Open Access

Protective effect of Atriplex suberecta extract against oxidative and apoptotic hepatotoxicity

  • Authors:
    • Mohammad K. Parvez
    • Ahmad H. Arbab
    • Mohammed S. Al‑Dosari
    • Adnan J. Al‑Rehaily
    • Perwez Alam
    • Khalid E. Ibrahim
    • Mansour S. Alsaid
    • Syed Rafatullah
  • View Affiliations

  • Published online on: March 2, 2018     https://doi.org/10.3892/etm.2018.5919
  • Pages: 3883-3891
  • Copyright: © Parvez et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Atriplex suberecta I. Verd is a known phytomedicinal species of Atriplex; however, studies into its bioactivity remain inconclusive. The in vitro and in vivo antioxidative and hepatoprotective potential of A. suberecta ethanol‑extract (ASEE) was assessed in the present study. 1,1‑diphenyl‑2‑picrylhydrazyl radical scavenging and β‑carotene bleaching assays revealed that ASEE possesses free radical scavenging and anti‑lipid peroxidative activities. These results were supported by the in vitro protection of HepG2 hepatoblastoma cells via abating 2,7‑dichlorofluorescein‑activated oxidative and apoptotic molecules (caspase‑3/‑7). In carbon tetrachloride‑treated rats, the oral administration of ASEE significantly normalized serum biomarkers of liver function (serum glutamate oxaloacetate, serum pyruvate transaminase, alkaline phosphatase, γ‑glutamyl transferase and bilirubin) and the lipid profile (total cholesterol, high‑density lipoprotein, low‑density lipoprotein, triglycerides and malondialdehyde), including tissue non‑protein sulfhydryl and total protein levels. These results were also supported by liver histopathology, which demonstrated that the therapeutic effect of ASEE was comparable to silymarin. Furthermore, phytochemical analysis of ASEE revealed the presence of flavonoids, alkaloids, tannins and saponins. Rutin, an antioxidant flavonoid, was identified using the validated high‑performance thin‑layer chromatography method. In conclusion, this is the first report on the therapeutic potential of A. suberecta against chemical‑induced oxidative stress and liver damage.
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April-2018
Volume 15 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Parvez MK, Arbab AH, Al‑Dosari MS, Al‑Rehaily AJ, Alam P, Ibrahim KE, Alsaid MS and Rafatullah S: Protective effect of Atriplex suberecta extract against oxidative and apoptotic hepatotoxicity. Exp Ther Med 15: 3883-3891, 2018
APA
Parvez, M.K., Arbab, A.H., Al‑Dosari, M.S., Al‑Rehaily, A.J., Alam, P., Ibrahim, K.E. ... Rafatullah, S. (2018). Protective effect of Atriplex suberecta extract against oxidative and apoptotic hepatotoxicity. Experimental and Therapeutic Medicine, 15, 3883-3891. https://doi.org/10.3892/etm.2018.5919
MLA
Parvez, M. K., Arbab, A. H., Al‑Dosari, M. S., Al‑Rehaily, A. J., Alam, P., Ibrahim, K. E., Alsaid, M. S., Rafatullah, S."Protective effect of Atriplex suberecta extract against oxidative and apoptotic hepatotoxicity". Experimental and Therapeutic Medicine 15.4 (2018): 3883-3891.
Chicago
Parvez, M. K., Arbab, A. H., Al‑Dosari, M. S., Al‑Rehaily, A. J., Alam, P., Ibrahim, K. E., Alsaid, M. S., Rafatullah, S."Protective effect of Atriplex suberecta extract against oxidative and apoptotic hepatotoxicity". Experimental and Therapeutic Medicine 15, no. 4 (2018): 3883-3891. https://doi.org/10.3892/etm.2018.5919