Glucagon‑like peptide‑1 potentiates glucose‑stimulated insulin secretion via the transient receptor potential melastatin 2 channel

  • Authors:
    • Bo Pang
    • Sungjoon Kim
    • Daiqing Li
    • Zejun Ma
    • Bei Sun
    • Xiaona Zhang
    • Zhongming Wu
    • Liming Chen
  • View Affiliations

  • Published online on: September 19, 2017     https://doi.org/10.3892/etm.2017.5136
  • Pages: 5219-5227
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Abstract

The transient receptor potential melastatin 2 (TRPM2) channel, a Ca2+ permeable channel activated by cAMP, is expressed on pancreatic β‑cells and is responsible for the regulation of insulin secretion. It is known that glucose‑stimulated insulin secretion (GSIS) can be potentiated by glucagon like peptide‑1 (GLP‑1), and that the changes in the extracellular glucose concentration alter the levels of intracellular adenosine ATP and cAMP. The present study hypothesized that TRPM2 mediates the modulatory effect of GLP‑1 on insulin secretion. The results demonstrated that silencing of TRPM2 eliminated GLP‑1‑enhanced insulin secretion, indicating the involvement of TRPM2 in this process. In addition, the results of current recordings of TRPM2 and measurement of the resulting insulin secretion in β‑cells in the presence of GLP‑1 and various concentrations of glucose suggest that GLP‑1 regulates GSIS via the TRPM2 channel. Furthermore, inhibiting the activity or expression of TRPM2 attenuated GLP‑1‑induced GSIS. By using specific activators or inhibitors, the present study demonstrated that the two primary downstream effectors of the GLP‑1 receptor, exchange protein directly activated by cAMP and protein kinase A, differentially influence GSIS and GLP‑1‑potentiated GSIS. In conclusion, the present study revealed the role of TRPM2 in GLP‑1‑regulated insulin secretion. The results of the present study provide a novel avenue for the prevention and treatment of diabetes and its complications.
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November-2017
Volume 14 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Pang B, Kim S, Li D, Ma Z, Sun B, Zhang X, Wu Z and Chen L: Glucagon‑like peptide‑1 potentiates glucose‑stimulated insulin secretion via the transient receptor potential melastatin 2 channel. Exp Ther Med 14: 5219-5227, 2017
APA
Pang, B., Kim, S., Li, D., Ma, Z., Sun, B., Zhang, X. ... Chen, L. (2017). Glucagon‑like peptide‑1 potentiates glucose‑stimulated insulin secretion via the transient receptor potential melastatin 2 channel. Experimental and Therapeutic Medicine, 14, 5219-5227. https://doi.org/10.3892/etm.2017.5136
MLA
Pang, B., Kim, S., Li, D., Ma, Z., Sun, B., Zhang, X., Wu, Z., Chen, L."Glucagon‑like peptide‑1 potentiates glucose‑stimulated insulin secretion via the transient receptor potential melastatin 2 channel". Experimental and Therapeutic Medicine 14.5 (2017): 5219-5227.
Chicago
Pang, B., Kim, S., Li, D., Ma, Z., Sun, B., Zhang, X., Wu, Z., Chen, L."Glucagon‑like peptide‑1 potentiates glucose‑stimulated insulin secretion via the transient receptor potential melastatin 2 channel". Experimental and Therapeutic Medicine 14, no. 5 (2017): 5219-5227. https://doi.org/10.3892/etm.2017.5136