Open Access

Upregulation of c-mesenchymal epithelial transition expression and RAS mutations are associated with late lung metastasis and poor prognosis in colorectal carcinoma

  • Authors:
    • Jianhua Liu
    • Weiqiang Zeng
    • Chengzhi Huang
    • Junjiang Wang
    • Lishu Xu
    • Dong Ma
  • View Affiliations

  • Published online on: March 19, 2018     https://doi.org/10.3892/etm.2018.5966
  • Pages: 4229-4242
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate whether c-mesenchymal epithelial transition factor (C‑MET) overexpression combined with RAS (including KRAS, NRAS and HRAS) or BRAF mutations were associated with late distant metastases and the prognosis of patients with colorectal cancer (CRC). A total of 374 patients with stage III CRC were classified into 4 groups based on RAS/BRAF and C‑MET status for comprehensive analysis. Mutations in RAS/BRAF were determined using Sanger sequencing and C‑MET expression was examined using immunohistochemistry. The associations between RAS/BRAF mutations in combination with C‑MET overexpression and clinicopathological variables including survival were evaluated. In addition, their predictive value for late distant metastases were statistically analyzed via logistic regression and receiver operating characteristic analysis. Among 374 patients, mutations in KRAS, NRAS, HRAS, BRAF and C‑MET overexpression were observed in 43.9, 2.4, 0.3, 5.9 and 71.9% of cases, respectively. Considering RAS/BRAF mutations and C‑MET overexpression, vascular invasion (P=0.001), high carcino‑embryonic antigen level (P=0.031) and late distant metastases (P<0.001) were more likely to occur in patients of group 4. Furthermore, survival analyses revealed RAS/BRAF mutations may have a more powerful impact on survival than C‑MET overexpression, although they were both predictive factors for adverse prognosis. Further logistic regression suggested that RAS/BRAF mutations and C‑MET overexpression may predict late distant metastases. In conclusion, RAS/BRAF mutations and C‑MET overexpression may serve as predictive indicators for metastatic behavior and poor prognosis of CRC.
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May-2018
Volume 15 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu J, Zeng W, Huang C, Wang J, Xu L and Ma D: Upregulation of c-mesenchymal epithelial transition expression and RAS mutations are associated with late lung metastasis and poor prognosis in colorectal carcinoma. Exp Ther Med 15: 4229-4242, 2018
APA
Liu, J., Zeng, W., Huang, C., Wang, J., Xu, L., & Ma, D. (2018). Upregulation of c-mesenchymal epithelial transition expression and RAS mutations are associated with late lung metastasis and poor prognosis in colorectal carcinoma. Experimental and Therapeutic Medicine, 15, 4229-4242. https://doi.org/10.3892/etm.2018.5966
MLA
Liu, J., Zeng, W., Huang, C., Wang, J., Xu, L., Ma, D."Upregulation of c-mesenchymal epithelial transition expression and RAS mutations are associated with late lung metastasis and poor prognosis in colorectal carcinoma". Experimental and Therapeutic Medicine 15.5 (2018): 4229-4242.
Chicago
Liu, J., Zeng, W., Huang, C., Wang, J., Xu, L., Ma, D."Upregulation of c-mesenchymal epithelial transition expression and RAS mutations are associated with late lung metastasis and poor prognosis in colorectal carcinoma". Experimental and Therapeutic Medicine 15, no. 5 (2018): 4229-4242. https://doi.org/10.3892/etm.2018.5966