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Article Open Access

Application values of miR-194 and miR-29 in the diagnosis and prognosis of gastric cancer

  • Authors:
    • Xin Zhao
    • Yunxia Hou
    • Zhongzhen Tuo
    • Fangmeng Wei
  • View Affiliations / Copyright

    Affiliations: Medical Research and Laboratory Diagnostic Center, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250013, P.R. China, Department of Clinical Laboratory, Weifang Yidu Central Hospital, Weifang, Shandong 262500, P.R. China, Department of Clinical Laboratory, Weifang People's Hospital, Weifang, Shandong 261041, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4179-4184
    |
    Published online on: March 6, 2018
       https://doi.org/10.3892/etm.2018.5931
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Abstract

The object of this study was to analyze the expression of miR-194 and miR-29 in gastric cancer and their roles in the regulation of malignant phenotype of gastric cancer cells, and to explore the application value of miR-194 and miR-29 in diagnosis and prognosis of gastric cancer. Tumor tissue and adjacent healthy tissue of 165 gastric cancer patients diagnosed by pathologic examinations were collected. Expression of miR-194 and miR-29 in the tissues was detected by RT-PCR. The relationship between miR-194 and miR-29 expression and clinical data was analyzed. SGC7901 cells were treated with miR-194 and miR-29 mimics, respectively. Effects of miR-194 and miR-29 on proliferation and invasion of SGC7901 cells were investigated. Expression levels of miR-194 and miR-29 in tumor tissue were lower than those in adjacent tissues (P<0.001). There was no significant difference in expression level of miR-194 and miR-29 in cancer tissues derived from gastric cancer patients in different age and gender groups (P>0.05). Expression of miR-194 and miR-29 in tumor tissue was closely related to TNM stage, differentiation degree of cancer cells and lymph node metastasis (P<0.05). Proliferation and migration of SGC7901 cells were significantly inhibited by miR-194 mimic and miR-29 mimic transfection (P<0.05). miR-194 and miR-29 are downregulated in gastric cancer, and the expression levels of miR-194 and miR-29 were closely related to tumor differentiation and metastasis. Overexpression of miR-194 and miR-29 significantly inhibited the proliferation and migration of gastric cancer. The detection of the expression of miR-194 and miR-29 can provide basis for the diagnosis and prognosis of gastric cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao X, Hou Y, Tuo Z and Wei F: Application values of miR-194 and miR-29 in the diagnosis and prognosis of gastric cancer. Exp Ther Med 15: 4179-4184, 2018.
APA
Zhao, X., Hou, Y., Tuo, Z., & Wei, F. (2018). Application values of miR-194 and miR-29 in the diagnosis and prognosis of gastric cancer. Experimental and Therapeutic Medicine, 15, 4179-4184. https://doi.org/10.3892/etm.2018.5931
MLA
Zhao, X., Hou, Y., Tuo, Z., Wei, F."Application values of miR-194 and miR-29 in the diagnosis and prognosis of gastric cancer". Experimental and Therapeutic Medicine 15.5 (2018): 4179-4184.
Chicago
Zhao, X., Hou, Y., Tuo, Z., Wei, F."Application values of miR-194 and miR-29 in the diagnosis and prognosis of gastric cancer". Experimental and Therapeutic Medicine 15, no. 5 (2018): 4179-4184. https://doi.org/10.3892/etm.2018.5931
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao X, Hou Y, Tuo Z and Wei F: Application values of miR-194 and miR-29 in the diagnosis and prognosis of gastric cancer. Exp Ther Med 15: 4179-4184, 2018.
APA
Zhao, X., Hou, Y., Tuo, Z., & Wei, F. (2018). Application values of miR-194 and miR-29 in the diagnosis and prognosis of gastric cancer. Experimental and Therapeutic Medicine, 15, 4179-4184. https://doi.org/10.3892/etm.2018.5931
MLA
Zhao, X., Hou, Y., Tuo, Z., Wei, F."Application values of miR-194 and miR-29 in the diagnosis and prognosis of gastric cancer". Experimental and Therapeutic Medicine 15.5 (2018): 4179-4184.
Chicago
Zhao, X., Hou, Y., Tuo, Z., Wei, F."Application values of miR-194 and miR-29 in the diagnosis and prognosis of gastric cancer". Experimental and Therapeutic Medicine 15, no. 5 (2018): 4179-4184. https://doi.org/10.3892/etm.2018.5931
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