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Immunosuppressive effects of hydroxychloroquine and artemisinin combination therapy via the nuclear factor‑κB signaling pathway in lupus nephritis mice

  • Authors:
    • Ning Liang
    • Yanchun Zhong
    • Jie Zhou
    • Bihao Liu
    • Ruirui Lu
    • Yezhi Guan
    • Qi Wang
    • Chunlin Liang
    • Yu He
    • Yuan Zhou
    • Jianping Song
    • Jiuyao Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, College of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, P.R. China, Science and Technology Industrial Park, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510445, P.R. China, Artepharm Co., Ltd., Guangzhou, Guangdong 510032, P.R. China
    Copyright: © Liang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2436-2442
    |
    Published online on: January 5, 2018
       https://doi.org/10.3892/etm.2018.5708
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Abstract

Lupus nephritis (LN) is one of the most common and severe manifestations of systemic lupus erythematosus, leading to permanent renal damage and chronic kidney disease. Hydroxychloroquine (HCQ) serves a protective role against lupus-associated clinical manifestations and medical complications; however, it results in numerous adverse reactions, limiting its long‑term use. The aim of the present study was to investigate the combined effect of HCQ and artemisinin (ART) on LN, and to elucidate the underlying mechanisms. An in vivo LN mouse model was prepared, and the animals were administered prednisone (PDS; serving as a positive control), high‑dose HCQ (H‑HCQ) or low‑dose HCQ combined with ART (L‑HCQ + ART) once daily for 8 weeks. The body weight, serum biochemical parameters, immune and inflammatory indicators, renal and spleen histological alterations, and mRNA expression levels of Kruppel‑like factor 15 (KLF15) and nuclear factor‑κB (NF‑κB) were analyzed. It was observed that L‑HCQ + ART and H‑HCQ ameliorated the LN‑induced body weight decrease, and significantly decreased the levels of anti‑double stranded DNA, antinuclear antibodies, immunoglobulin G, interferon γ, tumor necrosis factor‑α and transforming growth factor‑β1, as well as improved the kidney and spleen pathology, when compared with the model group. In addition, L‑HCQ + ART and H‑HCQ treatments induced KLF15 upregulation and NF‑κB downregulation. These results indicated that treatment with L‑HCQ + ART exerted renoprotective effects by regulating the expression levels of cytokines, KLF15 and NF‑κB. This combination treatment may have a similar immunosuppressive effect as PDS and H‑HCQ, and may be a promising alternative for LN treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Liang N, Zhong Y, Zhou J, Liu B, Lu R, Guan Y, Wang Q, Liang C, He Y, Zhou Y, Zhou Y, et al: Immunosuppressive effects of hydroxychloroquine and artemisinin combination therapy via the nuclear factor‑κB signaling pathway in lupus nephritis mice. Exp Ther Med 15: 2436-2442, 2018.
APA
Liang, N., Zhong, Y., Zhou, J., Liu, B., Lu, R., Guan, Y. ... Zhou, J. (2018). Immunosuppressive effects of hydroxychloroquine and artemisinin combination therapy via the nuclear factor‑κB signaling pathway in lupus nephritis mice. Experimental and Therapeutic Medicine, 15, 2436-2442. https://doi.org/10.3892/etm.2018.5708
MLA
Liang, N., Zhong, Y., Zhou, J., Liu, B., Lu, R., Guan, Y., Wang, Q., Liang, C., He, Y., Zhou, Y., Song, J., Zhou, J."Immunosuppressive effects of hydroxychloroquine and artemisinin combination therapy via the nuclear factor‑κB signaling pathway in lupus nephritis mice". Experimental and Therapeutic Medicine 15.3 (2018): 2436-2442.
Chicago
Liang, N., Zhong, Y., Zhou, J., Liu, B., Lu, R., Guan, Y., Wang, Q., Liang, C., He, Y., Zhou, Y., Song, J., Zhou, J."Immunosuppressive effects of hydroxychloroquine and artemisinin combination therapy via the nuclear factor‑κB signaling pathway in lupus nephritis mice". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2436-2442. https://doi.org/10.3892/etm.2018.5708
Copy and paste a formatted citation
x
Spandidos Publications style
Liang N, Zhong Y, Zhou J, Liu B, Lu R, Guan Y, Wang Q, Liang C, He Y, Zhou Y, Zhou Y, et al: Immunosuppressive effects of hydroxychloroquine and artemisinin combination therapy via the nuclear factor‑κB signaling pathway in lupus nephritis mice. Exp Ther Med 15: 2436-2442, 2018.
APA
Liang, N., Zhong, Y., Zhou, J., Liu, B., Lu, R., Guan, Y. ... Zhou, J. (2018). Immunosuppressive effects of hydroxychloroquine and artemisinin combination therapy via the nuclear factor‑κB signaling pathway in lupus nephritis mice. Experimental and Therapeutic Medicine, 15, 2436-2442. https://doi.org/10.3892/etm.2018.5708
MLA
Liang, N., Zhong, Y., Zhou, J., Liu, B., Lu, R., Guan, Y., Wang, Q., Liang, C., He, Y., Zhou, Y., Song, J., Zhou, J."Immunosuppressive effects of hydroxychloroquine and artemisinin combination therapy via the nuclear factor‑κB signaling pathway in lupus nephritis mice". Experimental and Therapeutic Medicine 15.3 (2018): 2436-2442.
Chicago
Liang, N., Zhong, Y., Zhou, J., Liu, B., Lu, R., Guan, Y., Wang, Q., Liang, C., He, Y., Zhou, Y., Song, J., Zhou, J."Immunosuppressive effects of hydroxychloroquine and artemisinin combination therapy via the nuclear factor‑κB signaling pathway in lupus nephritis mice". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2436-2442. https://doi.org/10.3892/etm.2018.5708
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