Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
January-2018 Volume 15 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2018 Volume 15 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Influence of cartilage interstitial fluid on gene expression in cruciate ligament fibroblasts

  • Authors:
    • Anna Osiecka‑Iwan
    • Stanisław Moskalewski
    • Anna Kosowska
    • Anna Hyc
  • View Affiliations / Copyright

    Affiliations: Department of Histology and Embryology, Medical University of Warsaw, PL‑02004 Warsaw, Poland
  • Pages: 387-392
    |
    Published online on: October 27, 2017
       https://doi.org/10.3892/etm.2017.5384
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Loading of articular cartilage during motion squeezes the fluid from the cartilage, termed cartilage interstitial fluid (CIF), which was found to influence gene expression in synovial membrane cells. After crucial ligaments damage, these cells are exposed to synovial fluid containing factors released from articular cartilage; the aim of the present study was to establish the influence of CIF and factors present in CIF (CIF‑like cocktails) on crucial ligament fibroblasts. CIF was squeezed from articular‑epiphyseal cartilage complexes of newborn rats. Fibroblasts were obtained from crucial ligaments of adult rat knee joints. Cells were cultured in control medium, CIF and CIF‑like cocktails, and the expression of selected genes was evaluated using quantitative PCR. CIF stimulated the expression of HAS1, HAS2, aggrecan, lubricin, MMP3, TIMP3 and TGFβ1. Expression of collagen type I, versican, MMP2, TIMP2, TNF and IL1β was inhibited. The CIF‑like cocktail stimulated HAS1, HAS2, collagen type I, versican, aggrecan, lubricin, TIMP1, TGFβ1, IL1β, IL6 and inhibited of MMP3 and TNF expression. Both agents exerted similar effects on the expression of HAS2, aggrecan, lubricin, TGFβ1 and TNF. CIF contains inhibitory and stimulatory factors affecting gene expression in crucial ligament fibroblasts and some of them were not included in the CIF‑like cocktail. Due to the powerful influence of CIF on crucial ligament fibroblasts and the synovial membrane, further studies on its composition are needed. An improved CIF like‑cocktail could be applied in the treatment of various joint or tendon ailments.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

de Carvalho RT, Ramos LA, Novaretti JV, Ribeiro LM, Szeles PR, Ingham SJ and Abdalla RJ: Relationship between the middle genicular artery and the posterior structures of the knee: A cadaveric study. Orthop J Sports Med. 4:23259671166735792016. View Article : Google Scholar : PubMed/NCBI

2 

Duthon VB, Barea C, Abrassart S, Fasel JH, Fritschy DJ and Ménétrey J: Anatomy of the anterior cruciate ligament. Knee Surg Sports Traumatol Arthrosc. 14:204–213. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Andrish J and Holmes R: Effects of synovial fluid on fibroblasts in tissue culture. Clin Orthop Relat Res. 138:279–283. 1979.

4 

Murray MM, Spindler KP, Abreu E, Muller JA, Nedder A, Kelly M, Frino J, Zurakowski D, Valenza M, Snyder BD and Connolly SA: Collagen-platelet rich plasma hydrogel enhances primary repair of the porcine anterior cruciate ligament. J Orthop Res. 25:81–91. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Murray MM and Fleming BC: Use of a bioactive scaffold to stimulate anterior cruciate ligament healing also minimizes posttraumatic osteoarthritis after surgery. Am J Sports Med. 41:1762–1770. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Cheng M, Wang H, Yoshida R and Murray MM: Platelets and plasma proteins are both required to stimulate collagen gene expression by anterior cruciate ligament cells in three-dimensional culture. Tissue Eng Part A. 16:1479–1489. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Lee J, Green MH and Amiel DJ: Synergistic effect of growth factors on cell outgrowth from explants of rabbit anterior cruciate and medial collateral ligaments. J Orthop Res. 13:435–441. 1995. View Article : Google Scholar : PubMed/NCBI

8 

Spindler KP, Imro AK, Mayes CE and Davidson JM: Patellar tendon and anterior cruciate ligament have different mitogenic responses to platelet-derived growth factor and transforming growth factor beta. J Orthop Res. 14:542–546. 1996. View Article : Google Scholar : PubMed/NCBI

9 

Wang Y, Tang Z, Xue R, Singh GK, Lv Y, Shi K, Cai K, Deng L and Yang L: TGF-β1 promoted MMP-2 mediated wound healing of anterior cruciate ligament fibroblasts through NF-κB. Connect Tissue Res. 52:218–225. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Xie J, Jiang J, Zhang Y, Xu C, Yin L, Wang C, Chen PC and Sung KL: Up-regulation expressions of lysyl oxidase family in anterior cruciate ligament and medial collateral ligament fibroblasts induced by transforming growth factor-beta 1. Int Orthop. 36:207–213. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Xie J, Jiang J, Huang W, Zhang Y, Xu C, Wang C, Yin L, Chen PC and Sung KL: TNF-α induced down-regulation of lysyl oxidase family in anterior cruciate ligament and medial collateral ligament fibroblasts. Knee. 21:47–53. 2014. View Article : Google Scholar : PubMed/NCBI

12 

McCutchen CW: Sponge-hydrostatic and weeping bearings. Nature. 184:1284–1285. 1959. View Article : Google Scholar : PubMed/NCBI

13 

Caligaris M and Ateshian GA: Effects of sustained interstitial fluid pressurization under migrating contact area and boundary lubrication by synovial fluid, on cartilage friction. Osteoarthritis Cartilage. 16:1220–1227. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Hyc A, Moskalewski S and Osiecka-Iwan A: Influence of cartilage interstitial fluid on the mRNA levels of matrix proteins, cytokines, metalloproteases and their inhibitors in synovial membrane. Int J Mol Med. 38:937–942. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Hyc A, Moskalewski S, Stankevic D and Osiecka-Iwan A: Influence of cartilage interstitial fluid on gene expression in dermal fibroblasts. Folia Biologica (Kraków). 65:27–32. 2017.

16 

Osiecka-Iwan A, Moskalewski S and Hyc A: Influence of IGF1, TGFb1, bFGF and G-CSF/M-CSF on the mRNA levels of selected matrix proteins, cytokines, metalloproteinase 3 and TIMP1 in rat synovial membrane cells. Folia Histochem Cytobiol. 54:159–165. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Stuhlmeier KM and Pollaschek C: Differential effect of transforming growth factor beta (TGF-beta) on the genes encoding hyaluronan synthases and utilization of the p38 MAPK pathway in TGF-beta-induced hyaluronan synthase 1 activation. J Biol Chem. 279:8753–8760. 2004. View Article : Google Scholar : PubMed/NCBI

19 

Kabiri A, Esfandiari E, Esmaeili A, Hashemibeni B, Pourazar A and Mardani M: Platelet-rich plasma application in chondrogenesis. Adv Biomed Res. 3:1382014. View Article : Google Scholar : PubMed/NCBI

20 

Young K, Samiric T, Feller J and Cook J: Extracellular matrix content of ruptured anterior cruciate ligament tissue. Knee. 18:242–246. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Zhang D, Cheriyan T, Martin SD, Gomoll AH, Schmid TM and Spector M: Lubricin distribution in the torn human anterior cruciate ligament and meniscus. J Orthop Res. 29:1916–1922. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Milz S, Aktas T, Putz R and Benjamin M: Expression of extracellular matrix molecules typical of articular cartilage in the human scapholunate interosseous ligament. J Anat. 208:671–679. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Higuchi H, Shirakura K, Kimura M, Terauchi M, Shinozaki T, Watanabe H and Takagishi K: Changes in biochemical parameters after anterior cruciate ligament injury. Int Orthop. 30:43–47. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Ireland D, Harrall R, Curry V, Holloway G, Hackney R, Hazleman B and Riley G: Multiple changes in gene expression in chronic human Achilles tendinopathy. Matrix Biol. 20:159–169. 2001. View Article : Google Scholar : PubMed/NCBI

25 

Skutek M, van Griensven M, Zeichen J, Brauer N and Bosch U: Cyclic mechanical stretching modulates secretion pattern of growth factors in human tendon fibroblasts. Eur J Appl Physiol. 86:48–52. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Melas IN, Chairakaki AD, Chatzopoulou EI, Messinis DE, Katopodi T, Pliaka V, Samara S, Mitsos A, Dailiana Z, Kollia P and Alexopoulos LG: Modeling of signaling pathways in chondrocytes based on phosphoproteomic and cytokine release data. Osteoarthritis Cartilage. 22:509–518. 2014. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Osiecka‑Iwan A, Moskalewski S, Kosowska A and Hyc A: Influence of cartilage interstitial fluid on gene expression in cruciate ligament fibroblasts. Exp Ther Med 15: 387-392, 2018.
APA
Osiecka‑Iwan, A., Moskalewski, S., Kosowska, A., & Hyc, A. (2018). Influence of cartilage interstitial fluid on gene expression in cruciate ligament fibroblasts. Experimental and Therapeutic Medicine, 15, 387-392. https://doi.org/10.3892/etm.2017.5384
MLA
Osiecka‑Iwan, A., Moskalewski, S., Kosowska, A., Hyc, A."Influence of cartilage interstitial fluid on gene expression in cruciate ligament fibroblasts". Experimental and Therapeutic Medicine 15.1 (2018): 387-392.
Chicago
Osiecka‑Iwan, A., Moskalewski, S., Kosowska, A., Hyc, A."Influence of cartilage interstitial fluid on gene expression in cruciate ligament fibroblasts". Experimental and Therapeutic Medicine 15, no. 1 (2018): 387-392. https://doi.org/10.3892/etm.2017.5384
Copy and paste a formatted citation
x
Spandidos Publications style
Osiecka‑Iwan A, Moskalewski S, Kosowska A and Hyc A: Influence of cartilage interstitial fluid on gene expression in cruciate ligament fibroblasts. Exp Ther Med 15: 387-392, 2018.
APA
Osiecka‑Iwan, A., Moskalewski, S., Kosowska, A., & Hyc, A. (2018). Influence of cartilage interstitial fluid on gene expression in cruciate ligament fibroblasts. Experimental and Therapeutic Medicine, 15, 387-392. https://doi.org/10.3892/etm.2017.5384
MLA
Osiecka‑Iwan, A., Moskalewski, S., Kosowska, A., Hyc, A."Influence of cartilage interstitial fluid on gene expression in cruciate ligament fibroblasts". Experimental and Therapeutic Medicine 15.1 (2018): 387-392.
Chicago
Osiecka‑Iwan, A., Moskalewski, S., Kosowska, A., Hyc, A."Influence of cartilage interstitial fluid on gene expression in cruciate ligament fibroblasts". Experimental and Therapeutic Medicine 15, no. 1 (2018): 387-392. https://doi.org/10.3892/etm.2017.5384
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team