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Article

High calcium diet alleviates 5/6 nephrectomy-induced bone deteriorations of lumbar vertebrae in mice

  • Authors:
    • Hai‑Tao Hou
    • Ya‑Nan Wang
    • Shi‑Ze Shao
    • Song Fu
    • Xiang‑Peng Huang
    • Xiao‑Hui Wang
  • View Affiliations / Copyright

    Affiliations: Department of Spinal and Spinal Cord Surgery, Shandong Wendeng Orthopedic Hospital, Wendeng, Shandong 264400, P.R. China
  • Pages: 3483-3488
    |
    Published online on: February 14, 2018
       https://doi.org/10.3892/etm.2018.5866
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Abstract

Dietary calcium (Ca) supplementation has beneficial effects on bone health. However, it is not clear whether a high calcium diet (HCD) following 5/6 nephrectomy (5/6 Nx) is beneficial to bone health. The aim of the present study was to examine the effects of an HCD on bone metabolism using a chronic kidney disease (CKD) mouse model. Male C57BL/6J mice were divided into three groups: Sham group, 5/6 Nx group and 5/6 Nx + HCD group. Mice were sacrificed 12 weeks post‑surgery. Calcium (Ca) and creatinine (Cr) were measured using standard colorimetric methods and picric acid methods, respectively. Bone metabolism‑associated markers, FGF‑23, PTH, ALP‑b and TRAP‑5b were measured using ELISA kits. Lumbar vertebrae histomorphological analysis was performed using hematoxylin and eosin staining. The expression of osteoprotegerin (OPG) and receptor activator of nuclear factor κB ligand (RANKL) mRNA was detected using reverse transcription‑quantitative polymerase chain reaction. Impaired renal function and histopathological damage was indicated in 5/6 Nx mice. However, HCD had no significant effects on these changes in 5/6 Nx mice. Notably, mineral metabolism disorder and histopathological damage to lumbar vertebrae were markedly improved in HCD‑treated 5/6 Nx mice. Compared with 5/6 Nx mice, HCD supplementation significantly elevated the ratio of OPG/RANKL and inhibited RANKL mRNA expression in lumbar vertebrae. To conclude, the present findings indicated that increased Ca intake is effective in increasing bone mineral content of the lumbar vertebrae in 5/6 Nx mice. These results may provide a basis for the clinical use of dietary Ca supplementation as a therapeutic approach to treat CKD‑induced disturbance of mineral metabolism and bone loss.
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Copy and paste a formatted citation
Spandidos Publications style
Hou HT, Wang YN, Shao SZ, Fu S, Huang XP and Wang XH: High calcium diet alleviates 5/6 nephrectomy-induced bone deteriorations of lumbar vertebrae in mice. Exp Ther Med 15: 3483-3488, 2018.
APA
Hou, H., Wang, Y., Shao, S., Fu, S., Huang, X., & Wang, X. (2018). High calcium diet alleviates 5/6 nephrectomy-induced bone deteriorations of lumbar vertebrae in mice. Experimental and Therapeutic Medicine, 15, 3483-3488. https://doi.org/10.3892/etm.2018.5866
MLA
Hou, H., Wang, Y., Shao, S., Fu, S., Huang, X., Wang, X."High calcium diet alleviates 5/6 nephrectomy-induced bone deteriorations of lumbar vertebrae in mice". Experimental and Therapeutic Medicine 15.4 (2018): 3483-3488.
Chicago
Hou, H., Wang, Y., Shao, S., Fu, S., Huang, X., Wang, X."High calcium diet alleviates 5/6 nephrectomy-induced bone deteriorations of lumbar vertebrae in mice". Experimental and Therapeutic Medicine 15, no. 4 (2018): 3483-3488. https://doi.org/10.3892/etm.2018.5866
Copy and paste a formatted citation
x
Spandidos Publications style
Hou HT, Wang YN, Shao SZ, Fu S, Huang XP and Wang XH: High calcium diet alleviates 5/6 nephrectomy-induced bone deteriorations of lumbar vertebrae in mice. Exp Ther Med 15: 3483-3488, 2018.
APA
Hou, H., Wang, Y., Shao, S., Fu, S., Huang, X., & Wang, X. (2018). High calcium diet alleviates 5/6 nephrectomy-induced bone deteriorations of lumbar vertebrae in mice. Experimental and Therapeutic Medicine, 15, 3483-3488. https://doi.org/10.3892/etm.2018.5866
MLA
Hou, H., Wang, Y., Shao, S., Fu, S., Huang, X., Wang, X."High calcium diet alleviates 5/6 nephrectomy-induced bone deteriorations of lumbar vertebrae in mice". Experimental and Therapeutic Medicine 15.4 (2018): 3483-3488.
Chicago
Hou, H., Wang, Y., Shao, S., Fu, S., Huang, X., Wang, X."High calcium diet alleviates 5/6 nephrectomy-induced bone deteriorations of lumbar vertebrae in mice". Experimental and Therapeutic Medicine 15, no. 4 (2018): 3483-3488. https://doi.org/10.3892/etm.2018.5866
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