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Article

miR-4262 regulates chondrocyte viability, apoptosis, autophagy by targeting SIRT1 and activating PI3K/AKT/mTOR signaling pathway in rats with osteoarthritis

  • Authors:
    • Wencai Sun
    • Yintai Li
    • Suizhuan Wei
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, The Third Hospital Affiliated to Qiqihar Medical College, Qiqihar, Heilongjiang 161006, P.R. China, Department of Rehabilitation, Baoji Traditional Chinese Medicine Hospital, Baoji, Shaanxi 721000, P.R. China, Department of Orthopaedics, Baoji Traditional Chinese Medicine Hospital, Baoji, Shaanxi 721000, P.R. China
  • Pages: 1119-1128
    |
    Published online on: November 6, 2017
       https://doi.org/10.3892/etm.2017.5444
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Abstract

The present study aimed to investigate the effect and underlying mechanism of microRNA (miR)‑4262 in the development of osteoarthritis (OA) in rats. Primary chondrocytes were separated from Sprague‑Dawley rats and then treated with tumor necrosis factor‑α (TNF‑α). The level of miR‑4262 was detected in TNF‑α‑treated chondrocytes, and then the miR‑4262 or its target gene sirtuin type 1 (SIRT1) level was overexpressed, or knocked down. Furthermore, cell viability, cell apoptosis, cell autophagy and matrix synthesis, as well as the expressions of proteins associated with the phosphoinositide 3‑kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signaling pathway were detected. miR‑4262 was significantly overexpressed in TNF‑α‑treated chondrocytes compared with untreated cells (P<0.05). TNF‑α treatment or miR‑4262 overexpression significantly decreased cell viability, autophagy‑related proteins levels and matrix synthesis‑related proteins levels, as well as increased the apoptotic rate in chondrocytes (P<0.05). Overexpression of SIRT1 significantly increased cell viability, autophagy‑related proteins levels and matrix synthesis‑related proteins levels, as well as decreased the apoptotic rate in TNF‑α‑treated chondrocytes (P<0.05). In addition, the effects of miR‑4262 on cell viability, cell apoptosis, cell autophagy and matrix synthesis were inhibited by SIRT1 (P<0.05). Furthermore, upregulated miR‑4262 remarkably increased the expressions of phosphorylated (p)‑PI3K, p‑AKT and p‑mTOR (P<0.05) in TNF‑α treated chondrocytes. The present study revealed that the upregulation of miR‑4262 may promote the occurrence and development of OA in rats by regulating cell viability, cell apoptosis, cell autophagy, and matrix synthesis. Furthermore, these roles of miR‑4262 may be associated with PI3K/AKT/mTOR signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Sun W, Li Y and Wei S: miR-4262 regulates chondrocyte viability, apoptosis, autophagy by targeting SIRT1 and activating PI3K/AKT/mTOR signaling pathway in rats with osteoarthritis. Exp Ther Med 15: 1119-1128, 2018.
APA
Sun, W., Li, Y., & Wei, S. (2018). miR-4262 regulates chondrocyte viability, apoptosis, autophagy by targeting SIRT1 and activating PI3K/AKT/mTOR signaling pathway in rats with osteoarthritis. Experimental and Therapeutic Medicine, 15, 1119-1128. https://doi.org/10.3892/etm.2017.5444
MLA
Sun, W., Li, Y., Wei, S."miR-4262 regulates chondrocyte viability, apoptosis, autophagy by targeting SIRT1 and activating PI3K/AKT/mTOR signaling pathway in rats with osteoarthritis". Experimental and Therapeutic Medicine 15.1 (2018): 1119-1128.
Chicago
Sun, W., Li, Y., Wei, S."miR-4262 regulates chondrocyte viability, apoptosis, autophagy by targeting SIRT1 and activating PI3K/AKT/mTOR signaling pathway in rats with osteoarthritis". Experimental and Therapeutic Medicine 15, no. 1 (2018): 1119-1128. https://doi.org/10.3892/etm.2017.5444
Copy and paste a formatted citation
x
Spandidos Publications style
Sun W, Li Y and Wei S: miR-4262 regulates chondrocyte viability, apoptosis, autophagy by targeting SIRT1 and activating PI3K/AKT/mTOR signaling pathway in rats with osteoarthritis. Exp Ther Med 15: 1119-1128, 2018.
APA
Sun, W., Li, Y., & Wei, S. (2018). miR-4262 regulates chondrocyte viability, apoptosis, autophagy by targeting SIRT1 and activating PI3K/AKT/mTOR signaling pathway in rats with osteoarthritis. Experimental and Therapeutic Medicine, 15, 1119-1128. https://doi.org/10.3892/etm.2017.5444
MLA
Sun, W., Li, Y., Wei, S."miR-4262 regulates chondrocyte viability, apoptosis, autophagy by targeting SIRT1 and activating PI3K/AKT/mTOR signaling pathway in rats with osteoarthritis". Experimental and Therapeutic Medicine 15.1 (2018): 1119-1128.
Chicago
Sun, W., Li, Y., Wei, S."miR-4262 regulates chondrocyte viability, apoptosis, autophagy by targeting SIRT1 and activating PI3K/AKT/mTOR signaling pathway in rats with osteoarthritis". Experimental and Therapeutic Medicine 15, no. 1 (2018): 1119-1128. https://doi.org/10.3892/etm.2017.5444
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