Correlation between PPAR-α methylation level in peripheral blood and atherosclerosis of NAFLD patients with DM

Ju,Jianghua; Huang,Qingxian; Sun,Jing; Zhao,Xiaoyun; Guo,Xiuli; Jin,Yongcheng; Ma,Wenjie; Wang,Wenhui

doi:10.3892/etm.2018.5730

March-2018 Volume 15 Issue 3

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March-2018 Volume 15 Issue 3

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Article Open Access

Correlation between PPAR-α methylation level in peripheral blood and atherosclerosis of NAFLD patients with DM

Authors:
- Jianghua Ju
- Qingxian Huang
- Jing Sun
- Xiaoyun Zhao
- Xiuli Guo
- Yongcheng Jin
- Wenjie Ma
- Wenhui Wang
View Affiliations / Copyright

Affiliations: Department of Endocrinology, Qilu Hospital of Shandong University, Qingdao, Shandong 266035, P.R. China

Copyright: © Ju et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 2727-2730
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Published online on: January 10, 2018

https://doi.org/10.3892/etm.2018.5730
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Abstract

We investigated the correlation between the methylation levels of peroxisome proliferator-activated receptor-α (PPAR-α) in the peripheral blood and atherosclerosis in patients with nonalcoholic fatty liver disease (NAFLD) with diabetes mellitus (DM). A total of 50 normal subjects (group N) and 50 NAFLD patients with DM (group M) were selected at Qilu Hospital of Shandong University. The levels of total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) in groups N and M were detected. The mRNA and protein expressions of PPAR-α in groups N and M were detected using reverse transcription polymerase chain reaction (PCR) and immunofluorescence. The differences in expression of PPAR-α in group N and M and the correlation between PPAR-α methylation level and atherosclerosis were analyzed using SPSS17.0 statistical software. TC, TG, HDL and LDL in groups N and M were significantly different (P<0.01). Hematoxylin and eosin staining showed that the histopathological damage was severe in group M. PCR and immunofluorescence showed that PPAR-α was significantly higher in N than in group M (P<0.01). The abnormal expression of PPAR-α is closely related to atherosclerosis, indicating that the correlation between PPAR-α methylation levels in peripheral blood and atherosclerosis of NAFLD patients with DM can provide a new direction of diagnosis and treatment.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Correlation between PPAR-α methylation level in peripheral blood and atherosclerosis of NAFLD patients with DM

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