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Treatment of Saos‑2 osteosarcoma cells with diallyl trisulfide is associated with an increase in calreticulin expression

  • Authors:
    • Wen‑Peng Xie
    • Yue Zhang
    • Yong‑Kui Zhang
    • Gang Li
    • Jian Xin
    • Rong‑Xiu Bi
    • Chuan‑Jie Li
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, First Clinical Medical College, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250355, P.R. China, Department of Orthopedics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250014, P.R. China, Department of Orthopedics, Laiwu Central Hospital of Xinwen Mining Group, Laiwu, Shandong 271103, P.R. China
    Copyright: © Xie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4737-4742
    |
    Published online on: April 5, 2018
       https://doi.org/10.3892/etm.2018.6037
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Abstract

Diallyl trisulfide (DATS) is a natural organic sulfur compound that may be isolated from garlic and has strong anticancer activity. DATS has been demonstrated to upregulate the expression of calreticulin (CRT) in various types of human cancers, which is associated with the prognosis of cancer and its response to therapy. However, whether DATS has the same effect on human osteosarcoma cells is not known. Therefore, in the present study, Saos‑2 human osteosarcoma cells were cultured with different concentrations of DATS (0, 25, 50 and 100 µmol/l) for 24 h, or with 50 µmol/l DATS for different time periods (0, 12, 24 and 36 h). Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), western blotting and immunofluorescent staining were used to detect CRT mRNA and protein in the Saos‑2 cells. Exposure to DATS changed the morphology and inhibited the growth of the Saos‑2 cells, and its effects appeared to be concentration‑ and exposure time‑dependent. The optimum concentration and exposure time of DATS were 50 µmol/l and 24 h, respectively. The levels of CRT mRNA and protein in the Saos‑2 cells were significantly upregulated following exposure to DATS. The upregulation of CRT expression by DATS may be a mechanism underlying the ability of DATS to inhibit the growth of human osteosarcoma Saos‑2 cells.
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1 

Chen N, Zhang R, Konishi T and Wang J: Upregulation of NRF2 through autophagy/ERK 1/2 ameliorates ionizing radiation induced cell death of human osteosarcoma U-2 OS. Mutat Res. 813:10–17. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Bielack SS, Hecker-Nolting S, Blattmann C and Kager L: Advances in the management of osteosarcoma. F1000 Res. 5:27672016. View Article : Google Scholar

3 

Friebele JC, Peck J, Pan X, Abdel-Rasoul M and Mayerson JL: Osteosarcoma: A meta-analysis and review of the literature. Am J Orthop (Belle Mead NJ). 44:547–553. 2015.PubMed/NCBI

4 

Liu K, Sun X, Zhang Y, Liu L and Yuan Q: MiR-598: A tumor suppressor with biomarker significance in osteosarcoma. Life Sci. 188:141–148. 2017. View Article : Google Scholar : PubMed/NCBI

5 

Taran SJ, Taran R and Malipatil NB: Pediatric osteosarcoma: An updated review. Indian J Med Paediatr Oncol. 38:33–43. 2017. View Article : Google Scholar : PubMed/NCBI

6 

Chen L, Pei H, Lu SJ, Liu ZJ, Yan L, Zhao XM, Hu B and Lu HG: SPOP suppresses osteosarcoma invasion via PI3K/AKT/NF-κB signaling pathway. Eur Rev Med Pharmacol Sci. 22:609–615. 2018.PubMed/NCBI

7 

He X, Gao Z, Xu H, Zhang Z and Fu P: A meta-analysis of randomized control trials of surgical methods with osteosarcoma outcomes. J Orthop Surg Res. 12:52017. View Article : Google Scholar : PubMed/NCBI

8 

Isakoff MS, Bielack SS, Meltzer P and Gorlick R: Osteosarcoma: Current treatment and a collaborative pathway to success. J Clin Oncol. 33:3029–3035. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Anderson ME: Update on survival in osteosarcoma. Orthop Clin North Am. 47:283–292. 2016. View Article : Google Scholar : PubMed/NCBI

10 

Sun J, Mu H, Dai K and Yi L: Calreticulin: A potential anti-cancer therapeutic target. Pharmazie. 72:503–510. 2017.PubMed/NCBI

11 

Harada K, Takenawa T, Ferdous T, Kuramitsu Y and Ueyama Y: Calreticulin is a novel independent prognostic factor for oral squamous cell carcinoma. Oncol Lett. 13:4857–4862. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Fucikova J, Truxova I, Hensler M, Becht E, Kasikova L, Moserova I, Vosahlikova S, Klouckova J, Church SE, Cremer I, et al: Calreticulin exposure by malignant blasts correlates with robust anticancer immunity and improved clinical outcome in AML patients. Blood. 128:3113–3124. 2016.PubMed/NCBI

13 

Obakan-Yerlikaya P, Arisan ED, Coker-Gurkan A, Adacan K, Ozbey U, Somuncu B, Baran D and Palavan-Unsal N: Calreticulin is a fine tuning molecule in epibrassinolide-induced apoptosis through activating endoplasmic reticulum stress in colon cancer cells. Mol Carcinog. 56:1603–1619. 2017. View Article : Google Scholar : PubMed/NCBI

14 

Matsukuma S, Yoshimura K, Ueno T, Oga A, Inoue M, Watanabe Y, Kuramasu A, Fuse M, Tsunedomi R, Nagaoka S, et al: Calreticulin is highly expressed in pancreatic cancer stem-like cells. Cancer Sci. 107:1599–1609. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Zamanian M, Hamadneh Qader LA, Veerakumarasivam A, Rahman Abdul S, Shohaimi S and Rosli R: Calreticulin mediates an invasive breast cancer phenotype through the transcriptional dysregulation of p53 and MAPK pathways. Cancer Cell Int. 16:562016. View Article : Google Scholar : PubMed/NCBI

16 

Fucikova J, Becht E, Iribarren K, Goc J, Remark R, Damotte D, Alifano M, Devi P, Biton J, Germain C, et al: Calreticulin expression in human non-small cell lung cancers correlates with increased accumulation of antitumor immune cells and favorable prognosis. Cancer Res. 76:1746–1756. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Feng R, Ye J, Zhou C, Qi L, Fu Z, Yan B, Liang Z, Li R and Zhai W: Calreticulin down-regulation inhibits the cell growth, invasion and cell cycle progression of human hepatocellular carcinoma cells. Diagn Pathol. 10:1492015. View Article : Google Scholar : PubMed/NCBI

18 

Yang MS, Wang HS, Wang BS, Li WH, Pang ZF, Zou BK, Zhang X, Shi XT, Mu DB, Zhang DX, et al: A comparative proteomic study identified calreticulin and prohibitin up-regulated in adrenocortical carcinomas. Diagn Pathol. 8:582013. View Article : Google Scholar : PubMed/NCBI

19 

Chen CN, Chang CC, Su TE, Hsu WM, Jeng YM, Ho MC, Hsieh FJ, Lee PH, Kuo ML, Lee H and Chang KJ: Identification of calreticulin as a prognosis marker and angiogenic regulator in human gastric cancer. Ann Surg Oncol. 16:524–533. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Zhang XH, Zhang Y, Xie WP, Sun DS, Zhang YK, Hao YK and Tan GQ: Expression and significance of calreticulin in human osteosarcoma. Cancer Biomark. 18:405–411. 2017. View Article : Google Scholar : PubMed/NCBI

21 

Mikaili P, Maadirad S, Moloudizargari M, Aghajanshakeri S and Sarahroodi S: Therapeutic uses and pharmacological properties of garlic, shallot, and their biologically active compounds. Iran J Basic Med Sci. 16:1031–1048. 2013.PubMed/NCBI

22 

Horn N, Miller G, Ajuwon KM and Adeola O: Garlic diallyl disulfide and diallyl trisulfide mitigates effects of pro-oxidant induced cellular stress and has immune modulatory function in LPS-stimulated porcine epithelial cells. J Anim Sci. 95:4045–4051. 2017. View Article : Google Scholar : PubMed/NCBI

23 

Antony ML and Singh SV: Molecular mechanisms and targets of cancer chemoprevention by garlic-derived bioactive compound diallyl trisulfide. Indian J Exp Biol. 49:805–816. 2011.PubMed/NCBI

24 

Seki T, Hosono T, Hosono-Fukao T, Inada K, Tanaka R, Ogihara J and Ariga T: Anticancer effects of diallyl trisulfide derived from garlic. Asia Pac J Clin Nutr. 17 Suppl 1:S249–S252. 2008.

25 

Zhang YK, Zhang XH, Li JM, Sun DS, Yang Q and Diao DM: A proteomic study on a human osteosarcoma cell line Saos-2 treated with diallyl trisulfide. Anticancer Drugs. 20:702–712. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

27 

Zhang YK, Li JM, Wang DL and Chen YQ: Effects of diallyl trisulfide on cell cycle and apoptosis of human osteosarcoma cell line Saos-2. Tumor. 33:214–222. 2013.(In Chinese).

28 

Yuan G, Chen J, Wu D and Gao C: Neoadjuvant chemotherapy combined with limb salvage surgery in patients with limb osteosarcoma of Enneking stage II: A retrospective study. Onco Targets Ther. 10:2745–2750. 2017. View Article : Google Scholar : PubMed/NCBI

29 

Rosen G, Marcove RC, Caparros B, Nirenberg A, Kosloff C and Huvos AG: Primary osteogenic sarcoma: The rationale for preoperative chemotherapy and delayed surgery. Cancer. 43:2163–2177. 1979. View Article : Google Scholar : PubMed/NCBI

30 

Masood S: Neoadjuvant chemotherapy in breast cancers. Womens Health (Lond). 12:480–491. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Borkowska A, Knap N and Antosiewicz J: Diallyl trisulfide is more cytotoxic to prostate cancer cells PC-3 than to noncancerous epithelial cell line PNT1A: A possible role of p66Shc signaling axis. Nutr Cancer. 65:711–717. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Pan Y, Lin S, Xing R, Zhu M, Lin B, Cui J, Li W, Gao J, Shen L, Zhao Y, et al: Epigenetic upregulation of metallothionein 2A by diallyl trisulfide enhances chemosensitivity of human gastric cancer cells to docetaxel through attenuating NF-κB activation. Antioxid Redox Signal. 24:839–854. 2016. View Article : Google Scholar : PubMed/NCBI

33 

Li W, Tian H, Li L, Li S, Yue W, Chen Z, Qi L, Hu W, Zhu Y, Hao B, et al: Diallyl trisulfide induces apoptosis and inhibits proliferation of A549 cells in vitro and in vivo. Acta Biochim Biophys Sin (Shanghai). 44:577–583. 2012. View Article : Google Scholar : PubMed/NCBI

34 

Kim SH, Kaschula CH, Priedigkeit N, Lee AV and Singh SV: Forkhead box Q1 is a novel target of breast cancer stem cell inhibition by diallyl trisulfide. J Biol Chem. 291:13495–13508. 2016. View Article : Google Scholar : PubMed/NCBI

35 

Lai KC, Hsu SC, Yang JS, Yu CC, Lein JC and Chung JG: Diallyl trisulfide inhibits migration, invasion and angiogenesis of human colon cancer HT-29 cells and umbilical vein endothelial cells, and suppresses murine xenograft tumour growth. J Cell Mol Med. 19:474–484. 2015. View Article : Google Scholar : PubMed/NCBI

36 

Oommen S, Anto RJ, Srinivas G and Karunagaran D: Allicin (from garlic) induces caspase-mediated apoptosis in cancer cells. Eur J Pharmacol. 485:97–103. 2004. View Article : Google Scholar : PubMed/NCBI

37 

Xu L, Yu J, Zhai D, Zhang D, Shen W, Bai L, Cai Z and Yu C: Role of JNK activation and mitochondrial bax translocation in allicin-induced apoptosis in human ovarian cancer SKOV3 cells. Evid Based Complement Alternat Med. 2014:3786842014. View Article : Google Scholar : PubMed/NCBI

38 

Li C, Lun W, Zhao X, Lei S, Guo Y, Ma J and Zhi F: Allicin alleviates inflammation of trinitrobenzenesulfonic acid-induced rats and suppresses P38 and JNK pathways in Caco-2 cells. Mediators Inflamm. 2015:4346922015. View Article : Google Scholar : PubMed/NCBI

39 

Tufi R, Panaretakis T, Bianchi K, Criollo A, Fazi B, Di Sano F, Tesniere A, Kepp O, Paterlini-Brechot P, Zitvogel L, et al: Reduction of endoplasmic reticulum Ca2+ levels favors plasma membrane surface exposure of calreticulin. Cell Death Differ. 15:274–282. 2008. View Article : Google Scholar : PubMed/NCBI

40 

Chao MP, Jaiswal S, Weissman-Tsukamoto R, Alizadeh AA, Gentles AJ, Volkmer J, Weiskopf K, Willingham SB, Raveh T, Park CY, et al: Calreticulin is the dominant pro-phagocytic signal on multiple human cancers and is counterbalanced by CD47. Sci Transl Med. 2:63–94. 2010. View Article : Google Scholar

41 

No authors listed: Calreticulin inhibits commitment to adipocyte differentiation. J Cell Biol. 208:249–250. 2015. View Article : Google Scholar : PubMed/NCBI

42 

Martín N, Bardisa L, Pantoja C, Barra E, Demetrio C, Valenzuela J, Barrios M and Sepúlveda MJ: Involvement of calcium in the cardiac depressant actions of a garlic dialysate. J Ethnopharmacol. 55:113–118. 1997. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xie WP, Zhang Y, Zhang YK, Li G, Xin J, Bi RX and Li CJ: Treatment of Saos‑2 osteosarcoma cells with diallyl trisulfide is associated with an increase in calreticulin expression. Exp Ther Med 15: 4737-4742, 2018.
APA
Xie, W., Zhang, Y., Zhang, Y., Li, G., Xin, J., Bi, R., & Li, C. (2018). Treatment of Saos‑2 osteosarcoma cells with diallyl trisulfide is associated with an increase in calreticulin expression. Experimental and Therapeutic Medicine, 15, 4737-4742. https://doi.org/10.3892/etm.2018.6037
MLA
Xie, W., Zhang, Y., Zhang, Y., Li, G., Xin, J., Bi, R., Li, C."Treatment of Saos‑2 osteosarcoma cells with diallyl trisulfide is associated with an increase in calreticulin expression". Experimental and Therapeutic Medicine 15.6 (2018): 4737-4742.
Chicago
Xie, W., Zhang, Y., Zhang, Y., Li, G., Xin, J., Bi, R., Li, C."Treatment of Saos‑2 osteosarcoma cells with diallyl trisulfide is associated with an increase in calreticulin expression". Experimental and Therapeutic Medicine 15, no. 6 (2018): 4737-4742. https://doi.org/10.3892/etm.2018.6037
Copy and paste a formatted citation
x
Spandidos Publications style
Xie WP, Zhang Y, Zhang YK, Li G, Xin J, Bi RX and Li CJ: Treatment of Saos‑2 osteosarcoma cells with diallyl trisulfide is associated with an increase in calreticulin expression. Exp Ther Med 15: 4737-4742, 2018.
APA
Xie, W., Zhang, Y., Zhang, Y., Li, G., Xin, J., Bi, R., & Li, C. (2018). Treatment of Saos‑2 osteosarcoma cells with diallyl trisulfide is associated with an increase in calreticulin expression. Experimental and Therapeutic Medicine, 15, 4737-4742. https://doi.org/10.3892/etm.2018.6037
MLA
Xie, W., Zhang, Y., Zhang, Y., Li, G., Xin, J., Bi, R., Li, C."Treatment of Saos‑2 osteosarcoma cells with diallyl trisulfide is associated with an increase in calreticulin expression". Experimental and Therapeutic Medicine 15.6 (2018): 4737-4742.
Chicago
Xie, W., Zhang, Y., Zhang, Y., Li, G., Xin, J., Bi, R., Li, C."Treatment of Saos‑2 osteosarcoma cells with diallyl trisulfide is associated with an increase in calreticulin expression". Experimental and Therapeutic Medicine 15, no. 6 (2018): 4737-4742. https://doi.org/10.3892/etm.2018.6037
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