MicroRNA‑183 suppresses the vitality, invasion and migration of human osteosarcoma cells by targeting metastasis‑associated protein 110.3892/etm.2021.10947

Sun,Xiaoya; Xu,Yan; Zhang,Shanfeng; Li,Xinjie; Wang,Yadong; Zhang,Yan; Zhao,Xuefeng; Li,Yuebai; Wang,Yisheng

doi:10.3892/etm.2018.6068

MicroRNA‑183 suppresses the vitality, invasion and migration of human osteosarcoma cells by targeting metastasis‑associated protein 110.3892/etm.2021.10947

Authors:
- Xiaoya Sun
- Yan Xu
- Shanfeng Zhang
- Xinjie Li
- Yadong Wang
- Yan Zhang
- Xuefeng Zhao
- Yuebai Li
- Yisheng Wang
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Affiliations: Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan 450001, P.R. China, Department of Orthopedic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
Published online on: April 13, 2018 https://doi.org/10.3892/etm.2018.6068
Pages: 5058-5064

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Abstract

The aim of the present study was to investigate the effects of microRNA (miR)‑183 on vitality, invasion, metastasis and apoptosis in osteosarcoma (OS) cells, mediated by its binding to metastasis‑associated protein 1 (MTA1). A dual luciferase reporter assay was performed to determine whether MTA1 was a direct target of miR‑183. Cell Counting Kit‑8, Transwell, scratch‑wound healing, fluorescence‑activated cell sorting andterminal deoxynucleotidyl transferase dUTP nick end labeling assays were also performed to investigate the effects of miR‑183 expression on the proliferation, invasion, migration and apoptosis of MG63 cells. It was demonstrated that that MTA1 expression levels were significantly higher in OS tissues and MG63 cells compared with corresponding adjacent noncancerous tissues and normal cells, respectively, while miR‑183 expression levels were significantly lower (both P<0.05). Furthermore, miR‑183 overexpression downregulated MTA1 levels and inhibited cell proliferation (P<0.05), migration (P<0.05) and invasion (P<0.01), as well as promoting apoptosis (P<0.01) by binding to the 3'‑untranslated region of MTA1. These results indicate that miR‑183 inhibits the vitality, invasion, migration and apoptosis of the OS cell line MG63 by targeting MTA1. These findings may contribute to the development of novel clinical therapeutic approaches for the treatment of OS.

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