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Topically administered rhGM-CSF affects PPARβ expression in the stasis zone

  • Authors:
    • Gen-Feng Fu
    • She-Min Tian
    • Xin-Jian Cha
    • Hong-Jun Huang
    • Ji-He Lou
    • Ying Wei
    • Cheng-De Xia
    • Yong-Lin Li
    • Xi-Hua Niu
  • View Affiliations / Copyright

    Affiliations: Department of Burn and Plastic Surgery, Taihe Hospital, Changsha, Hunan 410005, P.R. China, Department of Burns, the First People's Hospital of Zhengzhou City, Zhengzhou, Henan 450004, P.R. China
    Copyright: © Fu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4825-4830
    |
    Published online on: September 20, 2017
       https://doi.org/10.3892/etm.2017.5146
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Abstract

Using a rat comb thermal damage model, we investigated the effects of topically administered recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) on peroxisome proliferator-activated receptor PPARβ expression. We created bilateral comb scald models on the backs of fifty Sprague-Dawley rats. The left sides of the backs served as the experimental group and the right sides served as the control group. The experimental group received topically applied rhGM-CSF hydrogel and the control group did not. The survival situations of the stasis zones were compared between the experimental and control groups on the 1st, 3rd, 7th, 14th and 21st post-burn days. Tissues from the surviving stasis zones of both groups were collected at different time-points. Reverse transcriptase-polymerase chain reaction (RT-PCR) and western blotting were used to detect the PPARβ mRNA and protein expression levels. Immunohistochemical methods were applied to detect the localization of PPARβ protein expression. The results showed that, first, the tissue viability numbers for the stasis zones of the experimental group were significantly increased compared with those of the control group. Second, RT-PCR revealed that the PPARβ mRNA expression first increased and then gradually declined in both groups. At all time-points, the expression level in the experimental group was increased compared with that in the control group and the highest expression levels were observed in both groups on the 3rd post-burn day. Third, western blot analysis revealed that the PPARβ protein expression in both groups increased after thermal damage and then gradually decreased. PPARβ protein expression in the experimental group was greater than that in the control group, and the highest expression quantities in both groups were observed on the 3rd post-burn day. In conclusion, rhGM-CSF hydrogel effectively promotes the expression of PPARβ, and the hydrogel had a specific protective effect for the stasis zone.
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Copy and paste a formatted citation
Spandidos Publications style
Fu G, Tian S, Cha X, Huang H, Lou J, Wei Y, Xia C, Li Y and Niu X: Topically administered rhGM-CSF affects PPARβ expression in the stasis zone. Exp Ther Med 14: 4825-4830, 2017.
APA
Fu, G., Tian, S., Cha, X., Huang, H., Lou, J., Wei, Y. ... Niu, X. (2017). Topically administered rhGM-CSF affects PPARβ expression in the stasis zone. Experimental and Therapeutic Medicine, 14, 4825-4830. https://doi.org/10.3892/etm.2017.5146
MLA
Fu, G., Tian, S., Cha, X., Huang, H., Lou, J., Wei, Y., Xia, C., Li, Y., Niu, X."Topically administered rhGM-CSF affects PPARβ expression in the stasis zone". Experimental and Therapeutic Medicine 14.5 (2017): 4825-4830.
Chicago
Fu, G., Tian, S., Cha, X., Huang, H., Lou, J., Wei, Y., Xia, C., Li, Y., Niu, X."Topically administered rhGM-CSF affects PPARβ expression in the stasis zone". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4825-4830. https://doi.org/10.3892/etm.2017.5146
Copy and paste a formatted citation
x
Spandidos Publications style
Fu G, Tian S, Cha X, Huang H, Lou J, Wei Y, Xia C, Li Y and Niu X: Topically administered rhGM-CSF affects PPARβ expression in the stasis zone. Exp Ther Med 14: 4825-4830, 2017.
APA
Fu, G., Tian, S., Cha, X., Huang, H., Lou, J., Wei, Y. ... Niu, X. (2017). Topically administered rhGM-CSF affects PPARβ expression in the stasis zone. Experimental and Therapeutic Medicine, 14, 4825-4830. https://doi.org/10.3892/etm.2017.5146
MLA
Fu, G., Tian, S., Cha, X., Huang, H., Lou, J., Wei, Y., Xia, C., Li, Y., Niu, X."Topically administered rhGM-CSF affects PPARβ expression in the stasis zone". Experimental and Therapeutic Medicine 14.5 (2017): 4825-4830.
Chicago
Fu, G., Tian, S., Cha, X., Huang, H., Lou, J., Wei, Y., Xia, C., Li, Y., Niu, X."Topically administered rhGM-CSF affects PPARβ expression in the stasis zone". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4825-4830. https://doi.org/10.3892/etm.2017.5146
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