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Correlation between the expression of Drp1 in vascular endothelial cells and inflammatory factors in hypertension rats

  • Authors:
    • Xinghui Liu
    • Hongwen Tan
    • Xiaoqiao Liu
    • Qiang Wu
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Guizhou Provincial People's Hospital, Guiyang, Guizhou 550002, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3892-3898
    |
    Published online on: February 27, 2018
       https://doi.org/10.3892/etm.2018.5899
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Abstract

The objective of this study was to investigate the expression level of dynamin-related protein 1 (Drp1) in vascular endothelium of hypertension rats and its correlation with expression of inflammatory factors. Twenty spontaneous hypertension rats (SHR) were randomly divided into SHR group (n=10) and inhibition group (MD group, n=10), and the Sprague Dawley rats were enrolled as the control group (C group, n=10). For rats in the MD group, Mdivi-1, a mitochondrial division inhibitor, was given in dosage of 25 mg/kg. After 4 weeks of administration, blood pressure was measured via tail-artery blood pressure measurement. The blood samples collected from the abdominal aorta of rats were used to assay the C-reaction protein (CRP) concentration in serum through radioimmunoassay. Hematoxylin and eosin (H&E) staining was performed for sections of thoracic aorta for morphological observation and measurement of medial thickness. Enzyme-linked immunosorbant assay (ELISA), semi-quantitative real-time polymerase chain reaction (RT-PCR) and western blotting was carried out for detecting the expression levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). Drp1 and monocyte chemotactic protein 1 (MCP-1). After 4 weeks of drug administration, the blood pressure in the MD group was significantly higher (P<0.01). The medial thickness of the thoracic aorta in the MD group was significantly decreased in comparison with the SHR group (P<0.01). The results of ELISA showed that compared with the SHR group, the expression levels of IL-6 and TNF-α in the MD group were remarkably decreased (P<0.01). Semi-quantitative RT-PCR results indicated that the mRNA expression levels of Drp1 and MCP-1 in the MD group were significantly lower than those in the SHR group (P<0.05). In the SHR rats, after administration of Mdivi-1, the expression of Drp1 is decreased, which contributes to the alleviation in inflammatory reactions and protects the vessels in SHR rats.
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Copy and paste a formatted citation
Spandidos Publications style
Liu X, Tan H, Liu X and Wu Q: Correlation between the expression of Drp1 in vascular endothelial cells and inflammatory factors in hypertension rats. Exp Ther Med 15: 3892-3898, 2018.
APA
Liu, X., Tan, H., Liu, X., & Wu, Q. (2018). Correlation between the expression of Drp1 in vascular endothelial cells and inflammatory factors in hypertension rats. Experimental and Therapeutic Medicine, 15, 3892-3898. https://doi.org/10.3892/etm.2018.5899
MLA
Liu, X., Tan, H., Liu, X., Wu, Q."Correlation between the expression of Drp1 in vascular endothelial cells and inflammatory factors in hypertension rats". Experimental and Therapeutic Medicine 15.4 (2018): 3892-3898.
Chicago
Liu, X., Tan, H., Liu, X., Wu, Q."Correlation between the expression of Drp1 in vascular endothelial cells and inflammatory factors in hypertension rats". Experimental and Therapeutic Medicine 15, no. 4 (2018): 3892-3898. https://doi.org/10.3892/etm.2018.5899
Copy and paste a formatted citation
x
Spandidos Publications style
Liu X, Tan H, Liu X and Wu Q: Correlation between the expression of Drp1 in vascular endothelial cells and inflammatory factors in hypertension rats. Exp Ther Med 15: 3892-3898, 2018.
APA
Liu, X., Tan, H., Liu, X., & Wu, Q. (2018). Correlation between the expression of Drp1 in vascular endothelial cells and inflammatory factors in hypertension rats. Experimental and Therapeutic Medicine, 15, 3892-3898. https://doi.org/10.3892/etm.2018.5899
MLA
Liu, X., Tan, H., Liu, X., Wu, Q."Correlation between the expression of Drp1 in vascular endothelial cells and inflammatory factors in hypertension rats". Experimental and Therapeutic Medicine 15.4 (2018): 3892-3898.
Chicago
Liu, X., Tan, H., Liu, X., Wu, Q."Correlation between the expression of Drp1 in vascular endothelial cells and inflammatory factors in hypertension rats". Experimental and Therapeutic Medicine 15, no. 4 (2018): 3892-3898. https://doi.org/10.3892/etm.2018.5899
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