Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
August-2018 Volume 16 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2018 Volume 16 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Identification of potential key genes associated with severe pneumonia using mRNA‑seq

  • Authors:
    • Cong Feng
    • He Huang
    • Sai Huang
    • Yong‑Zhi Zhai
    • Jing Dong
    • Li Chen
    • Zhi Huang
    • Xuan Zhou
    • Bei Li
    • Li‑Li Wang
    • Wei Chen
    • Fa‑Qin Lv
    • Tan‑Shi Li
  • View Affiliations / Copyright

    Affiliations: Department of Emergency, Chinese PLA General Hospital, Beijing 100853, P.R. China, Department of Critical Care Medicine, General Hospital of Jinan Command, Jinan, Shandong 250031, P.R. China, Department of Electrical and Computer Engineering, Purdue University, Indianapolis, IN 46202, USA, Department of Ultrasound, Chinese PLA General Hospital, Beijing 100853, P.R. China
    Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 758-766
    |
    Published online on: June 7, 2018
       https://doi.org/10.3892/etm.2018.6262
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

This study aimed to identify the potential key genes associated with severe pneumonia using mRNA‑seq. Nine peripheral blood samples from patients with severe pneumonia alone (SP group, n=3) and severe pneumonia accompanied with chronic obstructive pulmonary disease (COPD; CSP group, n=3), as well as volunteers without pneumonia (control group, n=3) underwent mRNA‑seq. Based on the sequencing data, differentially expressed genes (DEGs) were identified by Limma package. Following the pathway enrichment analysis of DEGs, the genes that were differentially expressed in the SP and CSP groups were selected for pathway enrichment analysis and coexpression analysis. In addition, potential genes related to pneumonia were identified based on the information in the Comparative Toxicogenomics Database. In total, 645 and 528 DEGs were identified in the SP and CSP groups, respectively, compared with the normal controls. Among these DEGs, 88 upregulated genes and 80 downregulated genes were common between the two groups. The functions of the common DEGs were similar to those of the DEGs in the SP group. In the coexpression network, the commonly downregulated genes (including ND1, ND3, ND4L, and ND6) and the commonly upregulated genes (including TSPY6P and CDY10P) exhibited a higher degree. In addition, 131 DEGs (including ND1, ND3, ND6, MIR449A and TAS2R43) were predicted to be potential pneumonia‑related genes. In conclusion, the present study demonstrated that the common DEGs may be associated with the progression of severe pneumonia.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Marrie TJ: Community-acquired pneumonia. Clin Infect Dis. 18:501–515. 1994. View Article : Google Scholar : PubMed/NCBI

2 

Thomas CP, Ryan M, Chapman JD, Stason WB, Tompkins CP, Suaya JA, Polsky D, Mannino DM and Shepard DS: Incidence and cost of pneumonia in Medicare beneficiaries. Chest. 142:973–981. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Ewig S, Ruiz M, Mensa J, Marcos MA, Martinez JA, Arancibia F, Niederman MS and Torres A: Severe community-acquired pneumonia: Assessment of severity criteria. Am J Respir Crit Care Med. 158:1102–1108. 1998. View Article : Google Scholar : PubMed/NCBI

4 

Nazir SA and Erbland ML: Chronic obstructive pulmonary disease. Drugs Aging. 26:813–831. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Iyer Parameswaran G and Murphy TF: Chronic obstructive pulmonary disease: Role of bacteria and updated guide to antibacterial selection in the older patient. Drugs Aging. 26:985–995. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Calverley PM, Anderson JA, Celli B, Ferguson GT, Jenkins C, Jones PW, Yates JC and Vestbo J: TORCH investigators: Salmeterol and fluticasone propionate and survival in chronic obstructive pulmonary disease. N Engl J Med. 356:775–789. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Singh S, Amin AV and Loke YK: Long-term use of inhaled corticosteroids and the risk of pneumonia in chronic obstructive pulmonary disease: A meta-analysis. Arch Intern Med. 169:219–229. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Eom JS, Song WJ, Yoo H, Jeong BH, Lee HY, Koh WJ, Jeon K and Park HY: Chronic obstructive pulmonary disease severity is associated with severe pneumonia. Ann Thorac Med. 10:105–111. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Liapikou A, Polverino E, Ewig S, Cillóniz C, Marcos MA, Mensa J, Bello S, Martin-Loeches I, Menéndez R and Torres A: Severity and outcomes of hospitalised community-acquired pneumonia in COPD patients. Eur Respir J. 39:855–861. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Zuniga J, Buendía-Roldán I, Zhao Y, Jiménez L, Torres D, Romo J, Ramírez G, Cruz A, Vargas-Alarcon G, Sheu CC, et al: Genetic variants associated with severe pneumonia in A/H1N1 influenza infection. Eur Respir J. 39:604–610. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Kebaier C, Chamberland RR, Allen IC, Gao X, Broglie PM, Hall JD, Jania C, Doerschuk CM, Tilley SL and Duncan JA: Staphylococcus aureus α-hemolysin mediates virulence in a murine model of severe pneumonia through activation of the NLRP3 inflammasome. J Infect Dis. 205:807–817. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Gillet Y, Vanhems P, Lina G, Bes M, Vandenesch F, Floret D and Etienne J: Factors predicting mortality in necrotizing community-acquired pneumonia caused by Staphylococcus aureus containing Panton-Valentine leukocidin. Clin Infect Dis. 45:315–321. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Kilic A, Li H, Stratton CW and Tang YW: Antimicrobial susceptibility patterns and staphylococcal cassette chromosome mec types of, as well as Panton-Valentine leukocidin occurrence among, methicillin-resistant Staphylococcus aureus isolates from children and adults in middle Tennessee. J Clin Microbiol. 44:4436–4440. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Green R, Terclanche A, Becker P, Rheeder P, Wittenberg DF, Anderson R and Masekela R: Cytokine profile and clinical correlates in HIV-exposed infants with severe (hypoxic) pneumonia. South African Respirat J. 22:3–6. 2016. View Article : Google Scholar

15 

Martín-Loeches I, Solé-Violán J, de Castro Rodríguez F, García-Laorden MI, Borderías L, Blanquer J, Rajas O, Briones ML, Aspa J, Herrera-Ramos E, et al: Variants at the promoter of the interleukin-6 gene are associated with severity and outcome of pneumococcal community-acquired pneumonia. Intensive Care Med. 38:256–262. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Association RdbotCm, . The diagnosis and treatment guideline of community-acquired pneumonia. Chin J Tuberc Respir Dis. 29:651–655. 2006.(In Chinese).

17 

Martin M: Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet J. 17:10–12. 2011. View Article : Google Scholar

18 

Robinson MD and Oshlack A: A scaling normalization method for differential expression analysis of RNA-seq data. Genome Biol. 11:R252010. View Article : Google Scholar : PubMed/NCBI

19 

Law CW, Chen Y, Shi W and Smyth GK: Voom: Precision weights unlock linear model analysis tools for RNA-seq read counts. Genome Biol. 15:R292014. View Article : Google Scholar : PubMed/NCBI

20 

Yu G, Wang LG, Han Y and He QY: clusterProfiler: An R package for comparing biological themes among gene clusters. OMICS. 16:284–287. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Routledge R: Fisher's Exact Test. Encyclopedia of Biostatistics. 3:2005. View Article : Google Scholar

22 

Cai H, Chen H, Yi T, Daimon CM, Boyle JP, Peers C, Maudsley S and Martin B: VennPlex-a novel Venn diagram program for comparing and visualizing datasets with differentially regulated datapoints. PLoS One. 8:e533882013. View Article : Google Scholar : PubMed/NCBI

23 

Davis AP, Grondin CJ, Lennon-Hopkins K, Saraceni-Richards C, Sciaky D, King BL, Wiegers TC and Mattingly CJ: The comparative toxicogenomics database's 10th year anniversary: Update 2015. Nucleic Acids Res. 43:D914–D920. 2015. View Article : Google Scholar : PubMed/NCBI

24 

Sparks LM, Xie H, Koza RA, Mynatt R, Hulver MW, Bray GA and Smith SR: A high-fat diet coordinately downregulates genes required for mitochondrial oxidative phosphorylation in skeletal muscle. Diabetes. 54:1926–1933. 2005. View Article : Google Scholar : PubMed/NCBI

25 

Miller JL, Velmurugan K, Cowan MJ and Briken V: The type I NADH dehydrogenase of Mycobacterium tuberculosis counters phagosomal NOX2 activity to inhibit TNF-α-mediated host cell apoptosis. PLoS Pathog. 6:e10008642010. View Article : Google Scholar : PubMed/NCBI

26 

Martín-Gómez L, Villalba A and Abollo E: Identification and expression of immune genes in the flat oyster Ostrea edulis in response to bonamiosis. Gene. 492:81–93. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Khan KN, Stanfield K, Trajkovic D and Harris RK: Cyclooxygenase-2 expression in inflammatory lung lesions of nonhuman primates. Vet Pathol. 37:512–516. 2000. View Article : Google Scholar : PubMed/NCBI

28 

Zaiss AK, Zuber J, Chu C, Machado HB, Jiao J, Catapang AB, Ishikawa TO, Gil JS, Lowe SW and Herschman HR: Reversible suppression of cyclooxygenase 2 (COX-2) expression in vivo by inducible RNA interference. PLoS One. 9:e1012632014. View Article : Google Scholar : PubMed/NCBI

29 

Sadikot RT, Zeng H, Azim AC, Joo M, Dey SK, Breyer RM, Peebles RS, Blackwell TS and Christman JW: Bacterial clearance of Pseudomonas aeruginosa is enhanced by the inhibition of COX-2. Eur J Immunol. 37:1001–1009. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Giacominelli-Stuffler R, Marruchella G, Storelli M, Sabatucci A, Angelucci CB and Maccarrone M: 5-Lipoxygenase and cyclooxygenase-2 in the lungs of pigs naturally affected by enzootic pneumonia and porcine pleuropneumonia. Res Vet Sci. 93:898–903. 2012. View Article : Google Scholar : PubMed/NCBI

31 

Poliseno L, Salmena L, Zhang J, Carver B, Haveman WJ and Pandolfi PP: A coding-independent function of gene and pseudogene mRNAs regulates tumour biology. Nature. 465:1033–1038. 2010. View Article : Google Scholar : PubMed/NCBI

32 

Sarma NJ, Tiriveedhi V, Subramanian V, Shenoy S, Crippin JS, Chapman WC and Mohanakumar T: Hepatitis C virus mediated changes in miRNA-449a modulates inflammatory biomarker YKL40 through components of the NOTCH signaling pathway. PLoS One. 7:e508262012. View Article : Google Scholar : PubMed/NCBI

33 

Wölfle U, Haarhaus B and Schempp CM: Amarogentin displays immunomodulatory effects in human mast cells and keratinocytes. Mediators inflamm. 2015:6301282015. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Feng C, Huang H, Huang S, Zhai YZ, Dong J, Chen L, Huang Z, Zhou X, Li B, Wang LL, Wang LL, et al: Identification of potential key genes associated with severe pneumonia using mRNA‑seq. Exp Ther Med 16: 758-766, 2018.
APA
Feng, C., Huang, H., Huang, S., Zhai, Y., Dong, J., Chen, L. ... Li, T. (2018). Identification of potential key genes associated with severe pneumonia using mRNA‑seq. Experimental and Therapeutic Medicine, 16, 758-766. https://doi.org/10.3892/etm.2018.6262
MLA
Feng, C., Huang, H., Huang, S., Zhai, Y., Dong, J., Chen, L., Huang, Z., Zhou, X., Li, B., Wang, L., Chen, W., Lv, F., Li, T."Identification of potential key genes associated with severe pneumonia using mRNA‑seq". Experimental and Therapeutic Medicine 16.2 (2018): 758-766.
Chicago
Feng, C., Huang, H., Huang, S., Zhai, Y., Dong, J., Chen, L., Huang, Z., Zhou, X., Li, B., Wang, L., Chen, W., Lv, F., Li, T."Identification of potential key genes associated with severe pneumonia using mRNA‑seq". Experimental and Therapeutic Medicine 16, no. 2 (2018): 758-766. https://doi.org/10.3892/etm.2018.6262
Copy and paste a formatted citation
x
Spandidos Publications style
Feng C, Huang H, Huang S, Zhai YZ, Dong J, Chen L, Huang Z, Zhou X, Li B, Wang LL, Wang LL, et al: Identification of potential key genes associated with severe pneumonia using mRNA‑seq. Exp Ther Med 16: 758-766, 2018.
APA
Feng, C., Huang, H., Huang, S., Zhai, Y., Dong, J., Chen, L. ... Li, T. (2018). Identification of potential key genes associated with severe pneumonia using mRNA‑seq. Experimental and Therapeutic Medicine, 16, 758-766. https://doi.org/10.3892/etm.2018.6262
MLA
Feng, C., Huang, H., Huang, S., Zhai, Y., Dong, J., Chen, L., Huang, Z., Zhou, X., Li, B., Wang, L., Chen, W., Lv, F., Li, T."Identification of potential key genes associated with severe pneumonia using mRNA‑seq". Experimental and Therapeutic Medicine 16.2 (2018): 758-766.
Chicago
Feng, C., Huang, H., Huang, S., Zhai, Y., Dong, J., Chen, L., Huang, Z., Zhou, X., Li, B., Wang, L., Chen, W., Lv, F., Li, T."Identification of potential key genes associated with severe pneumonia using mRNA‑seq". Experimental and Therapeutic Medicine 16, no. 2 (2018): 758-766. https://doi.org/10.3892/etm.2018.6262
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team