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Homocysteine induces oxidative stress to damage trabecular meshwork cells

  • Authors:
    • Zhi‑Peng You
    • Yue‑Zhi Zhang
    • Yu‑Lan Zhang
    • Lu Shi
    • Ke Shi
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China
    Copyright: © You et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4379-4385
    |
    Published online on: March 19, 2018
       https://doi.org/10.3892/etm.2018.5961
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Abstract

The aim of the present study was to investigate the effect of homocysteine (Hcy) in on human trabecular meshwork cells (HTMCs). A total of 41 patients with primary open‑angle glaucoma (POAG) and 53 patients with senile cataracts (control group) were recruited. Plasma and aqueous humor samples were collected and the Hcy concentrations were determined using enzymatic cycling assays. In cell experiments, normal HTMCs were passaged and randomly divided into a blank control group, a normal HTMC group and experimental groups, which were treated with different concentrations of Hcy. The HTMC activities were detected using the Cell Counting Kit‑8 method and the HTMC mitochondrial membrane potential (MMP) was detected using JC‑1 staining. Reactive oxygen species (ROS) released by trabecular meshwork cells was detected using flow cytometry and superoxide dismjutase‑1 (SOD1) expression was detected using immunoblotting. The results revealed that the concentration of Hcy in the plasma and aqueous humor of the POAG group (14.44±0.86 and 1.60±0.27 µmol/l, respectively) was significantly higher compared with the control group (10.82±0.29 and 0.69±0.39 µmol/l). All tested concentrations (30, 100, 300 and 1,000 µmol/l) of Hcy reduced the MMP in HTMCs and inhibited HTMC proliferation in a dose‑dependent manner. ROS production by HTMCs significantly increased with increased concentrations of Hcy, whereas SOD1 expression significantly decreased in a dose‑dependent manner. In summary, patients with POAG were demonstrated to have increased concentrations of Hcy in the plasma and aqueous humor. High concentrations of Hcy in HTMCs induced an oxidative stress state, thereby further inhibiting HTMC proliferation. The results of the present study demonstrate that Hcy may be a potential treatment target in patients with POAG.
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Copy and paste a formatted citation
Spandidos Publications style
You ZP, Zhang YZ, Zhang YL, Shi L and Shi K: Homocysteine induces oxidative stress to damage trabecular meshwork cells. Exp Ther Med 15: 4379-4385, 2018.
APA
You, Z., Zhang, Y., Zhang, Y., Shi, L., & Shi, K. (2018). Homocysteine induces oxidative stress to damage trabecular meshwork cells. Experimental and Therapeutic Medicine, 15, 4379-4385. https://doi.org/10.3892/etm.2018.5961
MLA
You, Z., Zhang, Y., Zhang, Y., Shi, L., Shi, K."Homocysteine induces oxidative stress to damage trabecular meshwork cells". Experimental and Therapeutic Medicine 15.5 (2018): 4379-4385.
Chicago
You, Z., Zhang, Y., Zhang, Y., Shi, L., Shi, K."Homocysteine induces oxidative stress to damage trabecular meshwork cells". Experimental and Therapeutic Medicine 15, no. 5 (2018): 4379-4385. https://doi.org/10.3892/etm.2018.5961
Copy and paste a formatted citation
x
Spandidos Publications style
You ZP, Zhang YZ, Zhang YL, Shi L and Shi K: Homocysteine induces oxidative stress to damage trabecular meshwork cells. Exp Ther Med 15: 4379-4385, 2018.
APA
You, Z., Zhang, Y., Zhang, Y., Shi, L., & Shi, K. (2018). Homocysteine induces oxidative stress to damage trabecular meshwork cells. Experimental and Therapeutic Medicine, 15, 4379-4385. https://doi.org/10.3892/etm.2018.5961
MLA
You, Z., Zhang, Y., Zhang, Y., Shi, L., Shi, K."Homocysteine induces oxidative stress to damage trabecular meshwork cells". Experimental and Therapeutic Medicine 15.5 (2018): 4379-4385.
Chicago
You, Z., Zhang, Y., Zhang, Y., Shi, L., Shi, K."Homocysteine induces oxidative stress to damage trabecular meshwork cells". Experimental and Therapeutic Medicine 15, no. 5 (2018): 4379-4385. https://doi.org/10.3892/etm.2018.5961
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