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Advanced oxidation protein products inhibit the autophagy of renal tubular epithelial cells

  • Authors:
    • Jun Zhang
    • Xiaohong Xiang
    • Shuangshuang Shu
    • Cuiling Zhang
    • Yuling Liang
    • Tingting Jiang
    • Wenying Zhang
    • Tingting Guo
    • Xiujie Liang
    • Xun Tang
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong 510280, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3908-3916
    |
    Published online on: February 15, 2018
       https://doi.org/10.3892/etm.2018.5875
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Abstract

It is well known that autophagy serves a crucial role in renal tubular epithelial cell (RTEC) injury in the pathogenesis of chronic kidney disease (CKD). The accumulation of advanced oxidation protein products (AOPPs) also participates in the progression of CKD. However, the effects of AOPPs on autophagy remain unknown. To clarify the underlying mechanism of RTEC injury in CKD, the effect of AOPPs on HK‑2 cells, an RTEC cell line, was investigated. The results of the present study revealed that AOPP exposure downregulated the expression of B‑cell lymphoma‑2‑interacting myosin‑like coiled‑coil protein 1, reduced the conversion of microtubule‑associated proteins 1 light chain 3B (LC3)‑I to LC3‑II and the formation of autophagosomes, and lead to an accumulation of p62. These results suggest that AOPPs may inhibit the autophagic activity of HK‑2 cells. Furthermore, the aforementioned changes were mediated by the AOPP‑phosphorylated phosphoinositide 3‑kinase3‑kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway; this was verified by treatment with LY294002, a PI3K inhibitor, which reversed the AOPP‑induced changes. The present study also demonstrated that the activation of autophagy with rapamycin led to an improvement in the AOPP‑induced overexpression of kidney injury molecule 1 and neutrophil gelatinase‑associated lipocalin, two biomarkers of RTEC injury, whereas inhibiting autophagy with chloroquine further increased their expression during AOPP treatment. Collectively, these results indicate that AOPPs may inhibit autophagy in RTECs via activation of the PI3K/AKT/mTOR pathway and that autophagy inhibition serves a role in AOPP-induced RTEC injury.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang J, Xiang X, Shu S, Zhang C, Liang Y, Jiang T, Zhang W, Guo T, Liang X, Tang X, Tang X, et al: Advanced oxidation protein products inhibit the autophagy of renal tubular epithelial cells. Exp Ther Med 15: 3908-3916, 2018.
APA
Zhang, J., Xiang, X., Shu, S., Zhang, C., Liang, Y., Jiang, T. ... Tang, X. (2018). Advanced oxidation protein products inhibit the autophagy of renal tubular epithelial cells. Experimental and Therapeutic Medicine, 15, 3908-3916. https://doi.org/10.3892/etm.2018.5875
MLA
Zhang, J., Xiang, X., Shu, S., Zhang, C., Liang, Y., Jiang, T., Zhang, W., Guo, T., Liang, X., Tang, X."Advanced oxidation protein products inhibit the autophagy of renal tubular epithelial cells". Experimental and Therapeutic Medicine 15.4 (2018): 3908-3916.
Chicago
Zhang, J., Xiang, X., Shu, S., Zhang, C., Liang, Y., Jiang, T., Zhang, W., Guo, T., Liang, X., Tang, X."Advanced oxidation protein products inhibit the autophagy of renal tubular epithelial cells". Experimental and Therapeutic Medicine 15, no. 4 (2018): 3908-3916. https://doi.org/10.3892/etm.2018.5875
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Xiang X, Shu S, Zhang C, Liang Y, Jiang T, Zhang W, Guo T, Liang X, Tang X, Tang X, et al: Advanced oxidation protein products inhibit the autophagy of renal tubular epithelial cells. Exp Ther Med 15: 3908-3916, 2018.
APA
Zhang, J., Xiang, X., Shu, S., Zhang, C., Liang, Y., Jiang, T. ... Tang, X. (2018). Advanced oxidation protein products inhibit the autophagy of renal tubular epithelial cells. Experimental and Therapeutic Medicine, 15, 3908-3916. https://doi.org/10.3892/etm.2018.5875
MLA
Zhang, J., Xiang, X., Shu, S., Zhang, C., Liang, Y., Jiang, T., Zhang, W., Guo, T., Liang, X., Tang, X."Advanced oxidation protein products inhibit the autophagy of renal tubular epithelial cells". Experimental and Therapeutic Medicine 15.4 (2018): 3908-3916.
Chicago
Zhang, J., Xiang, X., Shu, S., Zhang, C., Liang, Y., Jiang, T., Zhang, W., Guo, T., Liang, X., Tang, X."Advanced oxidation protein products inhibit the autophagy of renal tubular epithelial cells". Experimental and Therapeutic Medicine 15, no. 4 (2018): 3908-3916. https://doi.org/10.3892/etm.2018.5875
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