Open Access

Application values of miR-194 and miR-29 in the diagnosis and prognosis of gastric cancer

  • Authors:
    • Xin Zhao
    • Yunxia Hou
    • Zhongzhen Tuo
    • Fangmeng Wei
  • View Affiliations

  • Published online on: March 6, 2018     https://doi.org/10.3892/etm.2018.5931
  • Pages: 4179-4184
  • Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The object of this study was to analyze the expression of miR-194 and miR-29 in gastric cancer and their roles in the regulation of malignant phenotype of gastric cancer cells, and to explore the application value of miR-194 and miR-29 in diagnosis and prognosis of gastric cancer. Tumor tissue and adjacent healthy tissue of 165 gastric cancer patients diagnosed by pathologic examinations were collected. Expression of miR-194 and miR-29 in the tissues was detected by RT-PCR. The relationship between miR-194 and miR-29 expression and clinical data was analyzed. SGC7901 cells were treated with miR-194 and miR-29 mimics, respectively. Effects of miR-194 and miR-29 on proliferation and invasion of SGC7901 cells were investigated. Expression levels of miR-194 and miR-29 in tumor tissue were lower than those in adjacent tissues (P<0.001). There was no significant difference in expression level of miR-194 and miR-29 in cancer tissues derived from gastric cancer patients in different age and gender groups (P>0.05). Expression of miR-194 and miR-29 in tumor tissue was closely related to TNM stage, differentiation degree of cancer cells and lymph node metastasis (P<0.05). Proliferation and migration of SGC7901 cells were significantly inhibited by miR-194 mimic and miR-29 mimic transfection (P<0.05). miR-194 and miR-29 are downregulated in gastric cancer, and the expression levels of miR-194 and miR-29 were closely related to tumor differentiation and metastasis. Overexpression of miR-194 and miR-29 significantly inhibited the proliferation and migration of gastric cancer. The detection of the expression of miR-194 and miR-29 can provide basis for the diagnosis and prognosis of gastric cancer.
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May-2018
Volume 15 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Zhao X, Hou Y, Tuo Z and Wei F: Application values of miR-194 and miR-29 in the diagnosis and prognosis of gastric cancer. Exp Ther Med 15: 4179-4184, 2018
APA
Zhao, X., Hou, Y., Tuo, Z., & Wei, F. (2018). Application values of miR-194 and miR-29 in the diagnosis and prognosis of gastric cancer. Experimental and Therapeutic Medicine, 15, 4179-4184. https://doi.org/10.3892/etm.2018.5931
MLA
Zhao, X., Hou, Y., Tuo, Z., Wei, F."Application values of miR-194 and miR-29 in the diagnosis and prognosis of gastric cancer". Experimental and Therapeutic Medicine 15.5 (2018): 4179-4184.
Chicago
Zhao, X., Hou, Y., Tuo, Z., Wei, F."Application values of miR-194 and miR-29 in the diagnosis and prognosis of gastric cancer". Experimental and Therapeutic Medicine 15, no. 5 (2018): 4179-4184. https://doi.org/10.3892/etm.2018.5931