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Article

Synergistic enhancement of matrix metalloproteinase‑9 expression and pro‑inflammatory cytokines by influenza virus infection and oxidized‑LDL treatment in human endothelial cells

  • Authors:
    • Yun Wu
    • He Huang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiovascular Medicine, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
  • Pages: 4579-4585
    |
    Published online on: September 1, 2017
       https://doi.org/10.3892/etm.2017.5099
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Abstract

Oxidized low‑density lipoprotein (oxLDL) has been reported to contribute to the development and progression of atherosclerosis, which is also stimulated by viral infections, such as influenza. However, the mechanism underlining the promotion of atherosclerosis by both risk factors remains unclear. In the present study, we investigated the expression of matrix metalloproteinase‑9 (MMP‑9), which is one of key mediators of atherosclerosis progression, in oxLDL‑treated human umbilical vein endothelial cells (HUVEC)‑C cells. The infection efficiency of H1N1 pdm2009 influenza virus in the HUVEC‑C cells was subsequently examined, and the expression of MMP‑9 and proinflammatory cytokines, including tumor necrosis factor (TNF)‑α, interleukin (IL)‑1β and IL‑6, were determined in the virus‑infected HUVEC‑C cells, with or without oxLDL treatment. Results demonstrated that oxLDL treatment with 10, 20 or 50 µg/ml markedly upregulated MMP‑9 expression at the mRNA and protein levels. H1N1 pdm2009 influenza virus efficiently infected the HUVEC‑C cells and significantly promoted the expression of MMP‑9, TNF‑α, IL‑1β and IL‑6, synergistically with the oxLDL treatment. Taken together, these results demonstrated for the first time that oxidized‑LDL treatment and influenza virus infection synergistically enhance the expression of MMP‑9 and proinflammatory cytokines in human endothelial cells, suggesting that both factors are potent stimulators in atherosclerotic impairment to endothelial cells.
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1 

Ehara S, Ueda M, Naruko T, Haze K, Itoh A, Otsuka M, Komatsu R, Matsuo T, Itabe H and Takano T: Elevated levels of oxidized low density lipoprotein show a positive relationship with the severity of acute coronary syndromes. Circulation. 103:1955–1960. 2001. View Article : Google Scholar : PubMed/NCBI

2 

Verhoye E and Langlois: Asklepios Investigators: Circulating oxidized low-density lipoprotein: A biomarker of atherosclerosis and cardiovascular risk? Clin Chem Lab Med. 47:128–137. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Itabe H: Oxidized low-density lipoprotein as a biomarker of in vivo oxidative stress: From atherosclerosis to periodontitis. J Clin Biochem Nutr. 51:1–8. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Imanishi T, Hano T, Sawamura T, Takarada S and Nishio I: Oxidized low density lipoprotein potentiation of Fas-induced apoptosis through lectin-like oxidized-low density lipoprotein receptor-1 in human umbilical vascular endothelial cells. Circ J. 66:1060–1064. 2002. View Article : Google Scholar : PubMed/NCBI

5 

Bekkering S, Quintin J, Joosten LA, van der Meer JW, Netea MG and Riksen NP: Oxidized low-density lipoprotein induces long-term proinflammatory cytokine production and foam cell formation via epigenetic reprogramming of monocytes. Arterioscler Thromb Vasc Biol. 34:1731–1738. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Chen J, Mehta JL, Haider N, Zhang X, Narula J and Li D: Role of caspases in Ox-LDL-induced apoptotic cascade in human coronary artery endothelial cells. Circ Res. 94:370–376. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Galis ZS, Sukhova GK, Lark MW and Libby P: Increased expression of matrix metalloproteinases and matrix degrading activity in vulnerable regions of human atherosclerotic plaques. J Clin Invest. 94:2493–2503. 1994. View Article : Google Scholar : PubMed/NCBI

8 

Falk E, Shah PK and Fuster V: Coronary plaque disruption. Circulation. 92:657–671. 1995. View Article : Google Scholar : PubMed/NCBI

9 

Galis ZS and Khatri JJ: Matrix metalloproteinases in vascular remodeling and atherogenesis: The good, the bad and the ugly. Circ Res. 90:251–262. 2002.PubMed/NCBI

10 

Visse R and Nagase H: Matrix metalloproteinases and tissue inhibitors of metalloproteinases: Structure, function and biochemistry. Circ Res. 92:827–839. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Kuzuya M and Iguchi A: Role of matrix metalloproteinases in vascular remodeling. J Atheroscler Thromb. 10:275–282. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Flamant M, Placier S, Dubroca C, Esposito B, Lopes I, Chatziantoniou C, Tedgui A, Dussaule JC and Lehoux S: Role of matrix metalloproteinases in early hypertensive vascular remodeling. Hypertension. 50:212–218. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Sukhova GK, Schonbeck U, Rabkin E, Schoen FJ, Poole AR, Billinghurst RC and Libby P: Evidence for increased collagenolysis by interstitial collagenases-1 and −3 in vulnerable human atheromatous plaques. Circulation. 99:2503–2509. 1999. View Article : Google Scholar : PubMed/NCBI

14 

Herman MP, Sukhova GK, Libby P, Gerdes N, Tang N, Horton DB, Kilbride M, Breitbart RE, Chun M and Schönbeck U: Expression of neutrophil collagenase (matrix metalloproteinase-8) in human atheroma: A novel collagenolytic pathway suggested by transcriptional profiling. Circulation. 104:1899–1904. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Nikkari ST, O'Brien KD, Ferguson M, Hatsukami T, Welgus HG, Alpers CE and Clowes AW: Interstitial collagenase (MMP-1) expression in human carotid atherosclerosis. Circulation. 92:1393–1398. 1995. View Article : Google Scholar : PubMed/NCBI

16 

Rajavashisth TB, Xu XP, Jovinge S, Meisel S, Xu XO, Chai NN, Fishbein MC, Kaul S, Cercek B, Sharifi B and Shah PK: Membrane type 1 matrix metalloproteinase expression in human atherosclerotic plaques: Evidence for activation by proinflammatory mediators. Circulation. 99:3103–3109. 1999. View Article : Google Scholar : PubMed/NCBI

17 

Xu XP, Meisel SR, Ong JM, Kaul S, Cercek B, Rajavashisth TB, Sharifi B and Shah PK: Oxidized low-density lipoprotein regulates matrix metalloproteinase-9 and its tissue inhibitor in human monocyte-derived macrophages. Circulation. 99:993–998. 1999. View Article : Google Scholar : PubMed/NCBI

18 

Roivainen M, Viik-Kajander M, Palosuo T, Toivanen P, Leinonen M, Saikku P, Tenkanen L, Manninen V, Hovi T and Mänttäri M: Infections, inflammation and the risk of coronary heart disease. Circulation. 101:252–257. 2000. View Article : Google Scholar : PubMed/NCBI

19 

Ravnskov U and McCully KS: Infections may be causal in the pathogenesis of atherosclerosis. Am J Med Sci. 344:391–394. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Hechter RC, Budoff M, Hodis HN, Rinaldo CR, Jenkins FJ, Jacobson LP, Kingsley LA, Taiwo B, Post WS, Margolick JB and Detels R: Herpes simplex virus type 2 (HSV-2) as a coronary atherosclerosis risk factor in HIV-infected men: Multicenter AIDS cohort study. Atherosclerosis. 223:433–436. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Gurevich VS, Pleskov VM, Levaia MV, Bannikov AI, Mitrofanova LB and Urazgil'Deeva SA: Influenza virus infection in progressing atherosclerosis. Kardiologiia. 42:21–24. 2002.PubMed/NCBI

22 

Auer J, Berent R, Weber T and Eber B: Influenza virus infection, infectious burden and atherosclerosis. Stroke. 33:1454–1455. 2002. View Article : Google Scholar : PubMed/NCBI

23 

Streblow DN, Orloff SL and Nelson JA: Do pathogens accelerate atherosclerosis? J Nutr. 131:2798S–2804S. 2001.PubMed/NCBI

24 

Söderberg-Nauclér C: Does cytomegalovirus play a causative role in the development of various inflammatory diseases and cancer? J Intern Med. 259:219–246. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Epstein SE, Zhou YF and Zhu J: Infection and atherosclerosis: Emerging mechanistic paradigms. Circulation. 100:e20–e28. 1999. View Article : Google Scholar : PubMed/NCBI

26 

Libby P, Ridker PM and Maseri A: Inflammation and atherosclerosis. Circulation. 105:1135–1143. 2002. View Article : Google Scholar : PubMed/NCBI

27 

Nieto FJ, Adam E, Sorlie P, Farzadegan H, Melnick JL, Comstock GW and Szklo M: Cohort study of cytomegalovirus infection as a risk factor for carotid intimal-medial thickening, a measure of subclinical atherosclerosis. Circulation. 94:922–927. 1996. View Article : Google Scholar : PubMed/NCBI

28 

Smieja M, Gnarpe J, Lonn E, Gnarpe H, Olsson G, Yi Q, Dzavik V, McQueen M and Yusuf S: Heart Outcomes Prevention Evaluation (HOPE) Study Investigators: Multiple infections and subsequent cardiovascular events in the heart outcomes prevention evaluation (HOPE) study. Circulation. 107:251–257. 2003. View Article : Google Scholar : PubMed/NCBI

29 

Celik S, Shankar V, Richter A, Hippe HJ, Akhavanpoor M, Bea F, Erbel C, Urban S, Blank N, Wambsganss N and Katus HA: Proinflammatory and prothrombotic effects on human vascular endothelial cells of immune-cell-derived light. Eur J Med Res. 14:147–156. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

31 

Halade GV, Jin YF and Lindsey ML: Matrix metalloproteinase (MMP)-9: A proximal biomarker for cardiac remodeling and a distal biomarker for inflammation. Pharmacol Ther. 139:32–40. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Gao X, Xu X, Belmadani S, Park Y, Tang Z, Feldman AM, Chilian WM and Zhang C: TNF-alpha contributes to endothelial dysfunction by upregulating arginase in ischemia/reperfusion injury. Arterioscler Thromb Vasc Biol. 27:1269–1275. 2007. View Article : Google Scholar : PubMed/NCBI

33 

Haidari M, Wyde PR, Litovsky S, Vela D, Ali M, Casscells SW and Madjid M: Influenza virus directly infects, inflames and resides in the arteries of atherosclerotic and normal mice. Atherosclerosis. 208:90–96. 2010. View Article : Google Scholar : PubMed/NCBI

34 

Gurevich VS: Influenza, autoimmunity and atherogenesis. Autoimmun Rev. 4:101–105. 2005. View Article : Google Scholar : PubMed/NCBI

35 

Suo J, Zhao L, Wang J, Zhu Z, Zhang H and Gao R: Influenza virus aggravates the ox-LDL-induced apoptosis of human endothelial cells via promoting p53 signaling. J Med Virol. 87:1113–1123. 2015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wu Y and Huang H: Synergistic enhancement of matrix metalloproteinase‑9 expression and pro‑inflammatory cytokines by influenza virus infection and oxidized‑LDL treatment in human endothelial cells. Exp Ther Med 14: 4579-4585, 2017.
APA
Wu, Y., & Huang, H. (2017). Synergistic enhancement of matrix metalloproteinase‑9 expression and pro‑inflammatory cytokines by influenza virus infection and oxidized‑LDL treatment in human endothelial cells. Experimental and Therapeutic Medicine, 14, 4579-4585. https://doi.org/10.3892/etm.2017.5099
MLA
Wu, Y., Huang, H."Synergistic enhancement of matrix metalloproteinase‑9 expression and pro‑inflammatory cytokines by influenza virus infection and oxidized‑LDL treatment in human endothelial cells". Experimental and Therapeutic Medicine 14.5 (2017): 4579-4585.
Chicago
Wu, Y., Huang, H."Synergistic enhancement of matrix metalloproteinase‑9 expression and pro‑inflammatory cytokines by influenza virus infection and oxidized‑LDL treatment in human endothelial cells". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4579-4585. https://doi.org/10.3892/etm.2017.5099
Copy and paste a formatted citation
x
Spandidos Publications style
Wu Y and Huang H: Synergistic enhancement of matrix metalloproteinase‑9 expression and pro‑inflammatory cytokines by influenza virus infection and oxidized‑LDL treatment in human endothelial cells. Exp Ther Med 14: 4579-4585, 2017.
APA
Wu, Y., & Huang, H. (2017). Synergistic enhancement of matrix metalloproteinase‑9 expression and pro‑inflammatory cytokines by influenza virus infection and oxidized‑LDL treatment in human endothelial cells. Experimental and Therapeutic Medicine, 14, 4579-4585. https://doi.org/10.3892/etm.2017.5099
MLA
Wu, Y., Huang, H."Synergistic enhancement of matrix metalloproteinase‑9 expression and pro‑inflammatory cytokines by influenza virus infection and oxidized‑LDL treatment in human endothelial cells". Experimental and Therapeutic Medicine 14.5 (2017): 4579-4585.
Chicago
Wu, Y., Huang, H."Synergistic enhancement of matrix metalloproteinase‑9 expression and pro‑inflammatory cytokines by influenza virus infection and oxidized‑LDL treatment in human endothelial cells". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4579-4585. https://doi.org/10.3892/etm.2017.5099
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