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Correlation between RAGE gene promoter methylation and diabetic retinal inflammation

  • Authors:
    • Shifeng Kan
    • Jing Wu
    • Chengxi Sun
    • Jing Hao
    • Zhen Wu
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, Qilu Hospital, Shandong University, Jinan, Shangdong 250012, P.R. China, Department of Pharmacy, The Second Hospital of Shandong University, Jinan, Shangdong 250033, P.R. China, Department of Clinical Laboratory, The Second Hospital of Shandong University, Jinan, Shangdong 250033, P.R. China
    Copyright: © Kan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 242-246
    |
    Published online on: October 26, 2017
       https://doi.org/10.3892/etm.2017.5378
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Abstract

The methylation status of the receptor for advanced glycation end products (RAGE) gene promoter in peripheral blood mononuclear cells (PBMCs) of type 2 diabetic retinopathy (DR) patients was evaluated to investigate the correlation between RAGE gene promoter methylation and diabetic retinal inflammation. Eighty patients admitted and diagnosed as type 2 DR in Qilu Hospital, Shandong University during the period from October, 2013 to October, 2015 were enrolled in this study. They were the observation group and 40 healthy subjects were enrolled in the control group. PBMCs were collected from patients using density gradient centrifugation, and the methylation status of RAGE gene promoters was detected using methylation-specific PCP (MSP). Interleukin-1β (IL-1β), IL-6 and tumor necrosis factor-α (TNF-α) levels of in the serum were measured using enzyme-linked immunosorbent assay (ELISA). PBMCs in patients with positive RAGE gene promoter methylation were isolated and cultured and RAGE gene promoter methylation was inhibited using the demethylating agent, 5'-aza-2'-deoxycytidine (5-aza-dC). The methylation status of RAGE gene promoters in PBMCs was detected via MSP. IL-1β, IL-6 and TNF-α levels in the supernatant of PBMC culture solution were evaluated using ELISA. MSP results showed that there were 26 cases (32.50%) of RAGE gene promoter methylation in PBMCs in DR patients. RAGE gene promoters were methylated in all normal healthy subjects. IL-1β, IL-6 and TNF-α levels in serum for positive RAGE gene promoter methylation group were significantly lower than those in negative RAGE gene promoter methylation group (p<0.01). 5-aza-dC inhibited the RAGE gene promoter methylation of PBMCs in patients with positive RAGE gene promoter methylation. The inhibition of methylation in RAGE gene promoter increased the levels of IL-1β, IL-6 and TNF-α in supernatant of culture solution. In conclusion, RAGE gene promoter hypomethylation was detected in DR patients, indicating that RAGE gene promoter methylation could inhibit the diabetic retinal inflammation.
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Copy and paste a formatted citation
Spandidos Publications style
Kan S, Wu J, Sun C, Hao J and Wu Z: Correlation between RAGE gene promoter methylation and diabetic retinal inflammation. Exp Ther Med 15: 242-246, 2018.
APA
Kan, S., Wu, J., Sun, C., Hao, J., & Wu, Z. (2018). Correlation between RAGE gene promoter methylation and diabetic retinal inflammation. Experimental and Therapeutic Medicine, 15, 242-246. https://doi.org/10.3892/etm.2017.5378
MLA
Kan, S., Wu, J., Sun, C., Hao, J., Wu, Z."Correlation between RAGE gene promoter methylation and diabetic retinal inflammation". Experimental and Therapeutic Medicine 15.1 (2018): 242-246.
Chicago
Kan, S., Wu, J., Sun, C., Hao, J., Wu, Z."Correlation between RAGE gene promoter methylation and diabetic retinal inflammation". Experimental and Therapeutic Medicine 15, no. 1 (2018): 242-246. https://doi.org/10.3892/etm.2017.5378
Copy and paste a formatted citation
x
Spandidos Publications style
Kan S, Wu J, Sun C, Hao J and Wu Z: Correlation between RAGE gene promoter methylation and diabetic retinal inflammation. Exp Ther Med 15: 242-246, 2018.
APA
Kan, S., Wu, J., Sun, C., Hao, J., & Wu, Z. (2018). Correlation between RAGE gene promoter methylation and diabetic retinal inflammation. Experimental and Therapeutic Medicine, 15, 242-246. https://doi.org/10.3892/etm.2017.5378
MLA
Kan, S., Wu, J., Sun, C., Hao, J., Wu, Z."Correlation between RAGE gene promoter methylation and diabetic retinal inflammation". Experimental and Therapeutic Medicine 15.1 (2018): 242-246.
Chicago
Kan, S., Wu, J., Sun, C., Hao, J., Wu, Z."Correlation between RAGE gene promoter methylation and diabetic retinal inflammation". Experimental and Therapeutic Medicine 15, no. 1 (2018): 242-246. https://doi.org/10.3892/etm.2017.5378
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