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Combined effect of linolenic acid and tobramycin on Pseudomonas aeruginosa biofilm formation and quorum sensing

  • Authors:
    • Warren Chanda
    • Thomson Patrick Joseph
    • Arshad Ahmed Padhiar
    • Xuefang Guo
    • Liu Min
    • Wendong Wang
    • Sainyugu Lolokote
    • Anhong Ning
    • Jing Cao
    • Min Huang
    • Mintao Zhong
  • View Affiliations / Copyright

    Affiliations: Department of Microbiology, College of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning 116044 P.R. China, Department of Epidemiology and Biostatistics, School of Public Health, Dalian Medical University, Dalian, Liaoning 116044 P.R. China, Laboratory of Pathogen Biology, Experimental Teaching Center for Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning 116044 P.R. China
    Copyright: © Chanda et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4328-4338
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    Published online on: September 5, 2017
       https://doi.org/10.3892/etm.2017.5110
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Abstract

Pseudomonas aeruginosa is a ubiquitous Gram negative opportunistic pathogen capable of causing severe nosocomial infections in humans, and tobramycin is currently used to treat P. aeruginosa associated lung infections. Quorum sensing regulates biofilm formation which allows the bacterium to result in fatal infections forcing clinicians to extensively use antibiotics to manage its infections leading to emerging multiple drug resistant strains. As a result, tobramycin is also becoming resistant. Despite extensive studies on drug discovery to alleviate microbial drug resistance, the continued microbial evolution has forced researchers to focus on screening various phytochemicals and dietary compounds for antimicrobial potential. Linolenic acid (LNA) is an essential fatty acid that possesses antimicrobial actions on various microorganisms. It was hypothesized that LNA may affect the formation of biofilm on P. aeruginosa and improve the potency of tobramycin. The present study demonstrated that LNA interfered with cell‑to‑cell communication and reduced virulence factor production. It further enhanced the potency of tobramycin and synergistically inhibited biofilm formation through P. aeruginosa quorum sensing systems. Therefore, LNA may be considered as a potential agent for adjunctive therapy and its utilization may decrease tobramycin concentration in combined treatment thereby reducing aminoglycoside adverse effects.
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Chanda W, Joseph TP, Padhiar AA, Guo X, Min L, Wang W, Lolokote S, Ning A, Cao J, Huang M, Huang M, et al: Combined effect of linolenic acid and tobramycin on Pseudomonas aeruginosa biofilm formation and quorum sensing. Exp Ther Med 14: 4328-4338, 2017.
APA
Chanda, W., Joseph, T.P., Padhiar, A.A., Guo, X., Min, L., Wang, W. ... Zhong, M. (2017). Combined effect of linolenic acid and tobramycin on Pseudomonas aeruginosa biofilm formation and quorum sensing. Experimental and Therapeutic Medicine, 14, 4328-4338. https://doi.org/10.3892/etm.2017.5110
MLA
Chanda, W., Joseph, T. P., Padhiar, A. A., Guo, X., Min, L., Wang, W., Lolokote, S., Ning, A., Cao, J., Huang, M., Zhong, M."Combined effect of linolenic acid and tobramycin on Pseudomonas aeruginosa biofilm formation and quorum sensing". Experimental and Therapeutic Medicine 14.5 (2017): 4328-4338.
Chicago
Chanda, W., Joseph, T. P., Padhiar, A. A., Guo, X., Min, L., Wang, W., Lolokote, S., Ning, A., Cao, J., Huang, M., Zhong, M."Combined effect of linolenic acid and tobramycin on Pseudomonas aeruginosa biofilm formation and quorum sensing". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4328-4338. https://doi.org/10.3892/etm.2017.5110
Copy and paste a formatted citation
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Spandidos Publications style
Chanda W, Joseph TP, Padhiar AA, Guo X, Min L, Wang W, Lolokote S, Ning A, Cao J, Huang M, Huang M, et al: Combined effect of linolenic acid and tobramycin on Pseudomonas aeruginosa biofilm formation and quorum sensing. Exp Ther Med 14: 4328-4338, 2017.
APA
Chanda, W., Joseph, T.P., Padhiar, A.A., Guo, X., Min, L., Wang, W. ... Zhong, M. (2017). Combined effect of linolenic acid and tobramycin on Pseudomonas aeruginosa biofilm formation and quorum sensing. Experimental and Therapeutic Medicine, 14, 4328-4338. https://doi.org/10.3892/etm.2017.5110
MLA
Chanda, W., Joseph, T. P., Padhiar, A. A., Guo, X., Min, L., Wang, W., Lolokote, S., Ning, A., Cao, J., Huang, M., Zhong, M."Combined effect of linolenic acid and tobramycin on Pseudomonas aeruginosa biofilm formation and quorum sensing". Experimental and Therapeutic Medicine 14.5 (2017): 4328-4338.
Chicago
Chanda, W., Joseph, T. P., Padhiar, A. A., Guo, X., Min, L., Wang, W., Lolokote, S., Ning, A., Cao, J., Huang, M., Zhong, M."Combined effect of linolenic acid and tobramycin on Pseudomonas aeruginosa biofilm formation and quorum sensing". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4328-4338. https://doi.org/10.3892/etm.2017.5110
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