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Article

Glucagon‑like peptide‑1 potentiates glucose‑stimulated insulin secretion via the transient receptor potential melastatin 2 channel

  • Authors:
    • Bo Pang
    • Sungjoon Kim
    • Daiqing Li
    • Zejun Ma
    • Bei Sun
    • Xiaona Zhang
    • Zhongming Wu
    • Liming Chen
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Hormones and Development (Ministry of Health), Tianjin Key Laboratory of Metabolic Diseases, Tianjin Metabolic Diseases Hospital and Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin 300070, P.R. China, Department of Physiology, Seoul National University College of Medicine, Seoul 110‑799, Republic of Korea
  • Pages: 5219-5227
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    Published online on: September 19, 2017
       https://doi.org/10.3892/etm.2017.5136
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Abstract

The transient receptor potential melastatin 2 (TRPM2) channel, a Ca2+ permeable channel activated by cAMP, is expressed on pancreatic β‑cells and is responsible for the regulation of insulin secretion. It is known that glucose‑stimulated insulin secretion (GSIS) can be potentiated by glucagon like peptide‑1 (GLP‑1), and that the changes in the extracellular glucose concentration alter the levels of intracellular adenosine ATP and cAMP. The present study hypothesized that TRPM2 mediates the modulatory effect of GLP‑1 on insulin secretion. The results demonstrated that silencing of TRPM2 eliminated GLP‑1‑enhanced insulin secretion, indicating the involvement of TRPM2 in this process. In addition, the results of current recordings of TRPM2 and measurement of the resulting insulin secretion in β‑cells in the presence of GLP‑1 and various concentrations of glucose suggest that GLP‑1 regulates GSIS via the TRPM2 channel. Furthermore, inhibiting the activity or expression of TRPM2 attenuated GLP‑1‑induced GSIS. By using specific activators or inhibitors, the present study demonstrated that the two primary downstream effectors of the GLP‑1 receptor, exchange protein directly activated by cAMP and protein kinase A, differentially influence GSIS and GLP‑1‑potentiated GSIS. In conclusion, the present study revealed the role of TRPM2 in GLP‑1‑regulated insulin secretion. The results of the present study provide a novel avenue for the prevention and treatment of diabetes and its complications.
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Copy and paste a formatted citation
Spandidos Publications style
Pang B, Kim S, Li D, Ma Z, Sun B, Zhang X, Wu Z and Chen L: Glucagon‑like peptide‑1 potentiates glucose‑stimulated insulin secretion via the transient receptor potential melastatin 2 channel. Exp Ther Med 14: 5219-5227, 2017.
APA
Pang, B., Kim, S., Li, D., Ma, Z., Sun, B., Zhang, X. ... Chen, L. (2017). Glucagon‑like peptide‑1 potentiates glucose‑stimulated insulin secretion via the transient receptor potential melastatin 2 channel. Experimental and Therapeutic Medicine, 14, 5219-5227. https://doi.org/10.3892/etm.2017.5136
MLA
Pang, B., Kim, S., Li, D., Ma, Z., Sun, B., Zhang, X., Wu, Z., Chen, L."Glucagon‑like peptide‑1 potentiates glucose‑stimulated insulin secretion via the transient receptor potential melastatin 2 channel". Experimental and Therapeutic Medicine 14.5 (2017): 5219-5227.
Chicago
Pang, B., Kim, S., Li, D., Ma, Z., Sun, B., Zhang, X., Wu, Z., Chen, L."Glucagon‑like peptide‑1 potentiates glucose‑stimulated insulin secretion via the transient receptor potential melastatin 2 channel". Experimental and Therapeutic Medicine 14, no. 5 (2017): 5219-5227. https://doi.org/10.3892/etm.2017.5136
Copy and paste a formatted citation
x
Spandidos Publications style
Pang B, Kim S, Li D, Ma Z, Sun B, Zhang X, Wu Z and Chen L: Glucagon‑like peptide‑1 potentiates glucose‑stimulated insulin secretion via the transient receptor potential melastatin 2 channel. Exp Ther Med 14: 5219-5227, 2017.
APA
Pang, B., Kim, S., Li, D., Ma, Z., Sun, B., Zhang, X. ... Chen, L. (2017). Glucagon‑like peptide‑1 potentiates glucose‑stimulated insulin secretion via the transient receptor potential melastatin 2 channel. Experimental and Therapeutic Medicine, 14, 5219-5227. https://doi.org/10.3892/etm.2017.5136
MLA
Pang, B., Kim, S., Li, D., Ma, Z., Sun, B., Zhang, X., Wu, Z., Chen, L."Glucagon‑like peptide‑1 potentiates glucose‑stimulated insulin secretion via the transient receptor potential melastatin 2 channel". Experimental and Therapeutic Medicine 14.5 (2017): 5219-5227.
Chicago
Pang, B., Kim, S., Li, D., Ma, Z., Sun, B., Zhang, X., Wu, Z., Chen, L."Glucagon‑like peptide‑1 potentiates glucose‑stimulated insulin secretion via the transient receptor potential melastatin 2 channel". Experimental and Therapeutic Medicine 14, no. 5 (2017): 5219-5227. https://doi.org/10.3892/etm.2017.5136
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