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Electroacupuncture improves acute bowel injury recovery in rat models

  • Authors:
    • Jiannong Wu
    • Bin Lyu
    • Tie'er Gan
    • Lingcong Wang
    • Meifei Zhu
  • View Affiliations / Copyright

    Affiliations: Department of Critical Care, The First Affiliated Hospital, Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang 310053, P.R. China, Division of Gastroenterology, The First Affiliated Hospital, Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang 310053, P.R. China, Department of Infectious Diseases, The First Affiliated Hospital, Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang 310053, P.R. China
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4655-4662
    |
    Published online on: September 21, 2017
       https://doi.org/10.3892/etm.2017.5159
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Abstract

Electroacupuncture (EA) accelerates intestinal functional recovery in sepsis. The present study investigated ghrelin and ghrelin receptor (GSH‑R) levels during EA in rats with acute bowel injury (ABI). Rats were grouped into four groups: Sham, ABI, ABI+EA and ABI+GHRA+EA (n=12 per group). ABI was induced by cecal ligation and puncture (CLP). EA on bilateral Zusanli acupoints was performed following CLP. GSH‑R blocker (GHRA) was used following CLP but prior to EA for ABI+GHRA+EA rats. Rats were sacrificed 12 h following CLP. Serum ghrelin, tumor necrosis factor‑α (TNF‑α) and high mobility group box 1 (HMGB1) levels, as well as ghrelin and GSH‑R protein expression, water content, pathological changes and myeloperoxidase (MPO) and diamine oxidase (DAO) activities in the bowel tissues, were measured. ABI rats, compared with the sham rats, had significantly lower levels of ghrelin and GSH‑R in the serum and bowel tissue, and higher Chiu's score (all P<0.05). The ABI+EA rats, compared with the ABI rats, had significantly reduced serum TNF‑α and HMGB1 levels, bowel water content, MPO activity and Chiu's score (all P<0.05), and significantly higher serum ghrelin (121.2±10.7 vs. 86.7±6.4 pg/ml), bowel ghrelin (0.12±0.02 vs. 0.08±0.01), GSH‑R (0.05±0.04 vs. 0.03±0.01) and DAO activity (18.74±4.18 vs. 13.52±2.33 U/ml; all P<0.05), indicating an improvement of the intestinal mucosal barrier. GHRA reversed the protective effects of EA. Therefore, EA improved ABI recovery by promoting ghrelin secretion and upregulating GSH‑R expression.
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Copy and paste a formatted citation
Spandidos Publications style
Wu J, Lyu B, Gan T, Wang L and Zhu M: Electroacupuncture improves acute bowel injury recovery in rat models. Exp Ther Med 14: 4655-4662, 2017.
APA
Wu, J., Lyu, B., Gan, T., Wang, L., & Zhu, M. (2017). Electroacupuncture improves acute bowel injury recovery in rat models. Experimental and Therapeutic Medicine, 14, 4655-4662. https://doi.org/10.3892/etm.2017.5159
MLA
Wu, J., Lyu, B., Gan, T., Wang, L., Zhu, M."Electroacupuncture improves acute bowel injury recovery in rat models". Experimental and Therapeutic Medicine 14.5 (2017): 4655-4662.
Chicago
Wu, J., Lyu, B., Gan, T., Wang, L., Zhu, M."Electroacupuncture improves acute bowel injury recovery in rat models". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4655-4662. https://doi.org/10.3892/etm.2017.5159
Copy and paste a formatted citation
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Spandidos Publications style
Wu J, Lyu B, Gan T, Wang L and Zhu M: Electroacupuncture improves acute bowel injury recovery in rat models. Exp Ther Med 14: 4655-4662, 2017.
APA
Wu, J., Lyu, B., Gan, T., Wang, L., & Zhu, M. (2017). Electroacupuncture improves acute bowel injury recovery in rat models. Experimental and Therapeutic Medicine, 14, 4655-4662. https://doi.org/10.3892/etm.2017.5159
MLA
Wu, J., Lyu, B., Gan, T., Wang, L., Zhu, M."Electroacupuncture improves acute bowel injury recovery in rat models". Experimental and Therapeutic Medicine 14.5 (2017): 4655-4662.
Chicago
Wu, J., Lyu, B., Gan, T., Wang, L., Zhu, M."Electroacupuncture improves acute bowel injury recovery in rat models". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4655-4662. https://doi.org/10.3892/etm.2017.5159
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