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TGF-β3 reduces apoptosis in ischemia-induced adipose-derived stem cells by enhancing DNA repair

  • Authors:
    • Fan Wu
    • Haiwen Ye
    • Junfeng Lin
    • Yaodong Xu
    • Zhuasong Zhang
    • Hao Xiong
    • Maojin Laing
    • Yiqing Zhen
    • Suijun Chen
  • View Affiliations / Copyright

    Affiliations: Department of Otorhinolaryngology, Sun Yat‑sen Memorial Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510000, P.R. China
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 4.0].
  • Pages: 4400-4408
    |
    Published online on: March 21, 2018
       https://doi.org/10.3892/etm.2018.5980
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Abstract

Adipose‑derived stem cells (ADSCs) possess good proliferative and differentiative abilities, making then a promising candidate for the treatment of cartilage defects. However, local ischemia often causes apoptosis in ADSCs. Transforming growth factor‑β3 (TGF‑β3) is often used as a chondrogenic differentiation cytokine whose function in apoptosis is unclear. The aim of the present study was to investigate the role of TGF‑β3 in ischemia‑induced ADSC apoptosis. In the present study, the phenotypes and multipotent differentiation properties of human ADSCs at passage 3 were analyzed using flow cytometry and cytochemical staining. ADSCs were cultured in a serum‑ and glucose‑free medium under hypoxic conditions with or without exogenous TGF‑β3 treatment. The apoptosis rate was measured using a TUNEL array and Annexin V/propidium iodide staining. The expression of apoptosis‑associated proteins was measured using western blotting. The results revealed ADSCs cultured in normal condition have multi‑lineage differentiation potential and high levels of cluster of differentiation (CD)29, CD44 and CD105 expression. Furthermore, ADSCs weakly express CD14, CD34 and CD45, with strong clone formation and migration abilities. Serum deprivation under hypoxic conditions resulted in mitochondria‑mediated apoptosis in ADSCs, which was attenuated by exogenous TGF‑β3 treatment via upregulation of poly ADP‑ribose polymerase (PARP). The results of the present study indicate that TGF‑β3 is able to protect ADSCs from ischemia‑induced apoptosis via PARP‑associated DNA damage repair.
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View References

1 

Hunziker EB: Articular cartilage repair: Basic science and clinical progress. A review of the current status and prospects. Osteoarthritis Cartilage. 10:432–463. 2002. View Article : Google Scholar : PubMed/NCBI

2 

Rodriguez-Merchan EC: Regeneration of articular cartilage of the knee. Rheumatol Int. 33:837–845. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Shimomura K, Ando W, Fujie H, Hart DA, Yoshikawa H and Nakamura N: Scaffold-free tissue engineering for injured joint surface restoration. J Exp Orthop. 5:22018. View Article : Google Scholar : PubMed/NCBI

4 

Sterodimas A, de Faria J, Nicaretta B and Pitanguy I: Tissue engineering with adipose-derived stem cells (ADSCs): Current and future applications. J Plast Reconstr Aesthet Surg. 63:1886–1892. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Zheng DY, Dan Y, Yang SH, Liu GH, Shao ZW, Yang C, Xiao BJ, Liu X, Wu S, Zhang T and Chu PK: Controlled chondrogenesis from adipose-derived stem cells by recombinant transforming growth factor-β3 fusion protein in peptide scaffolds. Acta Biomater. 11:191–203. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Cui Z, Shen L, Lin Y, Wang S, Zheng D and Tan Q: Inhibition of oxygen-glucose deprivation-induced apoptosis of human adipose-derived stem cells by genetic modification with antiapoptotic protein bcl-2. Aesthetic Plast Surg. 38:779–787. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Zhu S, Chen P, Wu Y, Xiong S, Sun H, Xia Q, Shi L, Liu H and Ouyang HW: Programmed application of transforming growth factor β3 and rac1 inhibitor NSC23766 committed hyaline cartilage differentiation of adipose-derived stem cells for osteochondral defect repair. Stem Cells Transl Med. 3:1242–1251. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Laverty HG, Wakefield LM, Occleston NL, O'Kane S and Ferguson MW: TGF-beta3 and cancer: A review. Cytokine Growth Factor Rev. 20:305–317. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Shen B, Wei A, Tao H, Diwan AD and Ma DD: BMP-2 enhances TGF-beta3-mediated chondrogenic differentiation of human bone marrow multipotent mesenchymal stromal cells in alginate bead culture. Tissue Eng Part A. 15:1311–1320. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Dünker N, Schmitt K and Krieglstein K: TGF-beta is required for programmed cell death in interdigital webs of the developing mouse limb. Mech Dev. 113:111–120. 2002. View Article : Google Scholar : PubMed/NCBI

11 

Fogli M, Carlo-Stella C, Curti A, Ratta M, Tazzari PL, Ragazzi E, Colla S, Santucci AM, Tura S and Lemoli RM: Transforming growth factor beta3 inhibits chronic myelogenous leukemia hematopoiesis by inducing Fas-independent apoptosis. Exp Hematol. 28:775–783. 2000. View Article : Google Scholar : PubMed/NCBI

12 

Booth D and Potten CS: Protection against mucosal injury by growth factors and cytokines. J Natl Cancer Inst Monogr. 16–20. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Mylotte LA, Duffy AM, Murphy M, O'Brien T, Samali A, Barry F and Szegezdi E: Metabolic flexibility permits mesenchymal stem cell survival in an ischemic environment. Stem Cells. 26:1325–1336. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Wang DW, Fermor B, Gimble JM, Awad HA and Guilak F: Influence of oxygen on the proliferation and metabolism of adipose derived adult stem cells. J Cell Physiol. 204:184–191. 2005. View Article : Google Scholar : PubMed/NCBI

15 

D'Amelio M, Cavallucci V and Cecconi F: Neuronal caspase-3 signaling: Not only cell death. Cell Death Differ. 17:1104–1114. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Sharifi S, Barar J, Hejazi MS and Samadi N: Roles of the Bcl-2/Bax ratio, caspase-8 and 9 in resistance of breast cancer cells to paclitaxel. Asian Pac J Cancer Prev. 15:8617–8622. 2014. View Article : Google Scholar : PubMed/NCBI

17 

McIlwain DR, Berger T and Mak TW: Caspase functions in cell death and disease. Cold Spring Harb Perspect Biol. 5:a0086562013. View Article : Google Scholar : PubMed/NCBI

18 

Sousa FG, Matuo R, Soares DG, Escargueil AE, Henriques JA, Larsen AK and Saffi J: PARPs and the DNA damage response. Carcinogenesis. 33:1433–1440. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Yang LJ, Chen Y, He J, Yi S, Wen L, Zhao S and Cui GH: Effects of gambogic acid on the activation of caspase-3 and downregulation of SIRT1 in RPMI-8226 multiple myeloma cells via the accumulation of ROS. Oncol Lett. 3:1159–1165. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Yue Y, Zhang P, Liu D, Yang JF, Nie C and Yang D: Hypoxia preconditioning enhances the viability of ADSCs to increase the survival rate of ischemic skin flaps in rats. Aesthetic Plast Surg. 37:159–170. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Zhang J, Zhang X, Xie F, Zhang Z, van Dam H, Zhang L and Zhou F: The regulation of TGF-β/SMAD signaling by protein deubiquitination. Protein Cell. 5:503–517. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Bian L, Zhai DY, Tous E, Rai R, Mauck RL and Burdick JA: Enhanced MSC chondrogenesis following delivery of TGF-β3 from alginate microspheres within hyaluronic acid hydrogels in vitro and in vivo. Biomaterials. 32:6425–6434. 2011. View Article : Google Scholar : PubMed/NCBI

23 

He F, Xiong W, Wang Y, Li L, Liu C, Yamagami T, Taketo MM, Zhou C and Chen Y: Epithelial Wnt/β-catenin signaling regulates palatal shelf fusion through regulation of Tgfβ3 expression. Dev Biol. 350:511–519. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Dunker N, Schmitt K and Krieglstein K: TGF-beta is required for programmed cell death in interdigital webs of the developing mouse limb. Mech Dev. 113:111–120. 2002. View Article : Google Scholar : PubMed/NCBI

25 

Wu BX, Li A, Lei L, Kaneko S, Wallace C, Li X and Li Z: Glycoprotein A repetitions predominant (GARP) positively regulates transforming growth factor (TGF) β3 and is essential for mouse palatogenesis. J Biol Chem. 292:18091–18097. 2017. View Article : Google Scholar : PubMed/NCBI

26 

Haleem AM and Chu CR: Advances in tissue engineering techniques for articular cartilage repair. Oper Tech Orthop. 20:76–89. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Deng J, Han Y, Yan C, Tian X, Tao J, Kang J and Li S: Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt. Apoptosis. 15:463–473. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Zhu W, Chen J, Cong X, Hu S and Chen X: Hypoxia and serum deprivation-induced apoptosis in mesenchymal stem cells. Stem Cells. 24:416–425. 2006. View Article : Google Scholar : PubMed/NCBI

29 

Hou M, Liu J, Liu F, Liu K and Yu B: C1q tumor necrosis factor-related protein-3 protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis through the phosphoinositide 3-kinase/Akt pathway. Int J Mol Med. 33:97–1044. 2014. View Article : Google Scholar : PubMed/NCBI

30 

Deschepper M, Oudina K, David B, Myrtil V, Collet C, Bensidhoum M, Logeart-Avramoglou D and Petite H: Survival and function of mesenchymal stem cells (MSCs) depend on glucose to overcome exposure to long-term, severe and continuous hypoxia. J Cell Mol Med. 15:1505–1514. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wu F, Ye H, Lin J, Xu Y, Zhang Z, Xiong H, Laing M, Zhen Y and Chen S: TGF-β3 reduces apoptosis in ischemia-induced adipose-derived stem cells by enhancing DNA repair. Exp Ther Med 15: 4400-4408, 2018.
APA
Wu, F., Ye, H., Lin, J., Xu, Y., Zhang, Z., Xiong, H. ... Chen, S. (2018). TGF-β3 reduces apoptosis in ischemia-induced adipose-derived stem cells by enhancing DNA repair. Experimental and Therapeutic Medicine, 15, 4400-4408. https://doi.org/10.3892/etm.2018.5980
MLA
Wu, F., Ye, H., Lin, J., Xu, Y., Zhang, Z., Xiong, H., Laing, M., Zhen, Y., Chen, S."TGF-β3 reduces apoptosis in ischemia-induced adipose-derived stem cells by enhancing DNA repair". Experimental and Therapeutic Medicine 15.5 (2018): 4400-4408.
Chicago
Wu, F., Ye, H., Lin, J., Xu, Y., Zhang, Z., Xiong, H., Laing, M., Zhen, Y., Chen, S."TGF-β3 reduces apoptosis in ischemia-induced adipose-derived stem cells by enhancing DNA repair". Experimental and Therapeutic Medicine 15, no. 5 (2018): 4400-4408. https://doi.org/10.3892/etm.2018.5980
Copy and paste a formatted citation
x
Spandidos Publications style
Wu F, Ye H, Lin J, Xu Y, Zhang Z, Xiong H, Laing M, Zhen Y and Chen S: TGF-β3 reduces apoptosis in ischemia-induced adipose-derived stem cells by enhancing DNA repair. Exp Ther Med 15: 4400-4408, 2018.
APA
Wu, F., Ye, H., Lin, J., Xu, Y., Zhang, Z., Xiong, H. ... Chen, S. (2018). TGF-β3 reduces apoptosis in ischemia-induced adipose-derived stem cells by enhancing DNA repair. Experimental and Therapeutic Medicine, 15, 4400-4408. https://doi.org/10.3892/etm.2018.5980
MLA
Wu, F., Ye, H., Lin, J., Xu, Y., Zhang, Z., Xiong, H., Laing, M., Zhen, Y., Chen, S."TGF-β3 reduces apoptosis in ischemia-induced adipose-derived stem cells by enhancing DNA repair". Experimental and Therapeutic Medicine 15.5 (2018): 4400-4408.
Chicago
Wu, F., Ye, H., Lin, J., Xu, Y., Zhang, Z., Xiong, H., Laing, M., Zhen, Y., Chen, S."TGF-β3 reduces apoptosis in ischemia-induced adipose-derived stem cells by enhancing DNA repair". Experimental and Therapeutic Medicine 15, no. 5 (2018): 4400-4408. https://doi.org/10.3892/etm.2018.5980
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