Influence of vitamin D deficiency on T cell subsets and related indices during spinal tuberculosis

Zhou,Shao-Huai; Wang,Xin; Fan,Ming-Yu; Li,Hong-Liang; Bian,Feng; Huang,Tao; Fang,Hong-Yu

doi:10.3892/etm.2018.6203

August-2018 Volume 16 Issue 2

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August-2018 Volume 16 Issue 2

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Article Open Access

Influence of vitamin D deficiency on T cell subsets and related indices during spinal tuberculosis

Authors:
- Shao-Huai Zhou
- Xin Wang
- Ming-Yu Fan
- Hong-Liang Li
- Feng Bian
- Tao Huang
- Hong-Yu Fang
View Affiliations / Copyright

Affiliations: Department of Orthopaedics, Wuhan City Third Hospital, Wuhan, Hubei 430060, P.R. China

Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 718-722
|
Published online on: May 22, 2018

https://doi.org/10.3892/etm.2018.6203
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Abstract

The present study investigated the effects of vitamin D deficiency on T cell subsets in patients with spinal tuberculosis. In addition, the influence of vitamin D deficiency was investigated on the expression of cytokines IL-1β, IL-6 and TNF-α in intervertebral disc lesions of patients. One hundred and seventeen patients with spinal tuberculosis who received operative treatment in the Department of Orthopedics in Wuhan City Third Hospital from March 2012 to March 2015 were collected. The patients were divided depending upon vitamin D content into the control group (64 cases, vitamin D content <25 nmol/l) and experimental group (53 cases, vitamin D content >50 nmol/l). Immunofluorescence method was applied to determine the content of T cell subsets in both groups of patients. Intervertebral disc lesion tissues of two groups of patients were obtained during surgery then treated with HE staining and immunohistochemical staining. The values of average optical density obtained under light microscope were observed as the expression quantities of IL-1β, IL-6 and TNF-α, to explore the relationship between vitamin D and the expression of cytokines. When vitamin D is lacking, the expression of T lymphocyte subsets in patients with spinal tuberculosis significantly decreased. Compared with experimental group, the difference was statistically significant (P<0.05). Further, the expression of cytokines IL-1β, IL-6 and TNF-α in intervertebral disc lesion tissues of patients with spinal tuberculosis were significantly higher than those of patients with spinal tuberculosis whose vitamin D content was normal (P<0.05). In the control group, vitamin D content was negatively correlated with the expression of IL-1β, IL-6 and TNF-α. The expression of T lymphocyte subsets in patients with vitamin D deficiency was significantly reduced, and the immune function decreased. The expression of IL-1β, IL-6 and TNF-α in lesions were significantly higher than those of patients with normal vitamin D content. In addition, the lower the content of vitamin D was, the more active the expression of inflammatory factors were, which was not conducive to the recovery of tuberculosis lesions.

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1	Garg RK and Somvanshi DS: Spinal tuberculosis: A review. J Spinal Cord Med. 34:440–454. 2011. View Article : Google Scholar : PubMed/NCBI
2	Zheng C, Li P and Kan W: Video-assisted thoracoscopic anterior surgery combined posterior instrumentation for children with spinal tuberculosis. Eur J Pediatr Surg. 24:83–87. 2014. View Article : Google Scholar : PubMed/NCBI
3	Tuli SM: General principles of osteoarticular tuberculosis. Clin Orthop Relat Res. 398:11–19. 2002. View Article : Google Scholar
4	Hewison M: Vitamin D and immune function: An overview. Proc Nutr Soc. 71:50–61. 2012. View Article : Google Scholar : PubMed/NCBI
5	Arya SC and Agarwal N: Vitamin D deficiency in adult tuberculosis patients. Int J Tuberc Lung Dis. 15:1133–1134. 2011. View Article : Google Scholar : PubMed/NCBI
6	Mathema B, Kurepina NE, Bifani PJ and Kreiswirth BN: Molecular epidemiology of tuberculosis: Current insights. Clin Microbiol Rev. 19:658–685. 2006. View Article : Google Scholar : PubMed/NCBI
7	Martineau AR, Timms PM, Bothamley GH, Hanifa Y, Islam K, Claxton AP, Packe GE, Moore-Gillon JC, Darmalingam M, Davidson RN, et al: High-dose vitamin D(3) during intensive-phase antimicrobial treatment of pulmonary tuberculosis: A double-blind randomised controlled trial. Lancet. 377:242–250. 2011. View Article : Google Scholar : PubMed/NCBI
8	Kota SK, Jammula S, Kota SK, Tripathy PR, Panda S and Modi KD: Effect of vitamin D supplementation in type 2 diabetes patients with pulmonary tuberculosis. Diabetes Metab Syndr. 5:85–89. 2011. View Article : Google Scholar : PubMed/NCBI
9	Hughes DA and Norton R: Vitamin D and respiratory health. Clin Exp Immunol. 158:20–25. 2009. View Article : Google Scholar : PubMed/NCBI
10	Khoo AL, Chai LY, Koenen HJ, Oosting M, Steinmeyer A, Zuegel U, Joosten I, Netea MG and van der Ven AJ: Vitamin D(3) down-regulates proinflammatory cytokine response to Mycobacterium tuberculosis through pattern recognition receptors while inducing protective cathelicidin production. Cytokine. 55:294–300. 2011. View Article : Google Scholar : PubMed/NCBI
11	Warmelink I, van Altena R, ten Hacken N, van der Werf T and van der Veer E: Nutritional status and vitamin D3 during antimicrobial treatment. Lancet. 377:1407–1408, author reply 1408. 2011. View Article : Google Scholar : PubMed/NCBI
12	Bikle DD: Vitamin D regulation of immune function. Vitam Horm. 86:1–21. 2011. View Article : Google Scholar : PubMed/NCBI
13	Luong K and Nguyen LT: Impact of vitamin D in the treatment of tuberculosis. Am J Med Sci. 341:493–498. 2011. View Article : Google Scholar : PubMed/NCBI
14	Taylor CE and Camargo CA Jr: Impact of micronutrients on respiratory infections. Nutr Rev. 69:259–269. 2011. View Article : Google Scholar : PubMed/NCBI
15	Raja A: Immunology of tuberculosis. Indian J Med Res. 120:213–232. 2004.PubMed/NCBI
16	Takahasi H, Suguro T, Okazima Y, Motegi M, Okada Y and Kakiuchi T: Inflammatory cytokines in the herniated dise of the lumber. Spine (Phila Pa 1976). 21:218–224. 1996. View Article : Google Scholar : PubMed/NCBI
17	Weitzmann MN, Cenci S, Rifas L, Brown C and Pacifici R: Interleukin-7 stimulates osteoclast formation by up-regulating the T-cell production of soluble osteoclastogenic cytokines. Blood. 96:1873–1878. 2000.PubMed/NCBI
18	Zganiacz A, Santosuosso M, Wang J, Yang T, Chen L, Anzulovic M, Alexander S, Gicquel B, Wan Y, Bramson J, et al: TNF-alpha is a critical negative regulator of type 1 immune activation during intracellular bacterial infection. J Clin Inves. 113:401–413. 2004. View Article : Google Scholar
19	Akyol S, Eraslan BS, Etyemez H, Tanriverdi T and Hanci M: Catabolic cytokine expressions in patients with degenerative disc disease. Turk Neurosurg. 20:492–499. 2010.PubMed/NCBI
20	Bai X, Wilson SE, Chmura K, Feldman NE and Chan ED: Morphometric analysis of Th(1) and Th(2) cytokine expression in human pulmonary tuberculosis. Tuberculosis (Edinb). 84:375–385. 2004. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Influence of vitamin D deficiency on T cell subsets and related indices during spinal tuberculosis

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