑based method was effective and sensitive in early IFD diagnosis and should be integrated into clinical decision‑making for the treatment of IFDs in the future.

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