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Pigment epithelium‑derived factor short peptides facilitate full‑thickness cutaneous wound healing by promoting epithelial basal cell and hair follicle stem cell proliferation

  • Authors:
    • Shawn H. Tsai
    • Li‑Pen Tsao
    • Shih‑Hsin Chang
    • Tsung‑Chuan Ho
    • Kwang‑Yi Tung
    • Ai‑Ching Wu
    • Yeou‑Ping Tsao
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, Mackay Memorial Hospital, Taipei 10449, Taiwan, R.O.C., Department of Medical Research, Mackay Memorial Hospital, Taipei 10449, Taiwan, R.O.C., Department of Plastic Surgery, Mackay Memorial Hospital, Taipei 10449, Taiwan, R.O.C.
    Copyright: © Tsai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4853-4861
    |
    Published online on: September 19, 2017
       https://doi.org/10.3892/etm.2017.5134
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Abstract

Abstract. A previous study by our group showed that a 44‑amino‑acid fragment of pigment epithelium‑derived factor (PEDF) facilitated corneal epithelial wound healing. In the present study this fragment was shortened to obtain peptides of 18, 20 and 29 amino acids in length, and their promoting effects on the healing of full‑thickness skin wounds were assessed. Peptides were delivered periodically by topical application to punch wounds of mice. The wound healing speed was evaluated by measuring the reduction of wound areas at 4 and 7 days after injury. Histological analysis with Masson's trichrome staining was used to confirm epithelialization and dermal collagen deposition. Proliferation of epithelial basal cells was documented by 5‑bromo‑2'‑deoxyuridine incorporation. Hair follicle stem cells were identified by immunostaining for leucine‑rich repeat‑containing G protein‑coupled receptor 6. The results indicated that the 20‑ and 29‑amino‑acid short peptides significantly reduced the time required for wound healing compared to the vehicle. Histological analysis confirmed faster epithelial cell coverage of open wounds. Treatment with the PEDF peptide fragments also contributed to granulation, tissue formation by increasing the fibroblast population and enhancing collagen deposition in the dermis. Wounds treated with PEDF peptide fragments contained more basal cells proliferated in the epithelium. Moreover, hair follicle stem cells were also stimulated to proliferate by peptide exposure. In conclusion, the present study reported the identification of two short peptides that can enhance the healing of full‑thickness skin wounds following topical application. The underlying mechanisms may involve activation of basal cell proliferation and mobilization of hair follicle stem cells.
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Copy and paste a formatted citation
Spandidos Publications style
Tsai SH, Tsao LP, Chang SH, Ho TC, Tung KY, Wu AC and Tsao YP: Pigment epithelium‑derived factor short peptides facilitate full‑thickness cutaneous wound healing by promoting epithelial basal cell and hair follicle stem cell proliferation. Exp Ther Med 14: 4853-4861, 2017.
APA
Tsai, S.H., Tsao, L., Chang, S., Ho, T., Tung, K., Wu, A., & Tsao, Y. (2017). Pigment epithelium‑derived factor short peptides facilitate full‑thickness cutaneous wound healing by promoting epithelial basal cell and hair follicle stem cell proliferation. Experimental and Therapeutic Medicine, 14, 4853-4861. https://doi.org/10.3892/etm.2017.5134
MLA
Tsai, S. H., Tsao, L., Chang, S., Ho, T., Tung, K., Wu, A., Tsao, Y."Pigment epithelium‑derived factor short peptides facilitate full‑thickness cutaneous wound healing by promoting epithelial basal cell and hair follicle stem cell proliferation". Experimental and Therapeutic Medicine 14.5 (2017): 4853-4861.
Chicago
Tsai, S. H., Tsao, L., Chang, S., Ho, T., Tung, K., Wu, A., Tsao, Y."Pigment epithelium‑derived factor short peptides facilitate full‑thickness cutaneous wound healing by promoting epithelial basal cell and hair follicle stem cell proliferation". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4853-4861. https://doi.org/10.3892/etm.2017.5134
Copy and paste a formatted citation
x
Spandidos Publications style
Tsai SH, Tsao LP, Chang SH, Ho TC, Tung KY, Wu AC and Tsao YP: Pigment epithelium‑derived factor short peptides facilitate full‑thickness cutaneous wound healing by promoting epithelial basal cell and hair follicle stem cell proliferation. Exp Ther Med 14: 4853-4861, 2017.
APA
Tsai, S.H., Tsao, L., Chang, S., Ho, T., Tung, K., Wu, A., & Tsao, Y. (2017). Pigment epithelium‑derived factor short peptides facilitate full‑thickness cutaneous wound healing by promoting epithelial basal cell and hair follicle stem cell proliferation. Experimental and Therapeutic Medicine, 14, 4853-4861. https://doi.org/10.3892/etm.2017.5134
MLA
Tsai, S. H., Tsao, L., Chang, S., Ho, T., Tung, K., Wu, A., Tsao, Y."Pigment epithelium‑derived factor short peptides facilitate full‑thickness cutaneous wound healing by promoting epithelial basal cell and hair follicle stem cell proliferation". Experimental and Therapeutic Medicine 14.5 (2017): 4853-4861.
Chicago
Tsai, S. H., Tsao, L., Chang, S., Ho, T., Tung, K., Wu, A., Tsao, Y."Pigment epithelium‑derived factor short peptides facilitate full‑thickness cutaneous wound healing by promoting epithelial basal cell and hair follicle stem cell proliferation". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4853-4861. https://doi.org/10.3892/etm.2017.5134
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