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Article Open Access

MicroRNA-215 suppresses the proliferation, migration and invasion of non-small cell lung carcinoma cells through the downregulation of matrix metalloproteinase-16 expression

  • Authors:
    • Yuanshan Yao
    • Haibo Shen
    • Yinjie Zhou
    • Zhenhua Yang
    • Tianjun Hu
  • View Affiliations / Copyright

    Affiliations: Department of Chest Surgery, Ningbo No. 2 Hospital, Ningbo, Zhejiang 315000, P.R. China
    Copyright: © Yao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3239-3246
    |
    Published online on: February 14, 2018
       https://doi.org/10.3892/etm.2018.5869
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Abstract

The present study investigated the expression of microRNA (miR)‑215 in non‑small cell lung carcinoma (NSCLC) at tissue and cellular levels, as well as its biological functions and mechanism of action. A total of 56 patients with NSCLC were included in the present study. NSCLC tissues and tumor‑adjacent normal tissues were resected and collected. Reverse transcription‑quantitative polymerase chain reaction was used to measure the expression of miR‑215. Following transfection with miR‑215 mimics, A549 cell proliferation, migration and invasion were determined using a Cell Counting Kit‑8 and Transwell assay. Western blotting was employed to measure the expression of matrix metalloproteinase (MMP)‑16 protein. A dual‑luciferase reporter assay was conducted to determine the existence of a direct interaction between miR‑215 and the MMP‑16 gene. Reduced expression of miR‑215 in NSCLC was closely associated with lymphatic metastasis and TNM staging. Overexpression of miR‑215 inhibited the proliferation of A549 cells in vitro. Upregulated expression of miR‑215 inhibited the migration and invasion of A549 cells in vitro. miR‑215 exerted its biological functions possibly by regulating the expression of MMP‑16. Elevated expression of MMP‑16 promoted the proliferation, migration and invasion of A549 cells. miR‑215 regulated the proliferation, migration and invasion of A549 cells by binding with the seed 3'‑untranslated region of MMP‑16 mRNA. The present study demonstrates that reduced expression of miR‑215 in NSCLC is negatively associated with lymphatic metastasis and TNM staging. In addition, miR‑215 acts as a tumor suppressor gene by inhibiting the proliferation, migration and invasion of NSCLC cells via the downregulation of MMP‑16 expression.
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1 

Kaira K, Tomizawa Y, Imai H, Sakurai R, Matsuura M, Yoshii A, Ochiai M, Kotake M, Ebara T, Saitoh JI, et al: Phase I study of nab-paclitaxel plus carboplatin and concurrent thoracic radiotherapy in patients with locally advanced non-small cell lung cancer. Cancer Chemother Pharmacol. 79:165–171. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Dong Z, Zhao L, Lu S, Xiong J and Geng Z: Overexpression of TSPAN8 promotes tumor cell viability and proliferation in nonsmall cell lung cancer. Cancer Biother Radiopharm. 31:353–359. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Presley CJ, Soulos PR, Tinetti M, Montori VM, Yu JB and Gross CP: Treatment burden of medicare beneficiaries with stage I non-small-cell lung cancer. J Oncol Pract. 13:e98–e107. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Qiu ZL, Shen CT, Sun ZK, Wei WJ, Zhang XY, Song HJ and Luo QY: Circulating long non-coding rnas act as biomarkers for predicting 131I uptake and mortality in papillary thyroid cancer patients with lung metastases. Cell Physiol Biochem. 40:1377–1390. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Liu TC, Hsieh MJ, Wu WJ, Chou YE, Chiang WL, Yang SF, Su SC and Tsao TC: Association between survivin genetic polymorphisms and epidermal growth factor receptor mutation in non-small-cell lung cancer. Int J Med Sci. 13:929–935. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Ma L, Qiu B, Zhang J, Li QW, Wang B, Zhang XH, Qiang MY, Chen ZL, Guo SP and Liu H: Survival and prognostic factors of non-small cell lung cancer patients with postoperative locoregional recurrence treated with radical radiotherapy. Chin J Cancer. 36:932017. View Article : Google Scholar : PubMed/NCBI

7 

Wei WE, Mao NQ, Ning SF, Li JL, Liu HZ, Xie T, Zhong JH, Feng Y, Wei CH and Zhang LT: An analysis of EGFR mutations among 1506 cases of non-small cell lung cancer patients in Guangxi, China. PLoS One. 11:e01687952016. View Article : Google Scholar : PubMed/NCBI

8 

Ghafoor Q, Baijal S, Taniere P, O'Sullivan B, Evans M and Middleton G: Epidermal growth factor receptor (EGFR) kinase inhibitors and non-small cell lung cancer (NSCLC)-advances in molecular diagnostic techniques to facilitate targeted therapy. Pathol Oncol Res. Dec 21–2017.(Epub ahead of print). View Article : Google Scholar : PubMed/NCBI

9 

Wang T, Xu X, Xu Q, Ren J, Shen S, Fan C and Hou Y: miR-19a promotes colitis-associated colorectal cancer by regulating tumor necrosis factor alpha-induced protein 3-NF-κB feedback loops. Oncogene. 36:3240–3251. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Labourier E, Lloyd M, Andruss B, Adai A and Schwarzbach A: An miRNA assay for the classification of benign and neoplastic lesions in pancreatic fine-needle aspirates. J Clin Oncol. 29 4 Suppl:S1632011. View Article : Google Scholar

11 

Huang G, Pan J, Ye Z, Fang B, Cheng W and Cao Z: Overexpression of miR-216b sensitizes NSCLC cells to cisplatin-induced apoptosis by targeting c-Jun. Oncotarget. 8:104206–104215. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Pei K, Zhu JJ, Wang CE, Xie QL and Guo JY: MicroRNA-185-5p modulates chemosensitivity of human non-small cell lung cancer to cisplatin via targeting ABCC1. Eur Rev Med Pharmacol Sci. 20:4697–4704. 2016.PubMed/NCBI

13 

Sun CC, Li SJ, Zhang F, Zhang YD, Zuo ZY, Xi YY, Wang L and Li DJ: The novel miR-9600 suppresses tumor progression and promotes paclitaxel sensitivity in non-small-cell lung cancer through altering STAT3 expression. Mol Ther Nucleic Acids. 5:e3872016. View Article : Google Scholar : PubMed/NCBI

14 

Wang P, Deng Y and Fu X: MiR-509-5p suppresses the proliferation, migration, and invasion of non-small cell lung cancer by targeting YWHAG. Biochem Biophys Res Commun. 482:935–941. 2017. View Article : Google Scholar : PubMed/NCBI

15 

Wei Y, Sun J and Li X: MicroRNA-215 enhances invasion and migration by targeting retinoblastoma tumor suppressor gene 1 in high-grade glioma. Biotechnol Lett. 39:197–205. 2017. View Article : Google Scholar : PubMed/NCBI

16 

Li N, Zhang QY, Zou JL, Li ZW, Tian TT, Dong B, Liu XJ, Ge S, Zhu Y, Gao J and Shen L: miR-215 promotes malignant progression of gastric cancer by targeting RUNX1. Oncotarget. 7:4817–4828. 2016.PubMed/NCBI

17 

Li QW, Zhou T, Wang F, Jiang M, Liu CB, Zhang KR, Zhou Q, Tian Z and Hu KW: MicroRNA-215 functions as a tumor suppressor and directly targets ZEB2 in human pancreatic cancer. Genet Mol Res. 14:16133–16145. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Chen B, Huang Z, Zhang Y, Chen Y and Li Z: MicroRNA-145 suppresses osteosarcoma metastasis via targeting MMP16. Cell Physiol Biochem. 37:2183–2193. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Arslan D, Bozcuk H, Gunduz S, Tural D, Tattli AM, Uysal M, Goksu SS, Bassorgun Cİ, Koral L, Coskun HS, et al: Survival results and prognostic factors in T4 N0-3 non-small cell lung cancer patients according to the AJCC 7th edition staging system. Asian Pac J Cancer Prev. 15:2465–2472. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Ahmad J, Baig MA, Ali AA, Al-Huqail A, Ibrahim MM and Qureshi MI: Comparative assessment of four RNA extraction methods and modification to obtain high-quality RNA from Parthenium hysterophorus leaf. 3 Biotech. 7:3732017. View Article : Google Scholar : PubMed/NCBI

21 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

22 

Georges SA, Biery MC, Kim SY, Schelter JM, Guo J, Chang AN, Jackson AL, Carleton MO, Linsley PS, Cleary MA and Chau BN: Coordinated regulation of cell cycle transcripts by p53-inducible microRNAs, miR-192 and miR-215. Cancer Res. 68:10105–10112. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Wang YX, Zhang TJ, Yang DQ, Yao DM, Yang L, Zhou JD, Deng ZQ, Ma JC, Guo H, Wen XM, et al: Reduced miR-215 expression predicts poor prognosis in patients with acute myeloid leukemia. Jpn J Clin Oncol. 46:350–356. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Zhou SW, Su BB, Zhou Y, Feng YQ, Guo Y, Wang YX, Qi P and Xu S: Aberrant miR-215 expression is associated with clinical outcome in breast cancer patients. Med Oncol. 31:2592014. View Article : Google Scholar : PubMed/NCBI

25 

Chen Z, Han S, Huang W, Wu J, Liu Y, Cai S, He Y, Wu S and Song W: MicroRNA-215 suppresses cell proliferation, migration and invasion of colon cancer by repressing Yin-Yang 1. Biochem Biophys Res Commun. 479:482–488. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Hou Y, Zhen J, Xu X, Zhen K, Zhu B, Pan R and Zhao C: miR-215 functions as a tumor suppressor and directly targets ZEB2 in human non-small cell lung cancer. Oncol Lett. 10:1985–1992. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Zhang WL, Chen YF, Meng HZ, Du JJ, Luan GN, Wang HQ, Yang MW and Luo ZJ: Role of miR-155 in the regulation of MMP-16 expression in intervertebral disc degeneration. J Orthop Res. 35:1323–1334. 2017. View Article : Google Scholar : PubMed/NCBI

28 

Cao L, Chen C, Zhu H, Gu X, Deng D, Tian X, Liu J and Xiao Q: MMP16 is a marker of poor prognosis in gastric cancer promoting proliferation and invasion. Oncotarget. 7:51865–51874. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Lin F, Wang X, Jie Z, Hong X, Li X, Wang M and Yu Y: Inhibitory effects of miR-146b-5p on cell migration and invasion of pancreatic cancer by targeting MMP16. J Huazhong Univ Sci Technolog Med Sci. 31:509–514. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yao Y, Shen H, Zhou Y, Yang Z and Hu T: MicroRNA-215 suppresses the proliferation, migration and invasion of non-small cell lung carcinoma cells through the downregulation of matrix metalloproteinase-16 expression. Exp Ther Med 15: 3239-3246, 2018.
APA
Yao, Y., Shen, H., Zhou, Y., Yang, Z., & Hu, T. (2018). MicroRNA-215 suppresses the proliferation, migration and invasion of non-small cell lung carcinoma cells through the downregulation of matrix metalloproteinase-16 expression. Experimental and Therapeutic Medicine, 15, 3239-3246. https://doi.org/10.3892/etm.2018.5869
MLA
Yao, Y., Shen, H., Zhou, Y., Yang, Z., Hu, T."MicroRNA-215 suppresses the proliferation, migration and invasion of non-small cell lung carcinoma cells through the downregulation of matrix metalloproteinase-16 expression". Experimental and Therapeutic Medicine 15.4 (2018): 3239-3246.
Chicago
Yao, Y., Shen, H., Zhou, Y., Yang, Z., Hu, T."MicroRNA-215 suppresses the proliferation, migration and invasion of non-small cell lung carcinoma cells through the downregulation of matrix metalloproteinase-16 expression". Experimental and Therapeutic Medicine 15, no. 4 (2018): 3239-3246. https://doi.org/10.3892/etm.2018.5869
Copy and paste a formatted citation
x
Spandidos Publications style
Yao Y, Shen H, Zhou Y, Yang Z and Hu T: MicroRNA-215 suppresses the proliferation, migration and invasion of non-small cell lung carcinoma cells through the downregulation of matrix metalloproteinase-16 expression. Exp Ther Med 15: 3239-3246, 2018.
APA
Yao, Y., Shen, H., Zhou, Y., Yang, Z., & Hu, T. (2018). MicroRNA-215 suppresses the proliferation, migration and invasion of non-small cell lung carcinoma cells through the downregulation of matrix metalloproteinase-16 expression. Experimental and Therapeutic Medicine, 15, 3239-3246. https://doi.org/10.3892/etm.2018.5869
MLA
Yao, Y., Shen, H., Zhou, Y., Yang, Z., Hu, T."MicroRNA-215 suppresses the proliferation, migration and invasion of non-small cell lung carcinoma cells through the downregulation of matrix metalloproteinase-16 expression". Experimental and Therapeutic Medicine 15.4 (2018): 3239-3246.
Chicago
Yao, Y., Shen, H., Zhou, Y., Yang, Z., Hu, T."MicroRNA-215 suppresses the proliferation, migration and invasion of non-small cell lung carcinoma cells through the downregulation of matrix metalloproteinase-16 expression". Experimental and Therapeutic Medicine 15, no. 4 (2018): 3239-3246. https://doi.org/10.3892/etm.2018.5869
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