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MicroRNA-34a regulates proliferation and apoptosis of gastric cancer cells by targeting silent information regulator 1

  • Authors:
    • Xiaojing Deng
    • Hailun Zheng
    • Dapeng Li
    • Yongju Xue
    • Qizhi Wang
    • Shanjun Yan
    • Yu Zhu
    • Min Deng
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, P.R. China
    Copyright: © Deng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3705-3714
    |
    Published online on: March 2, 2018
       https://doi.org/10.3892/etm.2018.5920
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Abstract

The present study aimed to identify whether microRNA (miRNA/miR)‑34a regulates the proliferation and apoptosis of gastric cancer cells by targeting silent information regulator 1 (SIRT1). The expression of miR‑34a and SIRT1 and cell viability was investigated in gastric cancer cells. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was applied to determine miR‑34a expression in gastric adenocarcinoma, normal pericarcinomatous tissues, human normal gastric mucosa epithelial cell line GES and various gastric cancer cell strains. A bioinformatics method was then used to predict the target gene of miR‑34a. A human miR‑34a over expression lentiviral vector system was constructed and then used for transfection of the gastric cancer cell line SCG‑7901 to determine the expression of SIRT1 mRNA and SIRT1 protein using RT‑qPCR and western blot analysis. The MTT method and flow cytometry was used to measure cell proliferation and apoptosis. The relative expression of miR‑34a in gastric cancer tissues was significantly decreased compared with that in normal tissues (P<0.01). miR‑34a expression was also significantly decreased in low differentiated N2, N3 gastric cancer tissues (P<0.01). However, tumor size and filtration degree were not significantly associated with miR‑34a expression. The relative expression of miR‑34a was decreased in gastric cancer cells, especially in the SGC‑7901 cell line (P<0.01) compared with the GES group. The relative expression of SIRT1 protein was decreased in the miR‑34a group compared with the negative control (P<0.01). The rate of proliferation was significantly decreased, whereas the rate of apoptosis was significantly increased in the miR‑34a group compared with the NC group (P<0.01). Therefore, the present results suggested that miRNA‑34a serves a pivotal role in gastric cancer as a cancer suppressor gene by targeting SIRT1 to regulate the proliferation and apoptosis of gastric cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Deng X, Zheng H, Li D, Xue Y, Wang Q, Yan S, Zhu Y and Deng M: MicroRNA-34a regulates proliferation and apoptosis of gastric cancer cells by targeting silent information regulator 1. Exp Ther Med 15: 3705-3714, 2018.
APA
Deng, X., Zheng, H., Li, D., Xue, Y., Wang, Q., Yan, S. ... Deng, M. (2018). MicroRNA-34a regulates proliferation and apoptosis of gastric cancer cells by targeting silent information regulator 1. Experimental and Therapeutic Medicine, 15, 3705-3714. https://doi.org/10.3892/etm.2018.5920
MLA
Deng, X., Zheng, H., Li, D., Xue, Y., Wang, Q., Yan, S., Zhu, Y., Deng, M."MicroRNA-34a regulates proliferation and apoptosis of gastric cancer cells by targeting silent information regulator 1". Experimental and Therapeutic Medicine 15.4 (2018): 3705-3714.
Chicago
Deng, X., Zheng, H., Li, D., Xue, Y., Wang, Q., Yan, S., Zhu, Y., Deng, M."MicroRNA-34a regulates proliferation and apoptosis of gastric cancer cells by targeting silent information regulator 1". Experimental and Therapeutic Medicine 15, no. 4 (2018): 3705-3714. https://doi.org/10.3892/etm.2018.5920
Copy and paste a formatted citation
x
Spandidos Publications style
Deng X, Zheng H, Li D, Xue Y, Wang Q, Yan S, Zhu Y and Deng M: MicroRNA-34a regulates proliferation and apoptosis of gastric cancer cells by targeting silent information regulator 1. Exp Ther Med 15: 3705-3714, 2018.
APA
Deng, X., Zheng, H., Li, D., Xue, Y., Wang, Q., Yan, S. ... Deng, M. (2018). MicroRNA-34a regulates proliferation and apoptosis of gastric cancer cells by targeting silent information regulator 1. Experimental and Therapeutic Medicine, 15, 3705-3714. https://doi.org/10.3892/etm.2018.5920
MLA
Deng, X., Zheng, H., Li, D., Xue, Y., Wang, Q., Yan, S., Zhu, Y., Deng, M."MicroRNA-34a regulates proliferation and apoptosis of gastric cancer cells by targeting silent information regulator 1". Experimental and Therapeutic Medicine 15.4 (2018): 3705-3714.
Chicago
Deng, X., Zheng, H., Li, D., Xue, Y., Wang, Q., Yan, S., Zhu, Y., Deng, M."MicroRNA-34a regulates proliferation and apoptosis of gastric cancer cells by targeting silent information regulator 1". Experimental and Therapeutic Medicine 15, no. 4 (2018): 3705-3714. https://doi.org/10.3892/etm.2018.5920
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