Open Access

CHAF1A, the largest subunit of the chromatin assembly factor 1 complex, regulates the growth of H1299 human non‑small cell lung cancer cells by inducing G0/G1 cell cycle arrest

  • Authors:
    • Tanzhen Liu
    • Jingjing Wei
    • Chao Jiang
    • Chen Wang
    • Xiaoqin Zhang
    • Yan Du
    • Jianqiang Li
    • Hui Zhao
  • View Affiliations

  • Published online on: September 25, 2017     https://doi.org/10.3892/etm.2017.5201
  • Pages: 4681-4686
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Chromatin assembly factor 1 subunit A (CHAF1A) is the largest subunit of the chromatin assembly factor 1 (CAF‑1) complex that is implicated in the assembly of nucleosomes on newly synthesized DNA. The aim of the present study was to determine its expression and biological function in non‑small cell lung cancer (NSCLC). The current study examined the levels of CHAF1A expression in 22 samples of NSCLC and corresponding normal lung tissues. Subsequently, endogenous CHAF1A expression in H1299 NSCLC cells was knocked down via lentiviral delivery of CHAF1A‑targeting short hairpin RNA (shRNA), and cell proliferation, colony formation and cell cycle distribution were measured. The results demonstrated that levels of CHAF1A mRNA level were ~3‑fold greater in NSCLC samples compared with adjacent normal tissues (P<0.05). shRNA‑mediated silencing of CHAF1A significantly inhibited the proliferation and colony formation of H1299 cells, compared wirh the delivery of control shRNA (P<0.05). Furthermore, CHAF1A shRNA‑transduced cells exhibited a significant increase in the percentage of S‑phase cells and a significant decrease in the percentage of cells at the G0/G1 and G2/M phases, compared with control cells (P<0.05). Additionally, CHAF1A knockdown significantly decreased the expression of cyclin D1, cyclin‑dependent kinase 2 and S‑phase kinase‑associated protein 2, and increased the expression of p21 and p27. This indicates that CHAF1A is upregulated in NSCLC and that its silencing suppresses the proliferation and colony formation of NSCLC cells, potentially by inducing G0/G1 cell cycle arrest. CHAF1A may therefore represent a potential therapeutic target to treat NSCLC.
View Figures
View References

Related Articles

Journal Cover

November-2017
Volume 14 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Liu T, Wei J, Jiang C, Wang C, Zhang X, Du Y, Li J and Zhao H: CHAF1A, the largest subunit of the chromatin assembly factor 1 complex, regulates the growth of H1299 human non‑small cell lung cancer cells by inducing G0/G1 cell cycle arrest. Exp Ther Med 14: 4681-4686, 2017.
APA
Liu, T., Wei, J., Jiang, C., Wang, C., Zhang, X., Du, Y. ... Zhao, H. (2017). CHAF1A, the largest subunit of the chromatin assembly factor 1 complex, regulates the growth of H1299 human non‑small cell lung cancer cells by inducing G0/G1 cell cycle arrest. Experimental and Therapeutic Medicine, 14, 4681-4686. https://doi.org/10.3892/etm.2017.5201
MLA
Liu, T., Wei, J., Jiang, C., Wang, C., Zhang, X., Du, Y., Li, J., Zhao, H."CHAF1A, the largest subunit of the chromatin assembly factor 1 complex, regulates the growth of H1299 human non‑small cell lung cancer cells by inducing G0/G1 cell cycle arrest". Experimental and Therapeutic Medicine 14.5 (2017): 4681-4686.
Chicago
Liu, T., Wei, J., Jiang, C., Wang, C., Zhang, X., Du, Y., Li, J., Zhao, H."CHAF1A, the largest subunit of the chromatin assembly factor 1 complex, regulates the growth of H1299 human non‑small cell lung cancer cells by inducing G0/G1 cell cycle arrest". Experimental and Therapeutic Medicine 14, no. 5 (2017): 4681-4686. https://doi.org/10.3892/etm.2017.5201