Evaluation of the inactivation effect of riboflavin photochemical method on duck hepatitis B virus

Zhou,Zi-Yu; Bi,Xing-Xiu

doi:10.3892/etm.2017.5507

January-2018 Volume 15 Issue 1

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January-2018 Volume 15 Issue 1

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Article Open Access

Evaluation of the inactivation effect of riboflavin photochemical method on duck hepatitis B virus

Authors:
- Zi-Yu Zhou
- Xing-Xiu Bi
View Affiliations / Copyright

Affiliations: Xuzhou Red Cross Blood Station, Xuzhou, Jiangsu 221006, P.R. China

Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 751-754
|
Published online on: November 13, 2017

https://doi.org/10.3892/etm.2017.5507
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Abstract

Hepatitis B virus (HBV) is a common and widespread infection that poses a serious threat among carriers for the development of life-threatening liver diseases. The aim of the present study was to evaluate the effectiveness of the riboflavin photochemical method in inactivating duck hepatitis B virus (DHBV) in plasma via an animal model. Forty ducks were selected and randomly divided into the experimental (n=10), the virus control (n=10), the visible light control (n=10) and the plasma control group (n=10). Ducks in the experimental group were injected with plasma inactivated by the riboflavin photochemical method; in the virus control group were injected with plasma without inactivation treatment; in the visible light control group were injected with plasma irradiated by visible light; and in the plasma control group were injected with normal plasma. The serum of the ducks in each group was taken at different time points to detect DHBV-DNA levels via FQ-PCR and duck hepatitis B surface antigen (DHBsAg) via ELISA. DHBV-DNA in the experimental group was decreased gradually over time until it disappeared and there was a significant difference in DHBsAg between the experimental and control groups (P<0.05). In conclusion, the results showed that the riboflavin photochemical method is effective in the inactivation of viruses in plasma, which has relevance for preventive strategies against transfusion-derived infections.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Evaluation of the inactivation effect of riboflavin photochemical method on duck hepatitis B virus

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