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Article

Efficacy, safety and influencing factors of intra‑calf muscular injection of bone marrow mononuclear cells in the treatment of type 2 diabetes mellitus‑induced lower extremity vascular disease

  • Authors:
    • Hui‑Min Zhou
    • Fan Liu
    • Ai‑Ge Yang
    • Yu‑Qing Guo
    • Ya‑Ru Zhou
    • Yong‑Quan Gu
    • Bao‑Yong Yan
    • Quan‑Hai Li
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei 050031, P.R. China, Department of Endocrinology, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China, Department of Vascular Surgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, P.R. China, Cell Therapy Laboratory, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei 050031, P.R. China
  • Pages: 5177-5185
    |
    Published online on: September 22, 2017
       https://doi.org/10.3892/etm.2017.5193
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Abstract

The efficacy, safety and impact of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) associated with the intra‑calf muscular injection of bone marrow mononuclear cells (BMMCs) in the treatment of type 2 diabetes mellitus (T2DM)‑induced lower extremity vascular disease (LEVD) were evaluated. Patients with T2DM‑LEVD were randomly divided into a control group and BMMCs group to assess the efficacy and safety of the treatment; serum VEGF and bFGF levels were detected. The BMMCs group was divided into superior genicular artery (SGA) and inferior genicular artery (IGA) subgroups as well as low‑dose and high‑dose subgroups for the comparison of efficacy indices. The BMMCs group exhibited significantly improved indices (P<0.05) compared with the control group and no fatalities or cancer occurred. There were no significant changes in serum VEGF and bFGF levels (P>0.05). The claudication distance in the IGA subgroup was significantly greater that in the SGA subgroup (P<0.05); the low‑dose subgroup and the high‑dose subgroup did not demonstrate any significant differences in each index (P>0.05). BMMC treatment for T2DM‑LEVD was found to be safe and effective and had no significant impact on serum VEGF and bFGF levels in the short term; However, the degree of LEVD may affect its efficacy.
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1 

Shearman CP and Windhaber R: Foot complications in patients with diabetes. Surgery. 28:288–292. 2010.

2 

Dieter RS, Chu WW, Pacanowski JP Jr, McBride PE and Tanke TE: The significance of lower extremity peripheral arterial disease. Clin Cardiol. 25:3–10. 2002. View Article : Google Scholar : PubMed/NCBI

3 

Calne RY, Gan SU and Lee KO: Stem cell and gene therapies for diabetes mellitus. Nat Rev Endocrinol. 6:173–177. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Dequach JA, Lin JE, Cam C, Hu D, Salvatore MA, Sheikh F and Christman KL: Injectable skeletal muscle matrix hydrogel promotes neovascularization and muscle cell infiltration in a hindlimb ischemia model. Eur Cell Mater. 23:400–412. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Huang PP, Yang XF, Li SZ, Wen JC, Zhang Y and Han ZC: Randomised comparison of G-CSF-mobilized peripheral blood mononuclear cells versus bone marrow-mononuclear cells for the treatment of patients with lower limb arteriosclerosis obliterans. Thromb Haemost. 98:1335–1342. 2007.PubMed/NCBI

6 

Waksman R and Baffour R: Bone marrow and bone marrow derived mononuclear stem cells therapy for the chronically ischemic myocardium. Cardiovasc Radiat Med. 4:164–168. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Hayashida K, Fujita J, Miyake Y, Kawada H, Ando K, Ogawa S and Fukuda K: Bone marrow-derived cells contribute to pulmonary vascular remodeling in hypoxia-induced pulmonary hypertension. Chest. 127:1793–1798. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Tateishi-Yuyama E, Matsubara H, Murohara T, Ikeda U, Shintani S, Masaki H, Amano K, Kishimoto Y, Yoshimoto K, Akashi H, et al: Therapeutic angiogenesis for patients with limb ischaemia by autologous transplantation of bone-marrow cells: A pilot study and a randomised controlled trial. Lancet. 360:427–435. 2002. View Article : Google Scholar : PubMed/NCBI

9 

Furth ME and Atala A: Stem cell sources to treat diabetes. J Cell Biochem. 106:507–511. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Todd JA: Stem cells and a cure for type 1 diabetes? Proc Natl Acad Sci USA. 106:pp. 15523–15524. 2009; View Article : Google Scholar : PubMed/NCBI

11 

Urbán VS, Kiss J, Kovács J, Gócza E, Vas V, Monostori E and Uher F: Mesenchymal stem cells cooperate with bone marrow cells in therapy of diabetes. Stem Cells. 26:244–253. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Dubský M, Jirkovská A, Bém R, Pagácová L, Fejfarová V, Varga M, Skibová J, Langkramer S and Syková E: Treatment of critical limb ischemia and diabetic foot disease by the use of autologous stem cells. Vnitr Lek. 57:451–455. 2011.(In Czech). PubMed/NCBI

13 

Kirana S, Stratmann B, Prante C, Prohaska W, Koerperich H, Lammers D, Gastens MH, Quast T, Negrean M, Stirban OA, et al: Autologous stem cell therapy in the treatment of limb ischaemia induced chronic tissue ulcers of diabetic foot patients. Int J Clin Pract. 66:384–393. 2012. View Article : Google Scholar : PubMed/NCBI

14 

De Haro J, Acin F, Lopez-Quintana A, Florez A, Martinez-Aguilar E and Varela C: Meta-analysis of randomized, controlled clinical trials in angiogenesis: Gene and cell therapy inperipheral arterial disease. Heart Vessels. 24:321–328. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Hirata K, Li TS, Nishida M, Ito H, Matsuzaki M, Kasaoka S and Hamano K: Autologous bone marrow cell implantation as therapeutic angiogenesis for ischemic hindlimb in diabetic rat model. Am J Physiol Heart Circ Physiol. 284:H66–H70. 2003. View Article : Google Scholar : PubMed/NCBI

16 

Lee KB, Choi J, Cho SB, Chung JY, Moon ES, Kim NS and Han HJ: Topical embryonic stem cells enhance wound healing in diabetic rats. J Orthop Res. 29:1554–1562. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Park SJ, Moon SH, Lee HJ, Lim JJ, Kim JM, Seo J, Yoo JW, Kim OJ, Kang SW and Chung HM: A comparison of human cord blood- and embryonic stem cell-derived endothelial progenitor cells in the treatment of chronic wounds. Biomaterials. 34:995–1003. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Shin L and Peterson DA: Human mesenchymal stem cell grafts enhance normal and impaired wound healing by recruiting existing endogenous tissue stem/progenitor cells. Stem Cells Transl Med. 2:33–42. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Nakayama Y, Iwahana M, Sakamoto N, Tanaka NG and Osada Y: Inhibitory effects of a bacteria-derived sulfated polysaccharide against basic fibroblast growth factor-induced endothelial cell growth and chemotaxis. J Cell Physiol. 154:1–6. 1993. View Article : Google Scholar : PubMed/NCBI

20 

Leask A: Potential therapeutic targets for cardiac fibrosis: TGFbeta, angiotensin, endothelin, CCN2 and PDGF, partners in fibroblast activation. Circ Res. 106:1675–1680. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Lott JA and Turner K: Evaluation of Trinder's glucose oxidase method for measuring glucose in serum and urine. Clin Chem. 21:1754–1760. 1975.PubMed/NCBI

22 

Zhang Q, Xiao X, Feng K, Wang T, Li W, Yuan T, Sun X, Sun Q, Xiang H and Wang H: Berberine moderates glucose and lipid metabolism through multipathway mechanism. Evid Based Complement Alternat Med 2011. pii:9248512011.

23 

Schnedl WJ, Krause R, Halwachs-Baumann G, Trinker M, Lipp RW and Krejs GJ: Evaluation of HbA1c determination methods in patients with hemoglobinopathies. Diabetes Care. 23:339–344. 2000. View Article : Google Scholar : PubMed/NCBI

24 

Sun L, Zhou X, Zhang TL, Zhang G, Zuo M, Li J, Huang G, Lu D, Guang X and Yang D: Isolation, labeling and culture of bone marrow-derived mononuclear cells. Chin J Cardiovasc Rev. 777–779. 2008.(In Chinese).

25 

Gu YQ, Zhang J, Guo LR, Qi LX, Zhang SW, Xu J, Li JX, Luo T, Ji BX, Li XF, et al: Transplantation of autologous bone marrow mononuclear cells for patients with lower limb ischemia. Chin Med J (Engl). 121:963–967. 2008.PubMed/NCBI

26 

Das AK: Stem cell therapy for critical limb ischaemia-a review. Indian J Surg. 71:177–181. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Benoit E, O'Donnell TF and Patel AN: Safety and efficacy of autologous cell therapy in critical limb ischemia: A systematic review. Cell Transplant. 22:545–562. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Koleva-Georgieva DN, Sivkova NP and Terzieva D: Serum inflammatory cytokines IL-1beta, IL-6, TNF-alpha and VEGF have influence on the development of diabetic retinopathy. Folia Med (Plovdiv). 53:44–50. 2011.PubMed/NCBI

29 

Kaul K, Hodgkinson A, Tarr JM, Kohner EM and Chibber R: Is inflammation a common retinal-renal-nerve pathogenic link in diabetes? Curr Diabetes Rev. 6:294–303. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Carranza K and Veron D, Cercado A, Bautista N, Pozo W, Tufro A and Veron D: Cellular and molecular aspects of diabetic nephropathy; the role of VEGF-A. Nefrologia. 35:131–138. 2015.(In English, Spanish). View Article : Google Scholar : PubMed/NCBI

31 

Dabhi B, Mistry KN, Patel H and Lal S: Vascular endothelial growth factor insertion/deletion gene polymorphism in West Indian patients of type 2 diabetes and diabetic nephropathy. Indian J Biochem Biophys. 52:209–212. 2015.PubMed/NCBI

32 

Hatsukami TS, Primozich J, Zierler RE and Strandness DE Jr: Color Doppler characteristics in normal lower extremity arteries. Ultrasound Med Biol. 18:167–171. 1992. View Article : Google Scholar : PubMed/NCBI

33 

Tan O, Yuce I, Kantarci M and Algan S: Evaluation of lower-limb arteries with multidetector computed tomography angiography prior to free flap surgery: A radioanatomic study. J Reconstr Microsurg. 27:199–206. 2011. View Article : Google Scholar : PubMed/NCBI

34 

Graziani L, Silvestro A, Bertone V, Manara E, Andreini R, Sigala A, Mingardi R and De Giglio R: Vascular involvement in diabetic subjects with ischemic foot ulcer: A new morphologic categorization of disease severity. Eur J Vasc Endovasc Surg. 33:453–460. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhou HM, Liu F, Yang AG, Guo YQ, Zhou YR, Gu YQ, Yan BY and Li QH: Efficacy, safety and influencing factors of intra‑calf muscular injection of bone marrow mononuclear cells in the treatment of type 2 diabetes mellitus‑induced lower extremity vascular disease. Exp Ther Med 14: 5177-5185, 2017.
APA
Zhou, H., Liu, F., Yang, A., Guo, Y., Zhou, Y., Gu, Y. ... Li, Q. (2017). Efficacy, safety and influencing factors of intra‑calf muscular injection of bone marrow mononuclear cells in the treatment of type 2 diabetes mellitus‑induced lower extremity vascular disease. Experimental and Therapeutic Medicine, 14, 5177-5185. https://doi.org/10.3892/etm.2017.5193
MLA
Zhou, H., Liu, F., Yang, A., Guo, Y., Zhou, Y., Gu, Y., Yan, B., Li, Q."Efficacy, safety and influencing factors of intra‑calf muscular injection of bone marrow mononuclear cells in the treatment of type 2 diabetes mellitus‑induced lower extremity vascular disease". Experimental and Therapeutic Medicine 14.5 (2017): 5177-5185.
Chicago
Zhou, H., Liu, F., Yang, A., Guo, Y., Zhou, Y., Gu, Y., Yan, B., Li, Q."Efficacy, safety and influencing factors of intra‑calf muscular injection of bone marrow mononuclear cells in the treatment of type 2 diabetes mellitus‑induced lower extremity vascular disease". Experimental and Therapeutic Medicine 14, no. 5 (2017): 5177-5185. https://doi.org/10.3892/etm.2017.5193
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou HM, Liu F, Yang AG, Guo YQ, Zhou YR, Gu YQ, Yan BY and Li QH: Efficacy, safety and influencing factors of intra‑calf muscular injection of bone marrow mononuclear cells in the treatment of type 2 diabetes mellitus‑induced lower extremity vascular disease. Exp Ther Med 14: 5177-5185, 2017.
APA
Zhou, H., Liu, F., Yang, A., Guo, Y., Zhou, Y., Gu, Y. ... Li, Q. (2017). Efficacy, safety and influencing factors of intra‑calf muscular injection of bone marrow mononuclear cells in the treatment of type 2 diabetes mellitus‑induced lower extremity vascular disease. Experimental and Therapeutic Medicine, 14, 5177-5185. https://doi.org/10.3892/etm.2017.5193
MLA
Zhou, H., Liu, F., Yang, A., Guo, Y., Zhou, Y., Gu, Y., Yan, B., Li, Q."Efficacy, safety and influencing factors of intra‑calf muscular injection of bone marrow mononuclear cells in the treatment of type 2 diabetes mellitus‑induced lower extremity vascular disease". Experimental and Therapeutic Medicine 14.5 (2017): 5177-5185.
Chicago
Zhou, H., Liu, F., Yang, A., Guo, Y., Zhou, Y., Gu, Y., Yan, B., Li, Q."Efficacy, safety and influencing factors of intra‑calf muscular injection of bone marrow mononuclear cells in the treatment of type 2 diabetes mellitus‑induced lower extremity vascular disease". Experimental and Therapeutic Medicine 14, no. 5 (2017): 5177-5185. https://doi.org/10.3892/etm.2017.5193
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