The expression and significance of tyrosine hydroxylase in the brain tissue of Parkinson's disease rats

Chen,Yuan; Lian,Yajun; Ma,Yunqing; Wu,Chuanjie; Zheng,Yake; Xie,Nanchang

doi:10.3892/etm.2017.5124

November-2017 Volume 14 Issue 5

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November-2017 Volume 14 Issue 5

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The expression and significance of tyrosine hydroxylase in the brain tissue of Parkinson's disease rats

Authors:
- Yuan Chen
- Yajun Lian
- Yunqing Ma
- Chuanjie Wu
- Yake Zheng
- Nanchang Xie
View Affiliations / Copyright

Affiliations: Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China

Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 4813-4816
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Published online on: September 18, 2017

https://doi.org/10.3892/etm.2017.5124
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Abstract

The expression and significance of tyrosine hydroxylase (TH) in brain tissue of rats with Parkinson's disease (PD) were explored and analyzed. A total of 120 clean‑grade and healthy adult Wistar rats weighing 180-240 g were randomly divided equally into four groups according to the random number table method. Rats were sacrificed before and after the model establishment for 3, 6 or 8 weeks. The number of revolutions in rats was observed and the relative expression of TH mRNA in brain tissue was measured. The relative expression of TH mRNA in left and right brain tissue of normal rats was respectively 1.039±0.112 and 0.956±0.120. There was no significant difference in the expression of TH mRNA in either side of the brain tissue (p>0.05). With the extension of modeling time, the success rate of model establishment was significantly improved (p<0.05). After the model establishment for 6 weeks, the success rate peaked and there was no significant difference with 8 weeks (p>0.05). The relative expression of TH mRNA in the right brain was 0.053±0.082, which was significantly lower than that in the left brain tissue with 1.056±0.094 (p<0.05). After 6 weeks, the relative expression of TH mRNA in the left and right brain of PD rats was respectively 1.004±0.034 and 0.316±0.012, the expression of TH mRNA in right brain tissue was also significantly lower than that in the left (p<0.05). Similarly, after 8 weeks of the model establishment, the relative expression of TH mRNA in the right brain was significantly lower than that in the left brain tissue, with 0.395±0.041 and 1.021±0.057 (p<0.05). Compared with the control group, the expression of TH mRNA in both sides of the brain tissue decreased, and the expression of TH mRNA in the injured brain tissue was significantly lower than that in normal rats (p<0.05). The expression of TH mRNA in brain tissue of PD rats was lower than that in normal brain tissue, which may be related to the occurrence and development of PD.

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1	Yang QQ, Sun FL, Al HX, Zhang L and Wang W: Assessment of 6-hydroxydopamine-lesion induced behavioral alteration as a rat model of Parkinson's disease. Acta Neuropharm. 1:17–22. 2013.
2	Li J, Wang LN and Xiao HL: Effect of electroacupuncture on oxidative stress injury of dopaminergic neurons in rats with Parkinson's disease. Acupunct Res. 39:185–191. 2014.
3	Leem J: Acupuncture for motor symptom improvement in Parkinson's disease and the potential identification of respondersto acupuncture treatment. Integr Med Res. 5:332–335. 2006. View Article : Google Scholar
4	Shavali S, Combs CK and Ebadi M: Reactive macrophages increase oxidative stress and alpha-synuclein nitration during death of dopaminergic neuronal cells in co-culture: Relevance to Parkinson's disease. Neurochem Res. 31:85–94. 2006. View Article : Google Scholar : PubMed/NCBI
5	Feve AP: Current status of tyrosine hydroxylase in management of Parkinson's disease. CNS Neurol Disord Drug Targets. 11:450–455. 2012. View Article : Google Scholar : PubMed/NCBI
6	Meng XJ, Pang Q, Ding F, Xin T and Yang HA: Establishment of PD rat model by injecting Taclo sterically and directionally in to striatum. J ShandongUniv. 52:16–18. 2014.(In Chinese).
7	Kashkin VA, Shekunova EV, Makarova MN and Makarov VG: A study of combination treatment with nacom (levodopa + carbodope) and citicoline in the model of Parkinson disease inrats. Zh Nevrol Psikhiatr Im S S Korsakova. 117:59–63. 2017.(In Russian). View Article : Google Scholar : PubMed/NCBI
8	Roche M, Frison G, Brion JD, Provot O, Hamze A and Alami M: Csp²-N bond formation via ligand-free Pd-catalyzed oxidative coupling reaction of N-tosylhydrazones and indole derivatives. J Org Chem. 78:8485–8495. 2013. View Article : Google Scholar : PubMed/NCBI
9	Nguyen Anh TN, Benatmane N, Fallahi V, Fang Y, Mohseni SM, Dumas RK and Åkerman J: [Co/Pd]₄-Co-Pd-NiFe spring magnets with highly tunable and uniform magnetization tilt angles. J Magn Magn Mater. 324:3929–3932. 2012. View Article : Google Scholar
10	McCabe K, Concannon RM, McKernan DP and Dowd E: Time-course of striatal Toll-like receptor expression inneurotoxic, environmental and inflammatory rat models of Parkinson's disease. J Neuroimmunol. 310:103–106. 2017. View Article : Google Scholar : PubMed/NCBI
11	Zheng LF, Zhang Y, Chen CL, Song J, Fan RF, Cai QQ, Wang ZY and Zhu JX: Alterations in TH- and ChAT-immunoreactive neurons in the DMV and gastric dysmotility in an LPS-induced PD rat model. Auton Neurosci. 177:194–198. 2013. View Article : Google Scholar : PubMed/NCBI
12	Michell AW, Tofaris GK, Gossage H, Tyers P, Spillantini MG and Barker RA: The effect of truncated human α-synuclein (1–120) on dopaminergic cells in a transgenic mouse model of Parkinson's Disease. Cell Transplant. 16:461–474. 2007. View Article : Google Scholar : PubMed/NCBI
13	Huang Y, Chang C, Zhang JW and Gao XQ: Alternation of proteins in brain of Parkinson's disease model rats after the transplantation of TH-NTN gene modified bone marrow mesenchymal stem cells. Zhonghua Yi Xue Za Zhi. 92:2353–2356. 2012.(In Chinese). PubMed/NCBI
14	Ali F, Taresh S, Al-Nuzaily M, Mok PL, Ismail A and Ahmad S: Stem cells differentiation and probing their therapeutic applications in hematological disorders: A critical review. Eur Rev Med Pharmacol Sci. 20:4390–4400. 2016.PubMed/NCBI
15	Thenral ST and Vanisree AJ: Peripheral assessment of the genes AQP4, PBP and TH in patients with Parkinson's disease. Neurochem Res. 37:512–515. 2012. View Article : Google Scholar : PubMed/NCBI
16	Zhao J: Effect of 3-n-butylphthalide on the expression of Bax caspase-8 and TH in the substantia nigra of PD rats. Chinese J Clin. 15:1288–1292. 2014.(In Chinese).

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

The expression and significance of tyrosine hydroxylase in the brain tissue of Parkinson's disease rats

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