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Pregabalin treatment for neuropathic pain may damage intervertebral disc tissue

  • Authors:
    • Numan Karaarslan
    • Ibrahim Yilmaz
    • Duygu Yasar Si̇rin
    • Hanefi Ozbek
    • Necati Kaplan
    • Yasin Emre Kaya
    • Yener Akyuva
    • Mehmet Sabri Gurbuz
    • Kadir Oznam
    • Ozkan Ates
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Namik Kemal University School of Medicine, Tekirdag 59100, Turkey, Department of Medical Pharmacology, Istanbul Medipol University School of Medicine, Istanbul 34810, Turkey, Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Namik Kemal University, Tekirdag 59030, Turkey, Department of Neurosurgery, Istanbul Rumeli University, Corlu Reyap Hospital, Tekirdag 59860, Turkey, Department of Orthopedic and Traumatology, Abant Izzet Basal University School of Medicine, Bolu 14000, Turkey, Department of Neurosurgery, Gaziosmanpasa Taksim Training and Research Hospital, Istanbul 34433, Turkey, Department of Neurosurgery, Istanbul Medeniyet University School of Medicine, Istanbul 34730, Turkey, Department of Orthopedic and Traumatology, Istanbul Medipol University School of Medicine, Istanbul 34214, Turkey, Department of Neurosurgery, Istanbul Esenyurt University, Esencan Hospital, Istanbul 34517, Turkey
    Copyright: © Karaarslan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1259-1265
    |
    Published online on: June 12, 2018
       https://doi.org/10.3892/etm.2018.6289
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Abstract

The aim of the present study was to determine whether pharmaceutical preparations with pregabalin (PGB) as an active ingredient, which are widely prescribed by clinicians, exert toxic effects on human primary nucleus pulposus (NP) and annulus fibrosis (AF). Primary human cell cultures were obtained from intact (n=6) and degenerated (n=6) tissues resected from the two groups of patients. Different doses of PGB were applied to these cultures and cells were subjected to molecular analyses at 0, 24 and 48 h. Cell vitality, toxicity and proliferation were assessed using a spectrophotometer. The expression of chondroadherin (CHAD), a (member of the NP‑specific protein family), hypoxia‑inducible factor‑1α (HIF‑1α) and type II collagen (COL2A1) was measured using reverse transcription‑quantitative polymerase chain reaction. The results revealed that cell intensity increased in a time‑dependent manner and cell vitality continued in the cultures without pharmaceuticals. Cell proliferation was suppressed in the PGB‑treated cultures independent from the dose and duration of application. PGB was demonstrated to suppress the expression of CHAD and HIF‑1α. In contrast, COL2A1 gene expression was not revealed in any experimental group. The present study utilized an in vitro model and the PGB active ingredient used herein may not be representative of clinical applications; however, the results demonstrated that PGB has a toxic effect on NP/AF cell cultures containing primary human intervertebral disc tissue. In summary, the use of pharmacological agents containing PGB may suppress the proliferation and differentiation of NP/AF cells and/or tissues, which should be considered when deciding on an appropriate treatment regime.
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Copy and paste a formatted citation
Spandidos Publications style
Karaarslan N, Yilmaz I, Si̇rin DY, Ozbek H, Kaplan N, Kaya YE, Akyuva Y, Gurbuz MS, Oznam K, Ates O, Ates O, et al: Pregabalin treatment for neuropathic pain may damage intervertebral disc tissue. Exp Ther Med 16: 1259-1265, 2018.
APA
Karaarslan, N., Yilmaz, I., Si̇rin, D.Y., Ozbek, H., Kaplan, N., Kaya, Y.E. ... Ates, O. (2018). Pregabalin treatment for neuropathic pain may damage intervertebral disc tissue. Experimental and Therapeutic Medicine, 16, 1259-1265. https://doi.org/10.3892/etm.2018.6289
MLA
Karaarslan, N., Yilmaz, I., Si̇rin, D. Y., Ozbek, H., Kaplan, N., Kaya, Y. E., Akyuva, Y., Gurbuz, M. S., Oznam, K., Ates, O."Pregabalin treatment for neuropathic pain may damage intervertebral disc tissue". Experimental and Therapeutic Medicine 16.2 (2018): 1259-1265.
Chicago
Karaarslan, N., Yilmaz, I., Si̇rin, D. Y., Ozbek, H., Kaplan, N., Kaya, Y. E., Akyuva, Y., Gurbuz, M. S., Oznam, K., Ates, O."Pregabalin treatment for neuropathic pain may damage intervertebral disc tissue". Experimental and Therapeutic Medicine 16, no. 2 (2018): 1259-1265. https://doi.org/10.3892/etm.2018.6289
Copy and paste a formatted citation
x
Spandidos Publications style
Karaarslan N, Yilmaz I, Si̇rin DY, Ozbek H, Kaplan N, Kaya YE, Akyuva Y, Gurbuz MS, Oznam K, Ates O, Ates O, et al: Pregabalin treatment for neuropathic pain may damage intervertebral disc tissue. Exp Ther Med 16: 1259-1265, 2018.
APA
Karaarslan, N., Yilmaz, I., Si̇rin, D.Y., Ozbek, H., Kaplan, N., Kaya, Y.E. ... Ates, O. (2018). Pregabalin treatment for neuropathic pain may damage intervertebral disc tissue. Experimental and Therapeutic Medicine, 16, 1259-1265. https://doi.org/10.3892/etm.2018.6289
MLA
Karaarslan, N., Yilmaz, I., Si̇rin, D. Y., Ozbek, H., Kaplan, N., Kaya, Y. E., Akyuva, Y., Gurbuz, M. S., Oznam, K., Ates, O."Pregabalin treatment for neuropathic pain may damage intervertebral disc tissue". Experimental and Therapeutic Medicine 16.2 (2018): 1259-1265.
Chicago
Karaarslan, N., Yilmaz, I., Si̇rin, D. Y., Ozbek, H., Kaplan, N., Kaya, Y. E., Akyuva, Y., Gurbuz, M. S., Oznam, K., Ates, O."Pregabalin treatment for neuropathic pain may damage intervertebral disc tissue". Experimental and Therapeutic Medicine 16, no. 2 (2018): 1259-1265. https://doi.org/10.3892/etm.2018.6289
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