Open Access

Relationship between HOX gene and pediatric congenital clubfoot

  • Authors:
    • Yuanhui Wang
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  • Published online on: March 30, 2018     https://doi.org/10.3892/etm.2018.6013
  • Pages: 4861-4865
  • Copyright: © Wang . This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The relationship between transcription factor homeobox gene (HOX gene) and pediatric congenital clubfoot (CCF) was studied. The CCF group comprised 35 cases of children, and the control group compised 34 cases of children without congenital malformation. The levels of inducible nitric oxide synthase (iNOS) and nitric oxide (NO) in the serum of the control and CCF groups were measured using iNOS and NO kits. Interleukin-1β (IL-1β), IL-6 and tumor necrosis factor-α (TNF-α) related to inflammation in the tissues of both groups were detected by reverse transcription‑polymerase chain reaction (RT-PCR). Fatty acid synthase (Fas), Fas ligand (FasL) and Bcl-2‑associated X (Bax) related to apoptosis as well as the expression of HOX mRNA, the expression of HOX in the control and CCF groups was detected by western blot analysis, and the differential expression of HOX in the control and CCF groups was statistically analyzed. Results of the kit detection showed that the expression of iNOS and NO in the CCF group were significantly higher than those in the control group, indicating that severe oxidative damage occurred in the CCF group. The results of detecting inflammatory factors and apoptosis by RT-PCR showed that the expression of IL-1β, IL-6, TNF-α, Fas, FasL and Bax mRNA in the CCF group was significantly higher than that in the control group, indicating pathogenesis of CCF was related to inflammation and apoptosis. RT-PCR and western blot analysis revealed HOX was highly expressed in the tissues of CCF, and the expression quantity was significantly stronger than that in the control group. The result of analysis of variance showed that the expression differences of HOX in normal and CCF tissues were statistically significant (P<0.01). Abnormal expression of HOX was closely related to the occurrence and development of CCF, indicating that HOX has important research value in CCF and this functional mechanism is related to oxidative damage, inflammation and apoptosis. Expression of HOX therefore shows promise as an indicator of CCF diagnosis and treatment.
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June-2018
Volume 15 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wang Y: Relationship between HOX gene and pediatric congenital clubfoot. Exp Ther Med 15: 4861-4865, 2018
APA
Wang, Y. (2018). Relationship between HOX gene and pediatric congenital clubfoot. Experimental and Therapeutic Medicine, 15, 4861-4865. https://doi.org/10.3892/etm.2018.6013
MLA
Wang, Y."Relationship between HOX gene and pediatric congenital clubfoot". Experimental and Therapeutic Medicine 15.6 (2018): 4861-4865.
Chicago
Wang, Y."Relationship between HOX gene and pediatric congenital clubfoot". Experimental and Therapeutic Medicine 15, no. 6 (2018): 4861-4865. https://doi.org/10.3892/etm.2018.6013