Electroacupuncture improves acute bowel injury recovery in rat models

Wu,Jiannong; Lyu,Bin; Gan,Tie'er; Wang,Lingcong; Zhu,Meifei

doi:10.3892/etm.2017.5159

Electroacupuncture improves acute bowel injury recovery in rat models

Authors:
- Jiannong Wu
- Bin Lyu
- Tie'er Gan
- Lingcong Wang
- Meifei Zhu
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Affiliations: Department of Critical Care, The First Affiliated Hospital, Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang 310053, P.R. China, Division of Gastroenterology, The First Affiliated Hospital, Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang 310053, P.R. China, Department of Infectious Diseases, The First Affiliated Hospital, Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang 310053, P.R. China
Published online on: September 21, 2017 https://doi.org/10.3892/etm.2017.5159
Pages: 4655-4662
Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Electroacupuncture (EA) accelerates intestinal functional recovery in sepsis. The present study investigated ghrelin and ghrelin receptor (GSH‑R) levels during EA in rats with acute bowel injury (ABI). Rats were grouped into four groups: Sham, ABI, ABI+EA and ABI+GHRA+EA (n=12 per group). ABI was induced by cecal ligation and puncture (CLP). EA on bilateral Zusanli acupoints was performed following CLP. GSH‑R blocker (GHRA) was used following CLP but prior to EA for ABI+GHRA+EA rats. Rats were sacrificed 12 h following CLP. Serum ghrelin, tumor necrosis factor‑α (TNF‑α) and high mobility group box 1 (HMGB1) levels, as well as ghrelin and GSH‑R protein expression, water content, pathological changes and myeloperoxidase (MPO) and diamine oxidase (DAO) activities in the bowel tissues, were measured. ABI rats, compared with the sham rats, had significantly lower levels of ghrelin and GSH‑R in the serum and bowel tissue, and higher Chiu's score (all P<0.05). The ABI+EA rats, compared with the ABI rats, had significantly reduced serum TNF‑α and HMGB1 levels, bowel water content, MPO activity and Chiu's score (all P<0.05), and significantly higher serum ghrelin (121.2±10.7 vs. 86.7±6.4 pg/ml), bowel ghrelin (0.12±0.02 vs. 0.08±0.01), GSH‑R (0.05±0.04 vs. 0.03±0.01) and DAO activity (18.74±4.18 vs. 13.52±2.33 U/ml; all P<0.05), indicating an improvement of the intestinal mucosal barrier. GHRA reversed the protective effects of EA. Therefore, EA improved ABI recovery by promoting ghrelin secretion and upregulating GSH‑R expression.

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1	Malbrain ML and De Laet I: AIDS is coming to your ICU: Be prepared for acute bowel injury and acute intestinal distress syndrome. Intensive Care Med. 34:1565–1569. 2008. View Article : Google Scholar : PubMed/NCBI
2	De-Souza DA and Greene LJ: Intestinal permeability and systemic infections in critically ill patients: Effect of glutamine. Crit Care Med. 33:1125–1135. 2005. View Article : Google Scholar : PubMed/NCBI
3	Seoane L, Winterbottom F, Nash T, Behrhorst J, Chacko E, Shum L, Pavlov A, Briski D, Thibeau S, Bergeron D, Rafael T and Sundell E: Using quality improvement principles to improve the care of patients with severe sepsis and septic shock. Ochsner J. 13:359–366. 2013.PubMed/NCBI
4	Hu S, Song Q, Wang HB, Lü Y, Yu Y, Wang L, Zhou GY, Shi X and Sheng ZY: Study on the protective effect and mechanism of electroacupuncturing at Zusanli point (ST 36) on endotoxin induced hepatic injury in rats. Zhong Guo Zhong Xi Yi Jie He Ji Jiu Za Zhi Bian Ji Bu. 14:296–298. 2007.(In Chinese).
5	Song Q, Hu S, Wang H, Lv Y, Shi X, Sheng Z and Sheng W: Electroacupuncturing at Zusanli point (ST36) attenuates pro-inflammatory cytokine release and organ dysfunction by activating cholinergic anti-inflammatory pathway in rat with endotoxin challenge. Afr J Tradit Complement Altern Med. 11:469–474. 2014. View Article : Google Scholar : PubMed/NCBI
6	Song XM, Li JG, Wang YL, Hu ZF, Zhou Q, Du ZH and Jia BH: The protective effect of the cholinergic anti-inflammatory pathway against septic shock in rats. Shock. 30:468–472. 2008. View Article : Google Scholar : PubMed/NCBI
7	Wang LC and Wu JN: The clinical efficacy of sepsis with early intervention of electroacupuncture. Life Sci J. 10:1900–1903. 2013.
8	Das UN: Relationship between gut and sepsis: Role of ghrelin. World J Diabetes. 2:1–7. 2011. View Article : Google Scholar : PubMed/NCBI
9	Wu R, Dong W, Qiang X, Wang H, Blau SA, Ravikumar TS and Wang P: Orexigenic hormone ghrelin ameliorates gut barrier dysfunction in sepsis in rats. Crit Care Med. 37:2421–2426. 2009. View Article : Google Scholar : PubMed/NCBI
10	Kojima M and Kangawa K: Ghrelin: Structure and function. Physiol Rev. 85:495–522. 2005. View Article : Google Scholar : PubMed/NCBI
11	Li WG, Gavrila D, Liu X, Wang L, Gunnlaugsson S, Stoll LL, McCormick ML, Sigmund CD, Tang C and Weintraub NL: Ghrelin inhibits proinflammatory responses and nuclear factor-kappaB activation in human endothelial cells. Circulation. 109:2221–2226. 2004. View Article : Google Scholar : PubMed/NCBI
12	Koch A, Sanson E, Helm A, Voigt S, Trautwein C and Tacke F: Regulation and prognostic relevance of serum ghrelin concentrations in critical illness and sepsis. Crit Care. 14:R942010. View Article : Google Scholar : PubMed/NCBI
13	Chaudry IH, Tabata Y, Schleck S and Baue AE: Effect of splenectomy on reticuloendothelial function and survival following sepsis. J Trauma. 20:649–656. 1980. View Article : Google Scholar : PubMed/NCBI
14	Rittirsch D, Huber-Lang MS, Flierl MA and Ward PA: Immunodesign of experimental sepsis by cecal ligation and puncture. Nat Protoc. 4:31–36. 2009. View Article : Google Scholar : PubMed/NCBI
15	Bao J, Tan S, Yu W, Lin Z, Dong Y, Chen Q, Shi J, Duan K, Bai X, Xu L, et al: The effect of peritoneal air exposure on intestinal mucosal barrier. Gastroenterol Res Pract. 2014:6748752014. View Article : Google Scholar : PubMed/NCBI
16	Chiu CJ, Mcardle AH, Brown R, Scott HJ and Gurd FN: Intestinal mucosal lesions in low flow states. I. A morphological, hemodynamic and metabolic reappraisal. Arch Surg. 101:478–483. 1970. View Article : Google Scholar : PubMed/NCBI
17	Longa EZ, Weinstein PR, Carlson S and Cummins R: Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke. 20:84–91. 1989. View Article : Google Scholar : PubMed/NCBI
18	Surbatovic M, Veljovic M, Jevdjic J, Popovic N, Djordjevic D and Radakovic S: Immunoinflammatory response in critically ill patients: Severe sepsis and/or trauma. Mediators Inflamm. 2013:3627932013. View Article : Google Scholar : PubMed/NCBI
19	Palone F, Vitali R, Cucchiara S, Pierdomenico M, Negroni A, Aloi M, Nuti F, Felice C, Armuzzi A and Stronati L: Role of HMGB1 as a suitable biomarker of subclinical intestinal inflammation and mucosal healing in patients with inflammatory bowel disease. Inflamm Bowel Dis. 20:1448–1457. 2014. View Article : Google Scholar : PubMed/NCBI
20	Zhao L, Luo L, Jia W, Xiao J, Huang G, Tian G, Li J and Xiao Y: Serum diamine oxidase as a hemorrhagic shock biomarker in a rabbit model. PLoS One. 9:e1022852014. View Article : Google Scholar : PubMed/NCBI
21	Mondello S, Galuppo M, Mazzon E, Domenico I, Mondello P, Carmela A and Cuzzocrea S: Glutamine treatment attenuates the development of ischaemia/reperfusion injury of the gut. Eur J Pharmacol. 643:304–315. 2010. View Article : Google Scholar : PubMed/NCBI
22	Guo H, Zhu SF, Zhang RR, Zhao XL, Wan MH and Tang WF: Electroacupuncture ameliorates acute lung injury through promoting gastrointestinal motility in rats with acute pancreatitis. Evid Based Complement Alternat Med. 2014:9435962014. View Article : Google Scholar : PubMed/NCBI
23	Wu HG, Jiang B, Zhou EH, Shi Z, Shi DR, Cui YH, Kou ST and Liu HR: Regulatory mechanism of electroacupuncture in irritable bowel syndrome: Preventing MC activation and decreasing SP VIP secretion. Dig Dis Sci. 53:1644–1651. 2008. View Article : Google Scholar : PubMed/NCBI
24	Lin YP, Yi SX, Yan J and Chang XR: Effect of acupuncture at Foot-Yangming Meridian on gastric mucosal blood flow, gastric motility and brain-gut peptide. World J Gastroenterol. 13:2229–2233. 2007. View Article : Google Scholar : PubMed/NCBI
25	Cheyuo C, Jacob A and Wang P: Ghrelin-mediated sympathoinhibition and suppression of inflammation in sepsis. Am J Physiol Endocrinol Metab. 302:E265–E272. 2012. View Article : Google Scholar : PubMed/NCBI
26	Jacob A, Wu R, Zhou M, Coppa GF and Wang P: Mechanism of the inhibitory effect of ghrelin in sepsis. Hepat Med. 2:33–38. 2010.PubMed/NCBI
27	Neto A Serpa, Veelo DP, Peireira VG, de Assunção MS, Manetta JA, Espósito DC and Schultz MJ: Fluid resuscitation with hydroxyethyl starches in patients with sepsis is associated with an increased incidence of acute kidney injury and use of renal replacement therapy: A systematic review and meta-analysis of the literature. J Crit Care. 185.e1-72014.PubMed/NCBI
28	Arnes L, Hill JT, Gross S, Magnuson MA and Sussel L: Ghrelin expression in the mouse pancreas defines a unique multipotent progenitor population. PLoS One. 7:e520262012. View Article : Google Scholar : PubMed/NCBI
29	Wu R, Dong W, Zhou M, Cui X, Simms H Hank and Wang P: Ghrelin improves tissue perfusion in severe sepsis via downregulation of endothelin-1. Cardiovasc Res. 68:318–326. 2005. View Article : Google Scholar : PubMed/NCBI
30	Wu R, Dong W, Zhou M, Zhang F, Marini CP, Ravikumar TS and Wang P: Ghrelin attenuates sepsis-induced acute lung injury and mortality in rats. Am J Respir Crit Care Med. 176:805–813. 2007. View Article : Google Scholar : PubMed/NCBI