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Article

Expression and role of VEGFA and miR‑381 in portal vein tumor thrombi in patients with hepatocellular carcinoma

  • Authors:
    • Jing Wang
    • Shuzhi Wu
    • Tianren Huang
  • View Affiliations / Copyright

    Affiliations: Experimental Department, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China, Institute for Viral Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, Shandong 250014, P.R. China
  • Pages: 5450-5456
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    Published online on: May 3, 2018
       https://doi.org/10.3892/etm.2018.6129
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Abstract

The aim of the present study was to examine the expression and role of vascular endothelial growth factor A (VEGFA) and microRNA (miRNA or miR)‑381 in tumor thrombi from patients with hepatocellular carcinoma and portal vein tumor thrombus (PVTT). Tumor thrombi and adjacent paired tissues were collected from 39 patients with hepatocellular carcinoma with PVTT. VEGFA expression levels were assessed using reverse transcription‑quantitative polymerase chain reaction and western blotting. miRNAs that may regulate VEGFA expression were predicted using bioinformatics analysis and confirmed via a dual luciferase reporter assay. The effects of VEGFA and its upstream miRNA on proliferation of the proliferation of EAhy926 human venous endothelial cells were analyzed using an MTT assay. Compared with the paired adjacent tissues, VEGFA was significantly upregulated at both the mRNA and protein level in tumor thrombi (P<0.05). VEGFA was predicted to be a target of miR‑381 and this was confirmed experimentally. miR‑381 expression was significantly downregulated in tumor thrombi from patients with PVTT compared with paired adjacent tissues (P<0.05). In addition, transfection with antagomirs against miR‑381 or short interfering RNA against VEGFA significantly inhibited EAhy926 cell proliferation (P<0.05). In conclusion, the results of the present study indicate that VEGFA is upregulated in tumor thrombi whereas miR‑381 is downregulated. VEGFA is regulated by miR‑381 and both may be associated with the development of PVTT.
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Copy and paste a formatted citation
Spandidos Publications style
Wang J, Wu S and Huang T: Expression and role of VEGFA and miR‑381 in portal vein tumor thrombi in patients with hepatocellular carcinoma. Exp Ther Med 15: 5450-5456, 2018.
APA
Wang, J., Wu, S., & Huang, T. (2018). Expression and role of VEGFA and miR‑381 in portal vein tumor thrombi in patients with hepatocellular carcinoma. Experimental and Therapeutic Medicine, 15, 5450-5456. https://doi.org/10.3892/etm.2018.6129
MLA
Wang, J., Wu, S., Huang, T."Expression and role of VEGFA and miR‑381 in portal vein tumor thrombi in patients with hepatocellular carcinoma". Experimental and Therapeutic Medicine 15.6 (2018): 5450-5456.
Chicago
Wang, J., Wu, S., Huang, T."Expression and role of VEGFA and miR‑381 in portal vein tumor thrombi in patients with hepatocellular carcinoma". Experimental and Therapeutic Medicine 15, no. 6 (2018): 5450-5456. https://doi.org/10.3892/etm.2018.6129
Copy and paste a formatted citation
x
Spandidos Publications style
Wang J, Wu S and Huang T: Expression and role of VEGFA and miR‑381 in portal vein tumor thrombi in patients with hepatocellular carcinoma. Exp Ther Med 15: 5450-5456, 2018.
APA
Wang, J., Wu, S., & Huang, T. (2018). Expression and role of VEGFA and miR‑381 in portal vein tumor thrombi in patients with hepatocellular carcinoma. Experimental and Therapeutic Medicine, 15, 5450-5456. https://doi.org/10.3892/etm.2018.6129
MLA
Wang, J., Wu, S., Huang, T."Expression and role of VEGFA and miR‑381 in portal vein tumor thrombi in patients with hepatocellular carcinoma". Experimental and Therapeutic Medicine 15.6 (2018): 5450-5456.
Chicago
Wang, J., Wu, S., Huang, T."Expression and role of VEGFA and miR‑381 in portal vein tumor thrombi in patients with hepatocellular carcinoma". Experimental and Therapeutic Medicine 15, no. 6 (2018): 5450-5456. https://doi.org/10.3892/etm.2018.6129
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