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Serological evaluation of antigen Tp0693 for diagnosis of syphilis

  • Authors:
    • Li Zhang
    • Meixia Deng
    • Xiaohong Zhang
    • Weiguo Yin
    • Wen Liu
    • Xi Luo
    • Jie Yang
    • Fei Chen
    • Feijun Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Laboratory, The First People's Hospital of Changde Affiliated to The University of South China, Changde, Hunan 415000, P.R. China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Institute of Pathogenic Biology, University of South China, Hengyang, Hunan 421001, P.R. China, Department of Laboratory, Yuebei People's Hospital, Shaoguan, Guangdong 512026, P.R. China, Department of Clinical Medicine, Medical College, University of South China, Hengyang, Hunan 421001, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4729-4736
    |
    Published online on: September 21, 2017
       https://doi.org/10.3892/etm.2017.5158
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Abstract

The aim of the present study was to evaluate the diagnostic value of the Treponema pallidum (Tp) antigen Tp0693 for syphilis. ELISA was used to examine the serum levels of Tp0693. The sample‑to‑cutoff ratio (S/CO) value was used to generate a receiver operating characteristic (ROC) curve. A correlation analysis was performed to compare the detection efficacy of Tp0693‑specific ELISA, Treponema pallidum Particle Agglutination (TPPA), Tolulized Red Unheated Serum test (TRUST) and LiZhu™ Tp‑ELISA. The area under the ROC curve was 0.99, indicating good diagnostic efficacy. When the diagnostic specificity reached 100%, the diagnostic sensitivity was up to 93.5%. Tp0693‑specific ELISA results were not correlated with those of TPPA, TRUST and LiZhu™ Tp‑ELISA (correlation coefficient, 0.122, 0.114 and 0.025, respectively). The latent syphilis rate was highest (12%, 9/75) for all syphilis specimens with a S/CO in the grey area. In conclusion, for syphilis specimens with a S/CO in the grey area, TPPA should be used for further confirmation of the diagnosis. Tp0693 may be used as a diagnostic antigen for syphilis; however, further study regarding its potential use is required.
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1 

Chadwick JA, MacNab A, Sarma J, Ray S, Kadir I and Muldoon EG: Secondary syphilis presenting with aortitis and coronary ostial occlusion. Sex Transm Infect. 92:108–109. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Sukthankar A: Syphilis. Medicine. 42:394–398. 2014. View Article : Google Scholar

3 

Stamm LV: Syphilis: Re-emergence of an old foe. Microb Cell. 3:363–370. 2016. View Article : Google Scholar : PubMed/NCBI

4 

World Health Organization, . Prevalence and incidence of selected sexually transmitted infections. Chlamydia trachomatis, Neisseria gonorrhoeae, syphilis and Trichomonas vaginalis: Methods and results used by WHO to generate 2005 estimates. World Health Organization; Geneva: 2011

5 

World Health Organization, . Global prevalence and incidence of selected curable sexually transmitted infections: Overview and estimates. World Health Organization; Geneva: 2001

6 

Tucker JD, Chen XS and Peeling RW: Syphilis and social upheaval in China. N Engl J Med. 362:1658–1661. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Tucker JD and Cohen MS: China's syphilis epidemic: Epidemiology, proximate determinants of spread, and control responses. Curr Opin Infect Dis. 24:50–55. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Klein J, McLaud M and Rogers D: Syphilis on the Rise: Diagnosis, treatment and prevention. J Nurse Pract. 11:49–55. 2015. View Article : Google Scholar

9 

Zanto SN: Changing algorithms in syphilis laboratory diagnosis. Clin Microbiol Newsl. 32:59–64. 2010. View Article : Google Scholar

10 

Workowski KA and Bolan GA: Centers for Disease Control and Prevention: Sexually transmitted diseases treatment guidelines, 2015. MMWR Recomm Rep. 64:1–137. 2015.PubMed/NCBI

11 

Morales-Múnera CE, Fuentes-Finkelstein PA and Vall Mayans M: Update on the diagnosis and treatment of RR-Sífilis: Actualización en el manejo diagnóstico y terapéutico. 106:1–69. 2015.

12 

Liu F, Liu LL, Guo XJ, Xi Y, Lin LR, Zhang HL, Huang SJ, Chen YY, Zhang YF, Zhang Q, et al: Characterization of the classical biological false-positive reaction in the serological test for syphilis in the modern era. Int Immunopharmacol. 20:331–336. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Eickhoff CA and Decker CF: Syphilis. Dis Mon. 62:280–286. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Sun AH, Mao YF, Hu Y, Sun Q and Yan J: Sensitive and specific ELISA coated by TpN15-TpN17-TpN47 fusion protein for detection of antibodies to Treponema pallidum. Clin Chem Lab Med. 47:321–326. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Moreno AI Brito, Bas C Acosta, Rodríguez M, Conde IB Baluja, Carballo S Feal and Martínez L: Monoclonal antibodies to the recombinant protein TmpA of the Treponema pallidum. Hybrid Hybridomics. 22:393–396. 2003. View Article : Google Scholar : PubMed/NCBI

16 

Baughn RE, Jiang A, Abraham R, Ottmers V and Musher DM: Molecular mimicry between an immunodominant amino acid motif on the 47-kDa lipoprotein of Treponema pallidum (Tpp47) and multiple repeats of analogous sequences in fibronectin. J Immunol. 157:720–731. 1996.PubMed/NCBI

17 

Lafond RE and Lukeart SA: Biological basis for syphilis. Clin Microbiol Rev. 19:29–49. 2006. View Article : Google Scholar : PubMed/NCBI

18 

McKevitt M, Brinkman MB, McLoughlin M, Perez C, Howell JK, Weinstock GM, Norris SJ and Palzkill T: Genome scale identification of Treponema pallidum antigens. Infect Immun. 73:4445–4450. 2005. View Article : Google Scholar : PubMed/NCBI

19 

Pinedo-Cancino V, Laurenti MD, Kesper N and Umezawa ES: Evaluation of Leishmania (Leishmania) infantum excreted-secretion antigens for detection of canine leishmaniasis. Acta Trop. 161:41–43. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Rizvi N, Singh A, Yadav M, Hussain SR, Siddiqui S, Kumar V, Ali S and Agarwal A: Role of alpha-crystallin, early-secretion antigenic target 6-kDa protein and culture filtrate protein 10 as novel diagnostic markers in osteoarticular tuberculosis. J Ortho Trans. 6:18–26. 2016.

21 

Sabry MA, Taher ES, Allah NF and Mahgoub AM: Diagnosis of Fasciola infection by SDS-PAGE eluted excretory secretory (ES) protein fractions using dot-ELISA. Int J Vet Sci Med. 2:130–135. 2014. View Article : Google Scholar

22 

Sánchez-Andrade R, Cortiñas FJ, Francisco I, Sánchez JA, Mula P, Cazapal C, Vázquez L, Suárez JL, Francisco R, Arias MS, et al: A novel second instar Gasterophilus excretory/secretory antigen-based ELISA for the diagnosis of gasterophilosis in grazing horses. Vet Parasitol. 171:314–320. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Xu M, Xie Y, Jiang C, Xiao Y, Kuang X, Zhao F, Zeng T, Liu S, Liang M, Li L, et al: A novel ELISA using a recombinant outer membrane protein, rTp0663, as the antigen for serological diagnosis of syphilis. Int J Infect Dis. 43:51–57. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Jiang C, Zhao F, Xiao J, Zeng T, Yu J, Ma X, Wu H and Wu Y: Evaluation of the recombinant protein TpF1 of Treponema pallidum for serodiagnosis of syphilis. Clin Vaccine Immunol. 20:1563–1568. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Jiang C, Xiao J, Xie Y, Xiao Y, Wang C, Kuang X, Xu M, Li R, Zeng T, Liu S, et al: Evaluation of FlaB1, FlaB2, FlaB3, and Tp0463 of Treponema pallidum for serodiagnosis of syphilis. Diagn Microbiol Infect Dis. 84:105–111. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Tiwari D, Haque S, Tiwari RP, Jawed A, Govender T and Kruger HG: Fast and efficient detection of tuberculosis antigens using liposome encapsulated secretory proteins of Mycobacterium tuberculosis. J Microbiol Immunol Infect. 50:189–198. 2017. View Article : Google Scholar : PubMed/NCBI

27 

Liu C, Ou Q, Chen H, Chen J, Lin S, Jiang L and Yang B: The diagnostic value and performance evaluation of five serological tests for the detection of Treponema pallidum. J Clin Lab Anal. 28:204–209. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Cantor AG, Pappas M, Daeges M and Nelson HD: Screening for Syphilis: Updated evidence report and systematic review for the US preventive services task force. JAMA. 315:2328–2337. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Yoshioka N, Deguchi M, Kagita M, Kita M, Watanabe M, Asari S and Iwatani Y: Evaluation of a chemiluminescent microparticle immunoassay for determination of Treponema pallidum antibodies. Clin Lab. 53:597–603. 2007.PubMed/NCBI

30 

Wang L, Deng X, Liu H, Zhao L, You X, Dai P, Wan K and Zeng Y: The mimic epitopes of Mycobacterium tuberculosis screened by phage display peptide library have serodiagnositic potential for tuberculosis. Pathog Dis. 74:pii: ftw0912016. View Article : Google Scholar

31 

Bosshard PP: Usefulness of IgM-specific enzyme immunoassays for serodiagnosis of syphilis: Comparative evaluation of three different assays. J Infect. 67:35–42. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Herremans M, Notermans DW, Mommers M and Kortbeek LM: Comparison of a Treponema pallidum IgM immunoblot with a 19S fluorescent treponemal antibody absorption test for the diagnosis of congenital syphilis. Diagn Microbiol Infect Dis. 59:61–66. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang L, Deng M, Zhang X, Yin W, Liu W, Luo X, Yang J, Chen F and Zhao F: Serological evaluation of antigen Tp0693 for diagnosis of syphilis. Exp Ther Med 14: 4729-4736, 2017.
APA
Zhang, L., Deng, M., Zhang, X., Yin, W., Liu, W., Luo, X. ... Zhao, F. (2017). Serological evaluation of antigen Tp0693 for diagnosis of syphilis. Experimental and Therapeutic Medicine, 14, 4729-4736. https://doi.org/10.3892/etm.2017.5158
MLA
Zhang, L., Deng, M., Zhang, X., Yin, W., Liu, W., Luo, X., Yang, J., Chen, F., Zhao, F."Serological evaluation of antigen Tp0693 for diagnosis of syphilis". Experimental and Therapeutic Medicine 14.5 (2017): 4729-4736.
Chicago
Zhang, L., Deng, M., Zhang, X., Yin, W., Liu, W., Luo, X., Yang, J., Chen, F., Zhao, F."Serological evaluation of antigen Tp0693 for diagnosis of syphilis". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4729-4736. https://doi.org/10.3892/etm.2017.5158
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang L, Deng M, Zhang X, Yin W, Liu W, Luo X, Yang J, Chen F and Zhao F: Serological evaluation of antigen Tp0693 for diagnosis of syphilis. Exp Ther Med 14: 4729-4736, 2017.
APA
Zhang, L., Deng, M., Zhang, X., Yin, W., Liu, W., Luo, X. ... Zhao, F. (2017). Serological evaluation of antigen Tp0693 for diagnosis of syphilis. Experimental and Therapeutic Medicine, 14, 4729-4736. https://doi.org/10.3892/etm.2017.5158
MLA
Zhang, L., Deng, M., Zhang, X., Yin, W., Liu, W., Luo, X., Yang, J., Chen, F., Zhao, F."Serological evaluation of antigen Tp0693 for diagnosis of syphilis". Experimental and Therapeutic Medicine 14.5 (2017): 4729-4736.
Chicago
Zhang, L., Deng, M., Zhang, X., Yin, W., Liu, W., Luo, X., Yang, J., Chen, F., Zhao, F."Serological evaluation of antigen Tp0693 for diagnosis of syphilis". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4729-4736. https://doi.org/10.3892/etm.2017.5158
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