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Extended magnetic resonance imaging studies on the effect of classically activated microglia transplantation on white matter regeneration following spinal cord focal injury in adult rats

  • Authors:
    • Wiesław Marcol
    • Wojciech Ślusarczyk
    • Magdalena Larysz‑Brysz
    • Krzysztof Łabuzek
    • Bartosz Kapustka
    • Rafał Staszkiewicz
    • Paulina Rosicka
    • Katarzyna Kalita
    • Władysław Węglarz
    • Joanna Lewin‑Kowalik
  • View Affiliations / Copyright

    Affiliations: Department of Physiology, School of Medicine in Katowice, Medical University of Silesia, 40‑752 Katowice, Poland, Department of Internal Medicine and Clinical Pharmacology, School of Medicine in Katowice, Medical University of Silesia, 40‑752 Katowice, Poland, Department of Magnetic Resonance Imaging, Institute of Nuclear Physics Polish Academy of Sciences, 31‑342 Kraków, Poland
    Copyright: © Marcol et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4869-4877
    |
    Published online on: September 19, 2017
       https://doi.org/10.3892/etm.2017.5130
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Abstract

Spinal cord injuries are still a serious problem for regenerative medicine. Previous research has demonstrated that activated microglia accumulate in spinal lesions, influencing the injured tissues in various ways. Therefore, transplantation of activated microglia may have a beneficial role in the regeneration of the nervous system. The present study examined the influence of transplanted activated microglial cells in adult rats with injured spinal cords. Rats were randomly divided into an experimental (M) and control (C) group, and were subjected to non‑laminectomy focal injury of spinal cord white matter by means of a high‑pressured air stream. In group M, activated cultured microglial cells were injected twice into the site of injury. Functional outcome and morphological features of regeneration were analyzed during a 12‑week follow‑up. The lesions were characterized by means of magnetic resonance imaging (MRI). Neurons in the brain stem and motor cortex were labeled with FluoroGold (FG). A total of 12 weeks after surgery, spinal cords and brains were collected and subjected to histopathological and immunohistochemical examinations. Lesion sizes in the spinal cord were measured and the number of FG‑positive neurons was counted. Rats in group M demonstrated significant improvement of locomotor performance when compared with group C (P<0.05). MRI analysis demonstrated moderate improvement in water diffusion along the spinal cord in the group M following microglia treatment, as compared with group C. The water diffusion perpendicular to the spinal cord in group M was closer to the reference values for a healthy spinal cord than it was in group C. The sizes of lesions were also significantly smaller in group M than in the group C (P<0.05). The number of brain stem and motor cortex FG‑positive neurons in group M was significantly higher than in group C. The present study demonstrated that delivery of activated microglia directly into the injured spinal cord gives some positive effects for the regeneration of the white matter.
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Copy and paste a formatted citation
Spandidos Publications style
Marcol W, Ślusarczyk W, Larysz‑Brysz M, Łabuzek K, Kapustka B, Staszkiewicz R, Rosicka P, Kalita K, Węglarz W, Lewin‑Kowalik J, Lewin‑Kowalik J, et al: Extended magnetic resonance imaging studies on the effect of classically activated microglia transplantation on white matter regeneration following spinal cord focal injury in adult rats. Exp Ther Med 14: 4869-4877, 2017.
APA
Marcol, W., Ślusarczyk, W., Larysz‑Brysz, M., Łabuzek, K., Kapustka, B., Staszkiewicz, R. ... Lewin‑Kowalik, J. (2017). Extended magnetic resonance imaging studies on the effect of classically activated microglia transplantation on white matter regeneration following spinal cord focal injury in adult rats. Experimental and Therapeutic Medicine, 14, 4869-4877. https://doi.org/10.3892/etm.2017.5130
MLA
Marcol, W., Ślusarczyk, W., Larysz‑Brysz, M., Łabuzek, K., Kapustka, B., Staszkiewicz, R., Rosicka, P., Kalita, K., Węglarz, W., Lewin‑Kowalik, J."Extended magnetic resonance imaging studies on the effect of classically activated microglia transplantation on white matter regeneration following spinal cord focal injury in adult rats". Experimental and Therapeutic Medicine 14.5 (2017): 4869-4877.
Chicago
Marcol, W., Ślusarczyk, W., Larysz‑Brysz, M., Łabuzek, K., Kapustka, B., Staszkiewicz, R., Rosicka, P., Kalita, K., Węglarz, W., Lewin‑Kowalik, J."Extended magnetic resonance imaging studies on the effect of classically activated microglia transplantation on white matter regeneration following spinal cord focal injury in adult rats". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4869-4877. https://doi.org/10.3892/etm.2017.5130
Copy and paste a formatted citation
x
Spandidos Publications style
Marcol W, Ślusarczyk W, Larysz‑Brysz M, Łabuzek K, Kapustka B, Staszkiewicz R, Rosicka P, Kalita K, Węglarz W, Lewin‑Kowalik J, Lewin‑Kowalik J, et al: Extended magnetic resonance imaging studies on the effect of classically activated microglia transplantation on white matter regeneration following spinal cord focal injury in adult rats. Exp Ther Med 14: 4869-4877, 2017.
APA
Marcol, W., Ślusarczyk, W., Larysz‑Brysz, M., Łabuzek, K., Kapustka, B., Staszkiewicz, R. ... Lewin‑Kowalik, J. (2017). Extended magnetic resonance imaging studies on the effect of classically activated microglia transplantation on white matter regeneration following spinal cord focal injury in adult rats. Experimental and Therapeutic Medicine, 14, 4869-4877. https://doi.org/10.3892/etm.2017.5130
MLA
Marcol, W., Ślusarczyk, W., Larysz‑Brysz, M., Łabuzek, K., Kapustka, B., Staszkiewicz, R., Rosicka, P., Kalita, K., Węglarz, W., Lewin‑Kowalik, J."Extended magnetic resonance imaging studies on the effect of classically activated microglia transplantation on white matter regeneration following spinal cord focal injury in adult rats". Experimental and Therapeutic Medicine 14.5 (2017): 4869-4877.
Chicago
Marcol, W., Ślusarczyk, W., Larysz‑Brysz, M., Łabuzek, K., Kapustka, B., Staszkiewicz, R., Rosicka, P., Kalita, K., Węglarz, W., Lewin‑Kowalik, J."Extended magnetic resonance imaging studies on the effect of classically activated microglia transplantation on white matter regeneration following spinal cord focal injury in adult rats". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4869-4877. https://doi.org/10.3892/etm.2017.5130
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