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A rabbit model of implant‑related osteomyelitis inoculated with biofilm after open femoral fracture

  • Authors:
    • Xiang Zhang
    • Yun‑Fei Ma
    • Lei Wang
    • Nan Jiang
    • Cheng‑He Qin
    • Yan‑Jun Hu
    • Bin Yu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4995-5001
    |
    Published online on: September 19, 2017
       https://doi.org/10.3892/etm.2017.5138
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Abstract

Currently, animal models used in research on implant‑associated osteomyelitis primarily use intramedullary fixation and initial inoculum of planktonic bacterial cells. However, these techniques have certain limitations, including lack of rotational stability and instable inoculation. To improve these models, the present study aimed to establish a novel rabbit model of implant‑associated osteomyelitis using biofilm as the initial inoculum following plate fixation of the femoral fracture. A total of 24 New Zealand White rabbits were randomly divided into two equal groups. Osteotomy was performed at the right femoral shaft using a wire saw following fixation with a 5‑hole stainless steel plate. The plates were not colonized with bacteria in group 1, but colonized with a biofilm of Staphylococcus aureus (American Type Culture Collection, 25923) in group 2. All the rabbits were sacrificed after 21 days for clinical, X‑ray, micro‑computed tomography and histological assessments of the severity of osteomyelitis. Scanning electron microscopy and confocal laser scanning microscopy were used for biofilm assessment. In group 2, pus formation, periosteal reaction, cortical destruction and absorption were observed in all the rabbits and biofilm formation was observed on all the plates. However, no pus formation was observed except for a slight inflammatory response and all the plates appeared clean without infection in group 1. The differences between the two groups were statistically significant regarding histologic scores and semi‑quantification of the bacteria on the plates (P<0.001). In the present study, a novel rabbit model of infection following internal plate fixation of open fracture was successfully established, providing a novel tool for the study of implant‑associated osteomyelitis.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang X, Ma YF, Wang L, Jiang N, Qin CH, Hu YJ and Yu B: A rabbit model of implant‑related osteomyelitis inoculated with biofilm after open femoral fracture. Exp Ther Med 14: 4995-5001, 2017.
APA
Zhang, X., Ma, Y., Wang, L., Jiang, N., Qin, C., Hu, Y., & Yu, B. (2017). A rabbit model of implant‑related osteomyelitis inoculated with biofilm after open femoral fracture. Experimental and Therapeutic Medicine, 14, 4995-5001. https://doi.org/10.3892/etm.2017.5138
MLA
Zhang, X., Ma, Y., Wang, L., Jiang, N., Qin, C., Hu, Y., Yu, B."A rabbit model of implant‑related osteomyelitis inoculated with biofilm after open femoral fracture". Experimental and Therapeutic Medicine 14.5 (2017): 4995-5001.
Chicago
Zhang, X., Ma, Y., Wang, L., Jiang, N., Qin, C., Hu, Y., Yu, B."A rabbit model of implant‑related osteomyelitis inoculated with biofilm after open femoral fracture". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4995-5001. https://doi.org/10.3892/etm.2017.5138
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang X, Ma YF, Wang L, Jiang N, Qin CH, Hu YJ and Yu B: A rabbit model of implant‑related osteomyelitis inoculated with biofilm after open femoral fracture. Exp Ther Med 14: 4995-5001, 2017.
APA
Zhang, X., Ma, Y., Wang, L., Jiang, N., Qin, C., Hu, Y., & Yu, B. (2017). A rabbit model of implant‑related osteomyelitis inoculated with biofilm after open femoral fracture. Experimental and Therapeutic Medicine, 14, 4995-5001. https://doi.org/10.3892/etm.2017.5138
MLA
Zhang, X., Ma, Y., Wang, L., Jiang, N., Qin, C., Hu, Y., Yu, B."A rabbit model of implant‑related osteomyelitis inoculated with biofilm after open femoral fracture". Experimental and Therapeutic Medicine 14.5 (2017): 4995-5001.
Chicago
Zhang, X., Ma, Y., Wang, L., Jiang, N., Qin, C., Hu, Y., Yu, B."A rabbit model of implant‑related osteomyelitis inoculated with biofilm after open femoral fracture". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4995-5001. https://doi.org/10.3892/etm.2017.5138
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