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Print ISSN: 1792-0981 Online ISSN: 1792-1015
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March-2018 Volume 15 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Article

Assessment of recombinant plasmid expressing fusion antigen Ag85B-Rv3425 in management of acute tuberculosis infection in mice

  • Authors:
    • Huichang Huang
    • Feifei Wang
    • Enzhuo Yang
    • Honghai Wang
    • Peng Gao
    • Hongbo Shen
  • View Affiliations / Copyright

    Affiliations: Unit of Anti‑Tuberculosis Immunity, CAS Key Laboratory of Molecular Virology and Immunology, Institute Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200031, P.R. China, Department of Medical Microbiology and Parasitology, Shanghai Medical College, Fudan University, Shanghai 200032, P.R. China, State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200433, P.R. China, Department of Urology, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China
  • Pages: 3034-3039
    |
    Published online on: January 23, 2018
       https://doi.org/10.3892/etm.2018.5785
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Abstract

The emergence of drug-resistant tuberculosis (TB) and HIV-TB co‑infection fuels an urgent need to develop novel therapeutic approaches, including therapeutic vaccines. Therapeutic vaccines have been proven to be a good strategy by inducing antigen specific immune responses against TB infection. In the present study, a recombinant plasmid based on lentiviral vector expressing fusion antigen Ag85B‑Rv3425 (A3), and was constructed the immunogenicity and treatment effects in TB mice were assessed. The results showed that A3 delivered by the plasmid could be expressed appropriately in vivo and induced higher production of tumor necrosis factor‑α and interleukin‑2 compared with A3 recombinant protein in mice. Moreover, the recombinant plasmid expressing A3 confered resistance to acute TB infection in mice, characterized by a reduction in the bacterial load in the lungs and spleen, as well as attenuated TB lesions in lung tissues. These results implicated that the recombinant plasmid based on lentiviral vector expressing A3 is a potent and promising therapeutic agent to treat acute TB infection.
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Copy and paste a formatted citation
Spandidos Publications style
Huang H, Wang F, Yang E, Wang H, Gao P and Shen H: Assessment of recombinant plasmid expressing fusion antigen Ag85B-Rv3425 in management of acute tuberculosis infection in mice. Exp Ther Med 15: 3034-3039, 2018.
APA
Huang, H., Wang, F., Yang, E., Wang, H., Gao, P., & Shen, H. (2018). Assessment of recombinant plasmid expressing fusion antigen Ag85B-Rv3425 in management of acute tuberculosis infection in mice. Experimental and Therapeutic Medicine, 15, 3034-3039. https://doi.org/10.3892/etm.2018.5785
MLA
Huang, H., Wang, F., Yang, E., Wang, H., Gao, P., Shen, H."Assessment of recombinant plasmid expressing fusion antigen Ag85B-Rv3425 in management of acute tuberculosis infection in mice". Experimental and Therapeutic Medicine 15.3 (2018): 3034-3039.
Chicago
Huang, H., Wang, F., Yang, E., Wang, H., Gao, P., Shen, H."Assessment of recombinant plasmid expressing fusion antigen Ag85B-Rv3425 in management of acute tuberculosis infection in mice". Experimental and Therapeutic Medicine 15, no. 3 (2018): 3034-3039. https://doi.org/10.3892/etm.2018.5785
Copy and paste a formatted citation
x
Spandidos Publications style
Huang H, Wang F, Yang E, Wang H, Gao P and Shen H: Assessment of recombinant plasmid expressing fusion antigen Ag85B-Rv3425 in management of acute tuberculosis infection in mice. Exp Ther Med 15: 3034-3039, 2018.
APA
Huang, H., Wang, F., Yang, E., Wang, H., Gao, P., & Shen, H. (2018). Assessment of recombinant plasmid expressing fusion antigen Ag85B-Rv3425 in management of acute tuberculosis infection in mice. Experimental and Therapeutic Medicine, 15, 3034-3039. https://doi.org/10.3892/etm.2018.5785
MLA
Huang, H., Wang, F., Yang, E., Wang, H., Gao, P., Shen, H."Assessment of recombinant plasmid expressing fusion antigen Ag85B-Rv3425 in management of acute tuberculosis infection in mice". Experimental and Therapeutic Medicine 15.3 (2018): 3034-3039.
Chicago
Huang, H., Wang, F., Yang, E., Wang, H., Gao, P., Shen, H."Assessment of recombinant plasmid expressing fusion antigen Ag85B-Rv3425 in management of acute tuberculosis infection in mice". Experimental and Therapeutic Medicine 15, no. 3 (2018): 3034-3039. https://doi.org/10.3892/etm.2018.5785
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