Downregulation of microRNA‑193‑3p inhibits the progression of intrahepatic cholangiocarcinoma cells by upregulating TGFBR3

Han,Yu‑Long; Yin,Jia‑Jun; Cong,Jian‑Jun

doi:10.3892/etm.2018.5958

Downregulation of microRNA‑193‑3p inhibits the progression of intrahepatic cholangiocarcinoma cells by upregulating TGFBR3

Authors:
- Yu‑Long Han
- Jia‑Jun Yin
- Jian‑Jun Cong
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Affiliations: Department of Hepatobiliary Laparoscopic Surgery Ward, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning 116001, P.R. China
Published online on: March 14, 2018 https://doi.org/10.3892/etm.2018.5958
Pages: 4508-4514

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Abstract

The aim of this study was to investigate the function of microRNA (miR)‑193‑3p in human intrahepatic cholangiocarcinoma (ICC) tissues and cells. To evaluate whether miR‑193‑3p was aberrantly upregulated, we used reverse transcription‑quantitative polymerase chain reaction to detect the level of miR‑193‑3p in ICC tissues and ICC‑9810 cells. The effects of miR‑193‑3p downregulation on ICC cell proliferation, migration and invasion were also measured by MTT, wound‑healing and Transwell assays. Additionally, transforming growth factor‑β receptor type 3 (TGFBR3) was investigated as a direct target of miR‑193‑3p by dual‑luciferase reporter assays and western blot analyses. The results demonstrated that miR‑193‑3p was aberrantly upregulated in ICC tissues and cell lines. Furthermore, TGFBR3 was confirmed to be a target of miR‑193‑3p in ICC and was notably upregulated by miR‑193‑3p knockdown in the ICC‑9810 cells. The knockdown of miR‑193‑3p also exerted direct inhibitory effects on the proliferation, migration and invasion of the ICC‑9810 cells. Therefore, we present evidence that miR‑193‑3p plays a key role in promoting human ICC by regulating TGFBR3.

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